Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfdL and yfdM overlap by 5 nucleotides yfdM and yfdN overlap by 1 nucleotides yfdN and oweS overlap by 4 nucleotides oweS and yfdP are separated by 18 nucleotides yfdP and yfdQ are separated by 65 nucleotides
b2355: yfdL - CPS-53 (KpLE1) prophage; conserved protein (RefSeq), at 2,469,566 to 2,470,144
yfdL
b2356: yfdM - orf, hypothetical protein (VIMSS), at 2,470,140 to 2,470,409
yfdM
b2357: yfdN - CPS-53 (KpLE1) prophage; predicted protein (NCBI), at 2,470,409 to 2,470,903
yfdN
b2358: oweS - CPS-53 (KpLE1) prophage; predicted defective phage replication protein O (RefSeq), at 2,470,900 to 2,471,607
oweS
b2359: yfdP - CPS-53 (KpLE1) prophage; predicted protein (RefSeq), at 2,471,626 to 2,471,988
yfdP
b2360: yfdQ - CPS-53 (KpLE1) prophage; predicted protein (NCBI), at 2,472,054 to 2,472,878
yfdQ
Position (kb)
2470
2471
2472 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2469.940 kb on - strand, within yfdL at 2469.942 kb on - strand, within yfdL at 2469.942 kb on - strand, within yfdL at 2469.998 kb on + strand, within yfdL at 2470.038 kb on - strand, within yfdL at 2470.038 kb on - strand, within yfdL at 2470.049 kb on - strand, within yfdL at 2470.049 kb on - strand, within yfdL at 2470.090 kb on - strand at 2470.168 kb on + strand, within yfdM at 2470.211 kb on - strand, within yfdM at 2470.448 kb on + strand at 2470.448 kb on + strand at 2470.477 kb on + strand, within yfdN at 2470.477 kb on + strand, within yfdN at 2470.484 kb on - strand, within yfdN at 2470.484 kb on - strand, within yfdN at 2470.741 kb on - strand, within yfdN at 2470.913 kb on - strand at 2470.928 kb on + strand at 2471.021 kb on - strand, within oweS at 2471.021 kb on - strand, within oweS at 2471.090 kb on + strand, within oweS at 2471.133 kb on + strand, within oweS at 2471.133 kb on + strand, within oweS at 2471.201 kb on + strand, within oweS at 2471.201 kb on + strand, within oweS at 2471.263 kb on + strand, within oweS at 2471.277 kb on - strand, within oweS at 2471.277 kb on - strand, within oweS at 2471.372 kb on + strand, within oweS at 2471.372 kb on + strand, within oweS at 2471.526 kb on + strand, within oweS at 2471.539 kb on - strand at 2471.605 kb on + strand at 2471.605 kb on + strand at 2471.765 kb on - strand, within yfdP at 2471.783 kb on + strand, within yfdP at 2471.835 kb on + strand, within yfdP at 2471.841 kb on - strand, within yfdP at 2471.906 kb on + strand, within yfdP at 2471.906 kb on + strand, within yfdP at 2471.909 kb on + strand, within yfdP at 2471.909 kb on + strand, within yfdP at 2471.913 kb on + strand, within yfdP at 2471.936 kb on + strand, within yfdP at 2471.936 kb on + strand, within yfdP at 2471.945 kb on + strand, within yfdP at 2471.945 kb on + strand, within yfdP at 2471.970 kb on + strand at 2471.970 kb on + strand at 2472.031 kb on + strand at 2472.074 kb on + strand at 2472.223 kb on - strand, within yfdQ at 2472.227 kb on + strand, within yfdQ at 2472.228 kb on - strand, within yfdQ at 2472.235 kb on - strand, within yfdQ at 2472.235 kb on - strand, within yfdQ at 2472.258 kb on - strand, within yfdQ at 2472.272 kb on - strand, within yfdQ at 2472.287 kb on - strand, within yfdQ at 2472.287 kb on - strand, within yfdQ at 2472.297 kb on - strand, within yfdQ at 2472.303 kb on - strand, within yfdQ at 2472.309 kb on + strand, within yfdQ at 2472.309 kb on + strand, within yfdQ at 2472.317 kb on - strand, within yfdQ at 2472.317 kb on - strand, within yfdQ at 2472.336 kb on + strand, within yfdQ at 2472.337 kb on + strand, within yfdQ at 2472.337 kb on + strand, within yfdQ at 2472.339 kb on - strand, within yfdQ at 2472.340 kb on - strand, within yfdQ at 2472.343 kb on - strand, within yfdQ at 2472.346 kb on + strand, within yfdQ at 2472.346 kb on - strand, within yfdQ at 2472.353 kb on - strand, within yfdQ at 2472.362 kb on - strand, within yfdQ at 2472.363 kb on - strand, within yfdQ at 2472.367 kb on - strand, within yfdQ at 2472.367 kb on - strand, within yfdQ at 2472.404 kb on + strand, within yfdQ at 2472.410 kb on - strand, within yfdQ at 2472.410 kb on - strand, within yfdQ at 2472.417 kb on - strand, within yfdQ at 2472.417 kb on - strand, within yfdQ at 2472.451 kb on - strand, within yfdQ at 2472.490 kb on - strand, within yfdQ at 2472.492 kb on - strand, within yfdQ at 2472.511 kb on - strand, within yfdQ at 2472.577 kb on - strand, within yfdQ at 2472.577 kb on - strand, within yfdQ at 2472.578 kb on - strand, within yfdQ at 2472.591 kb on - strand, within yfdQ
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 2,469,940 - yfdL b2355 0.65 -0.6 2,469,942 - yfdL b2355 0.65 +1.6 2,469,942 - yfdL b2355 0.65 -0.7 2,469,998 + yfdL b2355 0.75 +2.2 2,470,038 - yfdL b2355 0.82 -1.0 2,470,038 - yfdL b2355 0.82 +0.2 2,470,049 - yfdL b2355 0.83 +0.4 2,470,049 - yfdL b2355 0.83 -0.1 2,470,090 - +0.5 2,470,168 + yfdM b2356 0.10 +3.0 2,470,211 - yfdM b2356 0.26 +1.9 2,470,448 + +3.0 2,470,448 + +1.2 2,470,477 + yfdN b2357 0.14 +0.3 2,470,477 + yfdN b2357 0.14 +0.9 2,470,484 - yfdN b2357 0.15 -2.6 2,470,484 - yfdN b2357 0.15 +1.1 2,470,741 - yfdN b2357 0.67 +1.2 2,470,913 - -0.8 2,470,928 + +0.6 2,471,021 - oweS b2358 0.17 -1.1 2,471,021 - oweS b2358 0.17 -0.2 2,471,090 + oweS b2358 0.27 +0.4 2,471,133 + oweS b2358 0.33 +0.2 2,471,133 + oweS b2358 0.33 +1.2 2,471,201 + oweS b2358 0.43 -1.2 2,471,201 + oweS b2358 0.43 -0.1 2,471,263 + oweS b2358 0.51 -1.1 2,471,277 - oweS b2358 0.53 -0.8 2,471,277 - oweS b2358 0.53 +1.8 2,471,372 + oweS b2358 0.67 -0.6 2,471,372 + oweS b2358 0.67 +0.2 2,471,526 + oweS b2358 0.88 -1.9 2,471,539 - +0.5 2,471,605 + -1.8 2,471,605 + -0.4 2,471,765 - yfdP b2359 0.38 +0.7 2,471,783 + yfdP b2359 0.43 +2.3 2,471,835 + yfdP b2359 0.58 +1.6 2,471,841 - yfdP b2359 0.59 -0.0 2,471,906 + yfdP b2359 0.77 -0.1 2,471,906 + yfdP b2359 0.77 +1.1 2,471,909 + yfdP b2359 0.78 +3.8 2,471,909 + yfdP b2359 0.78 -0.8 2,471,913 + yfdP b2359 0.79 -2.9 2,471,936 + yfdP b2359 0.85 +1.7 2,471,936 + yfdP b2359 0.85 -1.2 2,471,945 + yfdP b2359 0.88 +1.2 2,471,945 + yfdP b2359 0.88 -0.4 2,471,970 + +1.2 2,471,970 + -1.6 2,472,031 + -1.8 2,472,074 + +0.7 2,472,223 - yfdQ b2360 0.20 +0.1 2,472,227 + yfdQ b2360 0.21 -1.8 2,472,228 - yfdQ b2360 0.21 -1.8 2,472,235 - yfdQ b2360 0.22 -1.8 2,472,235 - yfdQ b2360 0.22 -0.6 2,472,258 - yfdQ b2360 0.25 +1.6 2,472,272 - yfdQ b2360 0.26 +2.1 2,472,287 - yfdQ b2360 0.28 +0.2 2,472,287 - yfdQ b2360 0.28 +1.4 2,472,297 - yfdQ b2360 0.29 +0.7 2,472,303 - yfdQ b2360 0.30 -0.8 2,472,309 + yfdQ b2360 0.31 +1.6 2,472,309 + yfdQ b2360 0.31 -0.1 2,472,317 - yfdQ b2360 0.32 +0.6 2,472,317 - yfdQ b2360 0.32 -1.8 2,472,336 + yfdQ b2360 0.34 -1.6 2,472,337 + yfdQ b2360 0.34 -0.2 2,472,337 + yfdQ b2360 0.34 -2.1 2,472,339 - yfdQ b2360 0.35 -1.7 2,472,340 - yfdQ b2360 0.35 -0.5 2,472,343 - yfdQ b2360 0.35 -0.6 2,472,346 + yfdQ b2360 0.35 -1.4 2,472,346 - yfdQ b2360 0.35 +3.0 2,472,353 - yfdQ b2360 0.36 +2.3 2,472,362 - yfdQ b2360 0.37 -0.2 2,472,363 - yfdQ b2360 0.37 +0.5 2,472,367 - yfdQ b2360 0.38 -1.4 2,472,367 - yfdQ b2360 0.38 +1.2 2,472,404 + yfdQ b2360 0.42 -0.7 2,472,410 - yfdQ b2360 0.43 +2.2 2,472,410 - yfdQ b2360 0.43 +1.2 2,472,417 - yfdQ b2360 0.44 +0.2 2,472,417 - yfdQ b2360 0.44 -1.8 2,472,451 - yfdQ b2360 0.48 +1.5 2,472,490 - yfdQ b2360 0.53 +0.2 2,472,492 - yfdQ b2360 0.53 -0.4 2,472,511 - yfdQ b2360 0.55 +0.2 2,472,577 - yfdQ b2360 0.63 -2.1 2,472,577 - yfdQ b2360 0.63 +0.4 2,472,578 - yfdQ b2360 0.64 +0.0 2,472,591 - yfdQ b2360 0.65 -0.5
Or see this region's nucleotide sequence