Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yejF and yejG are separated by 3 nucleotides yejG and bcr are separated by 332 nucleotides bcr and rsuA are separated by 27 nucleotides rsuA and yejH are separated by 148 nucleotides
b2180: yejF - fused predicted oligopeptide transporter subunits of ABC superfamilly: ATP-binding components (NCBI), at 2,274,322 to 2,275,911
yejF
b2181: yejG - hypothetical protein (NCBI), at 2,275,915 to 2,276,259
yejG
b2182: bcr - bicyclomycin/multidrug efflux system (NCBI), at 2,276,592 to 2,277,782
bcr
b2183: rsuA - 16S rRNA pseudouridylate 516 synthase (NCBI), at 2,277,810 to 2,278,505
rsuA
b2184: yejH - DNA damage response helicase (yejH or radD) (from data) , at 2,278,654 to 2,280,414
yejH
Position (kb)
2276
2277
2278 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2275.673 kb on + strand, within yejF at 2275.814 kb on - strand at 2275.816 kb on - strand at 2275.870 kb on + strand at 2275.870 kb on + strand at 2276.009 kb on + strand, within yejG at 2276.027 kb on - strand, within yejG at 2276.032 kb on + strand, within yejG at 2276.032 kb on + strand, within yejG at 2276.032 kb on + strand, within yejG at 2276.032 kb on - strand, within yejG at 2276.078 kb on + strand, within yejG at 2276.099 kb on + strand, within yejG at 2276.108 kb on - strand, within yejG at 2276.245 kb on - strand at 2276.332 kb on + strand at 2276.374 kb on + strand at 2276.406 kb on - strand at 2276.411 kb on - strand at 2276.411 kb on - strand at 2276.429 kb on - strand at 2276.885 kb on - strand, within bcr at 2276.892 kb on - strand, within bcr at 2276.892 kb on - strand, within bcr at 2276.950 kb on - strand, within bcr at 2276.950 kb on - strand, within bcr at 2277.132 kb on + strand, within bcr at 2277.137 kb on + strand, within bcr at 2277.197 kb on + strand, within bcr at 2277.287 kb on + strand, within bcr at 2277.287 kb on + strand, within bcr at 2277.352 kb on - strand, within bcr at 2277.352 kb on - strand, within bcr at 2277.352 kb on - strand, within bcr at 2277.422 kb on - strand, within bcr at 2277.445 kb on + strand, within bcr at 2277.573 kb on + strand, within bcr at 2277.625 kb on - strand, within bcr at 2277.628 kb on - strand, within bcr at 2277.641 kb on + strand, within bcr at 2277.695 kb on + strand at 2277.695 kb on + strand at 2277.696 kb on + strand at 2277.759 kb on + strand at 2277.767 kb on + strand at 2278.002 kb on - strand, within rsuA at 2278.018 kb on - strand, within rsuA at 2278.126 kb on - strand, within rsuA at 2278.173 kb on + strand, within rsuA at 2278.266 kb on - strand, within rsuA at 2278.285 kb on - strand, within rsuA at 2278.462 kb on - strand at 2278.560 kb on - strand at 2278.678 kb on - strand at 2278.720 kb on + strand at 2278.720 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 2,275,673 + yejF b2180 0.85 -0.5 2,275,814 - -1.0 2,275,816 - -0.2 2,275,870 + -0.8 2,275,870 + +0.3 2,276,009 + yejG b2181 0.27 +0.9 2,276,027 - yejG b2181 0.32 -0.4 2,276,032 + yejG b2181 0.34 +0.3 2,276,032 + yejG b2181 0.34 -1.1 2,276,032 + yejG b2181 0.34 +0.2 2,276,032 - yejG b2181 0.34 -0.1 2,276,078 + yejG b2181 0.47 -1.0 2,276,099 + yejG b2181 0.53 -0.1 2,276,108 - yejG b2181 0.56 -1.1 2,276,245 - +0.2 2,276,332 + -1.6 2,276,374 + +0.4 2,276,406 - +4.2 2,276,411 - +0.4 2,276,411 - +2.6 2,276,429 - +2.6 2,276,885 - bcr b2182 0.25 -1.1 2,276,892 - bcr b2182 0.25 -1.6 2,276,892 - bcr b2182 0.25 -2.6 2,276,950 - bcr b2182 0.30 -0.4 2,276,950 - bcr b2182 0.30 -0.4 2,277,132 + bcr b2182 0.45 -1.1 2,277,137 + bcr b2182 0.46 -0.9 2,277,197 + bcr b2182 0.51 -1.1 2,277,287 + bcr b2182 0.58 -0.8 2,277,287 + bcr b2182 0.58 -0.8 2,277,352 - bcr b2182 0.64 -2.0 2,277,352 - bcr b2182 0.64 +0.2 2,277,352 - bcr b2182 0.64 -0.1 2,277,422 - bcr b2182 0.70 -0.1 2,277,445 + bcr b2182 0.72 -0.1 2,277,573 + bcr b2182 0.82 +1.9 2,277,625 - bcr b2182 0.87 -0.6 2,277,628 - bcr b2182 0.87 -2.0 2,277,641 + bcr b2182 0.88 +1.5 2,277,695 + -0.6 2,277,695 + +0.1 2,277,696 + +1.6 2,277,759 + +0.7 2,277,767 + +0.7 2,278,002 - rsuA b2183 0.28 -2.5 2,278,018 - rsuA b2183 0.30 +0.8 2,278,126 - rsuA b2183 0.45 +1.8 2,278,173 + rsuA b2183 0.52 -1.6 2,278,266 - rsuA b2183 0.66 -0.2 2,278,285 - rsuA b2183 0.68 -0.1 2,278,462 - +0.6 2,278,560 - +1.5 2,278,678 - -0.4 2,278,720 + -0.8 2,278,720 + -0.8
Or see this region's nucleotide sequence