Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfcD and yfcE are separated by 57 nucleotides yfcE and yfcF are separated by 52 nucleotides yfcF and yfcG are separated by 135 nucleotides yfcG and folX are separated by 56 nucleotides
b2299: yfcD - predicted NUDIX hydrolase (NCBI), at 2,416,656 to 2,417,198
yfcD
b2300: yfcE - orf, hypothetical protein (VIMSS), at 2,417,256 to 2,417,810
yfcE
b2301: yfcF - predicted enzyme (NCBI), at 2,417,863 to 2,418,507
yfcF
b2302: yfcG - predicted glutathione S-transferase (NCBI), at 2,418,643 to 2,419,290
yfcG
b2303: folX - D-erythro-7,8-dihydroneopterin triphosphate 2'-epimerase and dihydroneopterin aldolase (NCBI), at 2,419,347 to 2,419,709
folX
Position (kb)
2417
2418
2419 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2416.973 kb on + strand, within yfcD at 2416.977 kb on - strand, within yfcD at 2416.977 kb on - strand, within yfcD at 2416.977 kb on - strand, within yfcD at 2417.064 kb on + strand, within yfcD at 2417.124 kb on + strand, within yfcD at 2417.124 kb on + strand, within yfcD at 2417.162 kb on - strand at 2417.177 kb on + strand at 2417.225 kb on + strand at 2417.235 kb on - strand at 2417.408 kb on + strand, within yfcE at 2417.730 kb on + strand, within yfcE at 2417.745 kb on + strand, within yfcE at 2417.745 kb on + strand, within yfcE at 2417.753 kb on - strand, within yfcE at 2417.753 kb on - strand, within yfcE at 2417.759 kb on - strand at 2417.965 kb on + strand, within yfcF at 2417.965 kb on + strand, within yfcF at 2417.970 kb on - strand, within yfcF at 2417.970 kb on - strand, within yfcF at 2418.048 kb on - strand, within yfcF at 2418.133 kb on + strand, within yfcF at 2418.153 kb on + strand, within yfcF at 2418.184 kb on - strand, within yfcF at 2418.247 kb on + strand, within yfcF at 2418.247 kb on + strand, within yfcF at 2418.269 kb on + strand, within yfcF at 2418.269 kb on + strand, within yfcF at 2418.445 kb on - strand at 2418.572 kb on - strand at 2418.607 kb on - strand at 2418.607 kb on - strand at 2418.607 kb on - strand at 2418.607 kb on - strand at 2418.714 kb on + strand, within yfcG at 2418.736 kb on + strand, within yfcG at 2418.739 kb on - strand, within yfcG at 2418.739 kb on - strand, within yfcG at 2418.744 kb on - strand, within yfcG at 2418.876 kb on - strand, within yfcG at 2418.934 kb on - strand, within yfcG at 2418.939 kb on - strand, within yfcG at 2418.970 kb on - strand, within yfcG at 2419.017 kb on - strand, within yfcG at 2419.052 kb on - strand, within yfcG at 2419.064 kb on + strand, within yfcG at 2419.077 kb on - strand, within yfcG at 2419.077 kb on - strand, within yfcG at 2419.108 kb on - strand, within yfcG at 2419.109 kb on - strand, within yfcG at 2419.186 kb on + strand, within yfcG at 2419.188 kb on + strand, within yfcG at 2419.213 kb on + strand, within yfcG at 2419.213 kb on + strand, within yfcG at 2419.222 kb on + strand, within yfcG at 2419.223 kb on - strand, within yfcG at 2419.241 kb on + strand at 2419.251 kb on + strand at 2419.267 kb on + strand at 2419.349 kb on + strand at 2419.398 kb on - strand, within folX at 2419.398 kb on - strand, within folX at 2419.452 kb on + strand, within folX at 2419.452 kb on + strand, within folX at 2419.458 kb on + strand, within folX at 2419.458 kb on + strand, within folX at 2419.458 kb on + strand, within folX at 2419.458 kb on + strand, within folX at 2419.458 kb on - strand, within folX at 2419.458 kb on - strand, within folX at 2419.460 kb on - strand, within folX at 2419.460 kb on - strand, within folX at 2419.466 kb on - strand, within folX at 2419.466 kb on - strand, within folX at 2419.468 kb on - strand, within folX at 2419.468 kb on - strand, within folX at 2419.471 kb on + strand, within folX at 2419.471 kb on + strand, within folX at 2419.478 kb on + strand, within folX at 2419.494 kb on + strand, within folX
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 2,416,973 + yfcD b2299 0.58 -0.8 2,416,977 - yfcD b2299 0.59 +1.6 2,416,977 - yfcD b2299 0.59 -0.4 2,416,977 - yfcD b2299 0.59 -0.1 2,417,064 + yfcD b2299 0.75 +3.4 2,417,124 + yfcD b2299 0.86 +1.7 2,417,124 + yfcD b2299 0.86 +1.9 2,417,162 - -0.8 2,417,177 + +0.6 2,417,225 + -1.4 2,417,235 - -2.2 2,417,408 + yfcE b2300 0.27 -0.3 2,417,730 + yfcE b2300 0.85 +0.9 2,417,745 + yfcE b2300 0.88 -0.1 2,417,745 + yfcE b2300 0.88 +1.8 2,417,753 - yfcE b2300 0.90 -1.1 2,417,753 - yfcE b2300 0.90 -0.4 2,417,759 - -0.8 2,417,965 + yfcF b2301 0.16 +0.6 2,417,965 + yfcF b2301 0.16 -1.9 2,417,970 - yfcF b2301 0.17 -2.2 2,417,970 - yfcF b2301 0.17 -1.6 2,418,048 - yfcF b2301 0.29 -0.8 2,418,133 + yfcF b2301 0.42 +2.0 2,418,153 + yfcF b2301 0.45 +0.2 2,418,184 - yfcF b2301 0.50 -1.1 2,418,247 + yfcF b2301 0.60 +0.5 2,418,247 + yfcF b2301 0.60 +1.2 2,418,269 + yfcF b2301 0.63 -0.6 2,418,269 + yfcF b2301 0.63 -0.8 2,418,445 - +0.7 2,418,572 - -0.4 2,418,607 - -0.9 2,418,607 - -1.2 2,418,607 - -2.2 2,418,607 - -0.6 2,418,714 + yfcG b2302 0.11 -0.7 2,418,736 + yfcG b2302 0.14 +0.5 2,418,739 - yfcG b2302 0.15 +0.4 2,418,739 - yfcG b2302 0.15 +1.8 2,418,744 - yfcG b2302 0.16 -0.8 2,418,876 - yfcG b2302 0.36 -2.6 2,418,934 - yfcG b2302 0.45 -0.6 2,418,939 - yfcG b2302 0.46 -0.6 2,418,970 - yfcG b2302 0.50 +1.1 2,419,017 - yfcG b2302 0.58 -0.6 2,419,052 - yfcG b2302 0.63 +0.2 2,419,064 + yfcG b2302 0.65 +2.3 2,419,077 - yfcG b2302 0.67 -0.2 2,419,077 - yfcG b2302 0.67 -0.5 2,419,108 - yfcG b2302 0.72 -0.8 2,419,109 - yfcG b2302 0.72 -0.8 2,419,186 + yfcG b2302 0.84 -1.4 2,419,188 + yfcG b2302 0.84 +0.7 2,419,213 + yfcG b2302 0.88 -1.6 2,419,213 + yfcG b2302 0.88 -0.8 2,419,222 + yfcG b2302 0.89 +0.2 2,419,223 - yfcG b2302 0.90 -0.7 2,419,241 + +0.0 2,419,251 + -0.2 2,419,267 + -0.8 2,419,349 + +0.4 2,419,398 - folX b2303 0.14 -1.8 2,419,398 - folX b2303 0.14 -1.2 2,419,452 + folX b2303 0.29 -1.1 2,419,452 + folX b2303 0.29 -2.1 2,419,458 + folX b2303 0.31 -3.6 2,419,458 + folX b2303 0.31 -0.3 2,419,458 + folX b2303 0.31 +1.1 2,419,458 + folX b2303 0.31 +2.1 2,419,458 - folX b2303 0.31 -1.8 2,419,458 - folX b2303 0.31 +1.7 2,419,460 - folX b2303 0.31 -0.4 2,419,460 - folX b2303 0.31 -0.1 2,419,466 - folX b2303 0.33 +0.2 2,419,466 - folX b2303 0.33 +2.0 2,419,468 - folX b2303 0.33 -0.8 2,419,468 - folX b2303 0.33 -1.4 2,419,471 + folX b2303 0.34 -1.2 2,419,471 + folX b2303 0.34 -1.2 2,419,478 + folX b2303 0.36 -1.1 2,419,494 + folX b2303 0.40 +4.6
Or see this region's nucleotide sequence