Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfcI and hisP are separated by 196 nucleotides hisP and hisM are separated by 7 nucleotides hisM and hisQ overlap by 4 nucleotides
b2305: yfcI - hypothetical protein (NCBI), at 2,420,671 to 2,421,561
yfcI
b2306: hisP - histidine/lysine/arginine/ornithine transporter subunit (NCBI), at 2,421,758 to 2,422,531
hisP
b2307: hisM - histidine/lysine/arginine/ornithine transporter subunit (NCBI), at 2,422,539 to 2,423,255
hisM
b2308: hisQ - histidine/lysine/arginine/ornithine transporter subunit (NCBI), at 2,423,252 to 2,423,938
hisQ
Position (kb)
2421
2422
2423 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2420.758 kb on - strand at 2420.758 kb on - strand at 2420.772 kb on + strand, within yfcI at 2420.775 kb on - strand, within yfcI at 2420.775 kb on - strand, within yfcI at 2420.784 kb on - strand, within yfcI at 2420.801 kb on - strand, within yfcI at 2420.932 kb on + strand, within yfcI at 2420.932 kb on + strand, within yfcI at 2420.953 kb on - strand, within yfcI at 2420.966 kb on - strand, within yfcI at 2420.987 kb on + strand, within yfcI at 2420.990 kb on + strand, within yfcI at 2420.990 kb on + strand at 2420.990 kb on + strand, within yfcI at 2420.998 kb on - strand, within yfcI at 2421.000 kb on - strand, within yfcI at 2421.008 kb on - strand, within yfcI at 2421.052 kb on + strand, within yfcI at 2421.052 kb on - strand, within yfcI at 2421.054 kb on + strand, within yfcI at 2421.054 kb on + strand, within yfcI at 2421.055 kb on - strand, within yfcI at 2421.057 kb on + strand, within yfcI at 2421.060 kb on - strand, within yfcI at 2421.060 kb on - strand, within yfcI at 2421.063 kb on - strand, within yfcI at 2421.063 kb on - strand, within yfcI at 2421.075 kb on + strand, within yfcI at 2421.075 kb on + strand, within yfcI at 2421.089 kb on - strand, within yfcI at 2421.089 kb on - strand, within yfcI at 2421.129 kb on + strand, within yfcI at 2421.134 kb on - strand, within yfcI at 2421.146 kb on - strand, within yfcI at 2421.178 kb on - strand, within yfcI at 2421.179 kb on + strand, within yfcI at 2421.179 kb on + strand, within yfcI at 2421.210 kb on + strand, within yfcI at 2421.225 kb on + strand, within yfcI at 2421.225 kb on + strand, within yfcI at 2421.226 kb on + strand, within yfcI at 2421.227 kb on + strand, within yfcI at 2421.227 kb on + strand, within yfcI at 2421.237 kb on + strand, within yfcI at 2421.335 kb on - strand, within yfcI at 2421.345 kb on - strand, within yfcI at 2421.370 kb on - strand, within yfcI at 2421.370 kb on - strand, within yfcI at 2421.385 kb on - strand, within yfcI at 2421.425 kb on - strand, within yfcI at 2421.425 kb on - strand, within yfcI at 2421.425 kb on - strand, within yfcI at 2421.431 kb on - strand, within yfcI at 2421.437 kb on + strand, within yfcI at 2421.439 kb on + strand, within yfcI at 2421.445 kb on + strand, within yfcI at 2421.457 kb on - strand, within yfcI at 2421.457 kb on - strand, within yfcI at 2421.494 kb on + strand at 2421.494 kb on + strand at 2421.502 kb on - strand at 2421.503 kb on - strand at 2421.504 kb on - strand at 2421.535 kb on - strand at 2421.535 kb on - strand at 2421.694 kb on + strand at 2421.771 kb on + strand at 2421.782 kb on - strand at 2421.790 kb on - strand at 2421.793 kb on - strand at 2421.793 kb on - strand at 2421.793 kb on - strand at 2421.793 kb on - strand at 2421.806 kb on - strand at 2421.860 kb on - strand, within hisP at 2421.874 kb on - strand, within hisP at 2421.918 kb on - strand, within hisP at 2421.921 kb on + strand, within hisP at 2421.928 kb on - strand, within hisP at 2421.928 kb on - strand, within hisP at 2421.963 kb on + strand, within hisP at 2421.963 kb on + strand, within hisP at 2421.971 kb on - strand, within hisP at 2421.973 kb on + strand, within hisP at 2421.981 kb on - strand, within hisP at 2421.981 kb on - strand, within hisP at 2422.005 kb on - strand, within hisP at 2422.199 kb on + strand, within hisP at 2422.199 kb on + strand, within hisP at 2422.218 kb on - strand, within hisP at 2422.232 kb on + strand, within hisP at 2422.232 kb on + strand, within hisP at 2422.255 kb on - strand, within hisP at 2422.255 kb on - strand, within hisP at 2422.375 kb on + strand, within hisP at 2422.486 kb on - strand at 2422.538 kb on - strand at 2422.538 kb on - strand at 2422.607 kb on + strand at 2422.607 kb on + strand at 2422.607 kb on + strand at 2422.621 kb on + strand, within hisM at 2422.627 kb on - strand, within hisM at 2422.684 kb on - strand, within hisM at 2422.684 kb on - strand, within hisM at 2422.684 kb on - strand, within hisM at 2422.711 kb on - strand, within hisM at 2422.712 kb on - strand, within hisM at 2422.730 kb on + strand, within hisM at 2422.738 kb on - strand, within hisM at 2422.740 kb on + strand, within hisM at 2422.740 kb on + strand, within hisM at 2422.765 kb on + strand, within hisM at 2422.765 kb on - strand, within hisM at 2422.788 kb on - strand, within hisM at 2422.798 kb on - strand, within hisM at 2422.836 kb on + strand, within hisM at 2422.850 kb on - strand, within hisM at 2422.850 kb on - strand, within hisM at 2422.904 kb on - strand, within hisM at 2422.942 kb on + strand, within hisM at 2422.947 kb on - strand, within hisM at 2422.962 kb on - strand, within hisM at 2423.022 kb on + strand, within hisM at 2423.038 kb on + strand, within hisM at 2423.041 kb on - strand, within hisM at 2423.145 kb on + strand, within hisM at 2423.145 kb on + strand, within hisM at 2423.199 kb on - strand at 2423.199 kb on - strand at 2423.233 kb on - strand at 2423.240 kb on - strand at 2423.240 kb on - strand at 2423.265 kb on - strand at 2423.265 kb on - strand at 2423.267 kb on + strand at 2423.280 kb on + strand at 2423.280 kb on + strand at 2423.310 kb on - strand at 2423.310 kb on - strand at 2423.346 kb on + strand, within hisQ at 2423.421 kb on - strand, within hisQ at 2423.426 kb on + strand, within hisQ at 2423.490 kb on + strand, within hisQ
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 2,420,758 - -2.2 2,420,758 - -0.4 2,420,772 + yfcI b2305 0.11 +2.6 2,420,775 - yfcI b2305 0.12 -0.5 2,420,775 - yfcI b2305 0.12 -0.4 2,420,784 - yfcI b2305 0.13 +1.5 2,420,801 - yfcI b2305 0.15 -0.6 2,420,932 + yfcI b2305 0.29 -0.6 2,420,932 + yfcI b2305 0.29 -1.8 2,420,953 - yfcI b2305 0.32 +0.1 2,420,966 - yfcI b2305 0.33 -0.1 2,420,987 + yfcI b2305 0.35 -0.9 2,420,990 + yfcI b2305 0.36 -1.4 2,420,990 + +0.2 2,420,990 + yfcI b2305 0.36 +4.2 2,420,998 - yfcI b2305 0.37 -2.3 2,421,000 - yfcI b2305 0.37 -0.3 2,421,008 - yfcI b2305 0.38 -0.4 2,421,052 + yfcI b2305 0.43 +1.1 2,421,052 - yfcI b2305 0.43 +0.3 2,421,054 + yfcI b2305 0.43 -0.5 2,421,054 + yfcI b2305 0.43 +0.6 2,421,055 - yfcI b2305 0.43 -2.2 2,421,057 + yfcI b2305 0.43 +1.8 2,421,060 - yfcI b2305 0.44 -1.8 2,421,060 - yfcI b2305 0.44 -1.1 2,421,063 - yfcI b2305 0.44 +1.5 2,421,063 - yfcI b2305 0.44 +1.9 2,421,075 + yfcI b2305 0.45 -0.6 2,421,075 + yfcI b2305 0.45 -1.3 2,421,089 - yfcI b2305 0.47 -0.9 2,421,089 - yfcI b2305 0.47 +2.6 2,421,129 + yfcI b2305 0.51 +0.0 2,421,134 - yfcI b2305 0.52 +2.0 2,421,146 - yfcI b2305 0.53 +2.5 2,421,178 - yfcI b2305 0.57 -1.9 2,421,179 + yfcI b2305 0.57 +0.5 2,421,179 + yfcI b2305 0.57 -0.3 2,421,210 + yfcI b2305 0.60 -1.4 2,421,225 + yfcI b2305 0.62 +1.5 2,421,225 + yfcI b2305 0.62 -0.9 2,421,226 + yfcI b2305 0.62 +0.7 2,421,227 + yfcI b2305 0.62 +0.1 2,421,227 + yfcI b2305 0.62 +0.2 2,421,237 + yfcI b2305 0.64 +2.0 2,421,335 - yfcI b2305 0.75 -1.6 2,421,345 - yfcI b2305 0.76 +1.4 2,421,370 - yfcI b2305 0.78 +0.6 2,421,370 - yfcI b2305 0.78 -2.0 2,421,385 - yfcI b2305 0.80 -0.8 2,421,425 - yfcI b2305 0.85 -0.6 2,421,425 - yfcI b2305 0.85 +0.3 2,421,425 - yfcI b2305 0.85 +3.3 2,421,431 - yfcI b2305 0.85 +1.0 2,421,437 + yfcI b2305 0.86 -2.5 2,421,439 + yfcI b2305 0.86 +0.4 2,421,445 + yfcI b2305 0.87 -0.5 2,421,457 - yfcI b2305 0.88 +3.5 2,421,457 - yfcI b2305 0.88 +0.0 2,421,494 + -0.8 2,421,494 + -0.9 2,421,502 - -3.5 2,421,503 - +0.6 2,421,504 - -1.1 2,421,535 - +0.8 2,421,535 - +0.1 2,421,694 + +1.0 2,421,771 + -0.4 2,421,782 - -1.7 2,421,790 - -3.2 2,421,793 - -0.4 2,421,793 - +0.2 2,421,793 - -0.5 2,421,793 - +0.1 2,421,806 - -0.0 2,421,860 - hisP b2306 0.13 -0.9 2,421,874 - hisP b2306 0.15 -1.5 2,421,918 - hisP b2306 0.21 +0.5 2,421,921 + hisP b2306 0.21 -2.1 2,421,928 - hisP b2306 0.22 +0.1 2,421,928 - hisP b2306 0.22 +0.4 2,421,963 + hisP b2306 0.26 -1.2 2,421,963 + hisP b2306 0.26 -0.2 2,421,971 - hisP b2306 0.28 +1.5 2,421,973 + hisP b2306 0.28 -0.8 2,421,981 - hisP b2306 0.29 -0.4 2,421,981 - hisP b2306 0.29 +0.6 2,422,005 - hisP b2306 0.32 -0.6 2,422,199 + hisP b2306 0.57 -0.5 2,422,199 + hisP b2306 0.57 +0.5 2,422,218 - hisP b2306 0.59 -3.0 2,422,232 + hisP b2306 0.61 -1.1 2,422,232 + hisP b2306 0.61 -1.1 2,422,255 - hisP b2306 0.64 +0.2 2,422,255 - hisP b2306 0.64 +0.2 2,422,375 + hisP b2306 0.80 -3.0 2,422,486 - +1.0 2,422,538 - +1.2 2,422,538 - -0.0 2,422,607 + -0.1 2,422,607 + +0.8 2,422,607 + -0.4 2,422,621 + hisM b2307 0.11 +0.0 2,422,627 - hisM b2307 0.12 +1.9 2,422,684 - hisM b2307 0.20 +0.2 2,422,684 - hisM b2307 0.20 +0.3 2,422,684 - hisM b2307 0.20 +1.8 2,422,711 - hisM b2307 0.24 -0.4 2,422,712 - hisM b2307 0.24 -0.8 2,422,730 + hisM b2307 0.27 +0.2 2,422,738 - hisM b2307 0.28 -1.1 2,422,740 + hisM b2307 0.28 +0.2 2,422,740 + hisM b2307 0.28 -0.1 2,422,765 + hisM b2307 0.32 -0.7 2,422,765 - hisM b2307 0.32 -1.1 2,422,788 - hisM b2307 0.35 -1.6 2,422,798 - hisM b2307 0.36 +0.5 2,422,836 + hisM b2307 0.41 -1.0 2,422,850 - hisM b2307 0.43 +0.8 2,422,850 - hisM b2307 0.43 -1.4 2,422,904 - hisM b2307 0.51 -1.0 2,422,942 + hisM b2307 0.56 -0.3 2,422,947 - hisM b2307 0.57 +0.3 2,422,962 - hisM b2307 0.59 -0.3 2,423,022 + hisM b2307 0.67 -0.8 2,423,038 + hisM b2307 0.70 +1.3 2,423,041 - hisM b2307 0.70 -2.2 2,423,145 + hisM b2307 0.85 -0.4 2,423,145 + hisM b2307 0.85 +1.9 2,423,199 - +1.8 2,423,199 - +0.8 2,423,233 - -0.1 2,423,240 - -0.6 2,423,240 - +1.2 2,423,265 - +1.8 2,423,265 - +0.0 2,423,267 + +0.8 2,423,280 + -2.1 2,423,280 + -0.8 2,423,310 - -0.4 2,423,310 - +1.8 2,423,346 + hisQ b2308 0.14 +0.0 2,423,421 - hisQ b2308 0.25 +1.7 2,423,426 + hisQ b2308 0.25 +1.0 2,423,490 + hisQ b2308 0.35 -0.4
Or see this region's nucleotide sequence