Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gltX and valU are separated by 258 nucleotides valU and valX are separated by 44 nucleotides valX and valY are separated by 46 nucleotides valY and lysV are separated by 4 nucleotides lysV and xapR are separated by 264 nucleotides xapR and xapB are separated by 251 nucleotides
b2400: gltX - glutamyl-tRNA synthetase (NCBI), at 2,517,279 to 2,518,694
gltX
b2401: valU - tRNA-Val (NCBI), at 2,518,953 to 2,519,028
valU
b2402: valX - tRNA-Val (NCBI), at 2,519,073 to 2,519,148
valX
b2403: valY - tRNA-Val (NCBI), at 2,519,195 to 2,519,270
valY
b2404: lysV - tRNA-Lys (NCBI), at 2,519,275 to 2,519,350
lysV
b2405: xapR - DNA-binding transcriptional activator (NCBI), at 2,519,615 to 2,520,499
xapR
b2406: xapB - xanthosine transporter (NCBI), at 2,520,751 to 2,522,007
xapB
Position (kb)
2519
2520
2521 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2518.799 kb on + strand at 2518.935 kb on - strand at 2518.935 kb on - strand at 2518.939 kb on + strand at 2519.620 kb on - strand at 2519.657 kb on + strand at 2519.665 kb on - strand at 2519.679 kb on + strand at 2519.687 kb on - strand at 2519.788 kb on + strand, within xapR at 2519.788 kb on + strand, within xapR at 2519.891 kb on - strand, within xapR at 2519.891 kb on - strand, within xapR at 2519.891 kb on - strand, within xapR at 2519.970 kb on - strand, within xapR at 2520.091 kb on - strand, within xapR at 2520.091 kb on - strand, within xapR at 2520.181 kb on - strand, within xapR at 2520.188 kb on - strand, within xapR at 2520.195 kb on - strand, within xapR at 2520.207 kb on - strand, within xapR at 2520.208 kb on + strand, within xapR at 2520.208 kb on + strand, within xapR at 2520.224 kb on + strand, within xapR at 2520.224 kb on + strand, within xapR at 2520.245 kb on - strand, within xapR at 2520.288 kb on + strand, within xapR at 2520.303 kb on + strand, within xapR at 2520.311 kb on + strand, within xapR at 2520.311 kb on + strand, within xapR at 2520.313 kb on + strand, within xapR at 2520.313 kb on + strand, within xapR at 2520.313 kb on + strand, within xapR at 2520.313 kb on + strand, within xapR at 2520.324 kb on + strand, within xapR at 2520.336 kb on + strand, within xapR at 2520.336 kb on + strand, within xapR at 2520.337 kb on + strand, within xapR at 2520.344 kb on - strand, within xapR at 2520.454 kb on + strand at 2520.454 kb on + strand at 2520.470 kb on + strand at 2520.480 kb on + strand at 2520.485 kb on + strand at 2520.485 kb on + strand at 2520.485 kb on + strand at 2520.648 kb on - strand at 2520.734 kb on - strand at 2520.734 kb on - strand at 2520.739 kb on - strand at 2520.770 kb on - strand at 2520.863 kb on - strand at 2520.863 kb on - strand at 2520.902 kb on + strand, within xapB at 2520.921 kb on - strand, within xapB at 2520.932 kb on - strand, within xapB at 2520.932 kb on - strand, within xapB at 2520.949 kb on - strand, within xapB at 2520.964 kb on - strand, within xapB at 2520.964 kb on - strand, within xapB at 2521.099 kb on - strand, within xapB at 2521.141 kb on - strand, within xapB at 2521.141 kb on - strand, within xapB at 2521.141 kb on - strand, within xapB at 2521.141 kb on - strand, within xapB at 2521.151 kb on - strand, within xapB at 2521.303 kb on - strand, within xapB at 2521.359 kb on - strand, within xapB at 2521.359 kb on - strand, within xapB at 2521.437 kb on - strand, within xapB
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 2,518,799 + +0.9 2,518,935 - +0.2 2,518,935 - +0.6 2,518,939 + +0.2 2,519,620 - -0.6 2,519,657 + -1.1 2,519,665 - -0.4 2,519,679 + +1.7 2,519,687 - +1.2 2,519,788 + xapR b2405 0.20 +1.2 2,519,788 + xapR b2405 0.20 +0.2 2,519,891 - xapR b2405 0.31 +0.6 2,519,891 - xapR b2405 0.31 -1.9 2,519,891 - xapR b2405 0.31 +0.6 2,519,970 - xapR b2405 0.40 -0.2 2,520,091 - xapR b2405 0.54 -0.5 2,520,091 - xapR b2405 0.54 -2.2 2,520,181 - xapR b2405 0.64 +0.5 2,520,188 - xapR b2405 0.65 -0.6 2,520,195 - xapR b2405 0.66 -1.1 2,520,207 - xapR b2405 0.67 +0.1 2,520,208 + xapR b2405 0.67 +1.9 2,520,208 + xapR b2405 0.67 +1.9 2,520,224 + xapR b2405 0.69 -0.9 2,520,224 + xapR b2405 0.69 +1.8 2,520,245 - xapR b2405 0.71 +0.0 2,520,288 + xapR b2405 0.76 -0.2 2,520,303 + xapR b2405 0.78 +0.0 2,520,311 + xapR b2405 0.79 -1.6 2,520,311 + xapR b2405 0.79 -0.8 2,520,313 + xapR b2405 0.79 +0.2 2,520,313 + xapR b2405 0.79 -2.4 2,520,313 + xapR b2405 0.79 -0.2 2,520,313 + xapR b2405 0.79 +0.5 2,520,324 + xapR b2405 0.80 +2.7 2,520,336 + xapR b2405 0.81 +2.2 2,520,336 + xapR b2405 0.81 -0.4 2,520,337 + xapR b2405 0.82 -2.1 2,520,344 - xapR b2405 0.82 -1.1 2,520,454 + +1.0 2,520,454 + -1.5 2,520,470 + +0.0 2,520,480 + -0.8 2,520,485 + -0.4 2,520,485 + -1.1 2,520,485 + +0.6 2,520,648 - +0.9 2,520,734 - -0.6 2,520,734 - -1.2 2,520,739 - -0.4 2,520,770 - -1.7 2,520,863 - -0.8 2,520,863 - -0.8 2,520,902 + xapB b2406 0.12 -0.6 2,520,921 - xapB b2406 0.14 -2.5 2,520,932 - xapB b2406 0.14 -0.8 2,520,932 - xapB b2406 0.14 +1.6 2,520,949 - xapB b2406 0.16 +0.8 2,520,964 - xapB b2406 0.17 +0.5 2,520,964 - xapB b2406 0.17 -0.8 2,521,099 - xapB b2406 0.28 -2.1 2,521,141 - xapB b2406 0.31 -1.3 2,521,141 - xapB b2406 0.31 -0.8 2,521,141 - xapB b2406 0.31 -1.4 2,521,141 - xapB b2406 0.31 -1.6 2,521,151 - xapB b2406 0.32 -0.4 2,521,303 - xapB b2406 0.44 -0.6 2,521,359 - xapB b2406 0.48 +4.3 2,521,359 - xapB b2406 0.48 +1.8 2,521,437 - xapB b2406 0.55 +2.1
Or see this region's nucleotide sequence