Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ccmE and ccmD overlap by 4 nucleotides ccmD and ccmC overlap by 4 nucleotides ccmC and ccmB are separated by 41 nucleotides ccmB and ccmA overlap by 4 nucleotides ccmA and napC are separated by 12 nucleotides
b2197: ccmE - periplasmic heme chaperone (NCBI), at 2,292,923 to 2,293,402
ccmE
b2198: ccmD - cytochrome c biogenesis protein (NCBI), at 2,293,399 to 2,293,608
ccmD
b2199: ccmC - heme exporter subunit (NCBI), at 2,293,605 to 2,294,342
ccmC
b2200: ccmB - heme exporter subunit (NCBI), at 2,294,384 to 2,295,046
ccmB
b2201: ccmA - ATP binding protein of heme exporter A (VIMSS), at 2,295,043 to 2,295,666
ccmA
b2202: napC - nitrate reductase, cytochrome c-type, periplasmic (NCBI), at 2,295,679 to 2,296,281
napC
Position (kb)
2294
2295
2296 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2293.450 kb on + strand, within ccmD at 2293.526 kb on - strand, within ccmD at 2293.757 kb on - strand, within ccmC at 2293.866 kb on - strand, within ccmC at 2293.966 kb on + strand, within ccmC at 2293.966 kb on + strand, within ccmC at 2293.966 kb on + strand, within ccmC at 2293.966 kb on + strand, within ccmC at 2293.966 kb on + strand, within ccmC at 2294.113 kb on + strand, within ccmC at 2294.244 kb on + strand, within ccmC at 2294.244 kb on + strand, within ccmC at 2294.249 kb on + strand, within ccmC at 2294.284 kb on - strand at 2294.342 kb on - strand at 2294.482 kb on - strand, within ccmB at 2294.482 kb on - strand, within ccmB at 2294.559 kb on + strand, within ccmB at 2294.559 kb on + strand, within ccmB at 2294.744 kb on - strand, within ccmB at 2294.744 kb on - strand, within ccmB at 2294.904 kb on - strand, within ccmB at 2294.928 kb on - strand, within ccmB at 2294.999 kb on + strand at 2295.133 kb on + strand, within ccmA at 2295.133 kb on + strand, within ccmA at 2295.152 kb on + strand, within ccmA at 2295.207 kb on + strand, within ccmA at 2295.207 kb on + strand, within ccmA at 2295.285 kb on + strand, within ccmA at 2295.401 kb on + strand, within ccmA at 2295.433 kb on - strand, within ccmA at 2295.476 kb on + strand, within ccmA at 2295.476 kb on + strand, within ccmA at 2295.516 kb on - strand, within ccmA at 2295.575 kb on - strand, within ccmA at 2295.650 kb on + strand at 2295.690 kb on + strand at 2295.722 kb on - strand at 2295.760 kb on + strand, within napC at 2295.760 kb on + strand, within napC at 2295.769 kb on + strand, within napC at 2295.806 kb on + strand, within napC at 2295.880 kb on + strand, within napC at 2295.921 kb on - strand, within napC
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 2,293,450 + ccmD b2198 0.24 +0.4 2,293,526 - ccmD b2198 0.60 -2.1 2,293,757 - ccmC b2199 0.21 +0.2 2,293,866 - ccmC b2199 0.35 +1.2 2,293,966 + ccmC b2199 0.49 -0.2 2,293,966 + ccmC b2199 0.49 -1.6 2,293,966 + ccmC b2199 0.49 +0.2 2,293,966 + ccmC b2199 0.49 -0.3 2,293,966 + ccmC b2199 0.49 +2.5 2,294,113 + ccmC b2199 0.69 +1.2 2,294,244 + ccmC b2199 0.87 +1.2 2,294,244 + ccmC b2199 0.87 -1.4 2,294,249 + ccmC b2199 0.87 +1.2 2,294,284 - +1.1 2,294,342 - -1.3 2,294,482 - ccmB b2200 0.15 +1.2 2,294,482 - ccmB b2200 0.15 -1.6 2,294,559 + ccmB b2200 0.26 +2.0 2,294,559 + ccmB b2200 0.26 -0.1 2,294,744 - ccmB b2200 0.54 -1.1 2,294,744 - ccmB b2200 0.54 -2.6 2,294,904 - ccmB b2200 0.78 -0.5 2,294,928 - ccmB b2200 0.82 -0.4 2,294,999 + +1.5 2,295,133 + ccmA b2201 0.14 +1.5 2,295,133 + ccmA b2201 0.14 -1.4 2,295,152 + ccmA b2201 0.17 +1.2 2,295,207 + ccmA b2201 0.26 +0.9 2,295,207 + ccmA b2201 0.26 +0.3 2,295,285 + ccmA b2201 0.39 -0.5 2,295,401 + ccmA b2201 0.57 -1.8 2,295,433 - ccmA b2201 0.62 -1.0 2,295,476 + ccmA b2201 0.69 +0.9 2,295,476 + ccmA b2201 0.69 -1.4 2,295,516 - ccmA b2201 0.76 -1.8 2,295,575 - ccmA b2201 0.85 +1.6 2,295,650 + -0.2 2,295,690 + +0.6 2,295,722 - +2.1 2,295,760 + napC b2202 0.13 +2.9 2,295,760 + napC b2202 0.13 +0.9 2,295,769 + napC b2202 0.15 -1.1 2,295,806 + napC b2202 0.21 -0.4 2,295,880 + napC b2202 0.33 +0.7 2,295,921 - napC b2202 0.40 -1.8
Or see this region's nucleotide sequence