Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yeiH and nfo are separated by 73 nucleotides nfo and yeiI are separated by 2 nucleotides yeiI and yeiJ are separated by 106 nucleotides
b2158: yeiH - conserved inner membrane protein (NCBI), at 2,247,739 to 2,248,788
yeiH
b2159: nfo - endonuclease IV (NCBI), at 2,248,862 to 2,249,719
nfo
b2160: yeiI - predicted kinase (NCBI), at 2,249,722 to 2,250,810
yeiI
b2161: yeiJ - predicted nucleoside transporter (NCBI), at 2,250,917 to 2,252,167
yeiJ
Position (kb)
2249
2250
2251 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2248.723 kb on - strand at 2248.805 kb on - strand at 2248.805 kb on - strand at 2248.869 kb on + strand at 2248.877 kb on + strand at 2248.882 kb on + strand at 2248.890 kb on - strand at 2248.998 kb on - strand, within nfo at 2249.049 kb on - strand, within nfo at 2249.301 kb on + strand, within nfo at 2249.311 kb on + strand, within nfo at 2249.311 kb on + strand, within nfo at 2249.408 kb on + strand, within nfo at 2249.543 kb on + strand, within nfo at 2249.543 kb on + strand, within nfo at 2249.580 kb on + strand, within nfo at 2249.588 kb on + strand, within nfo at 2249.601 kb on - strand, within nfo at 2249.859 kb on + strand, within yeiI at 2249.900 kb on - strand, within yeiI at 2249.900 kb on - strand, within yeiI at 2250.009 kb on + strand, within yeiI at 2250.009 kb on + strand, within yeiI at 2250.121 kb on - strand, within yeiI at 2250.126 kb on - strand, within yeiI at 2250.314 kb on - strand, within yeiI at 2250.314 kb on - strand, within yeiI at 2250.332 kb on - strand, within yeiI at 2250.333 kb on + strand, within yeiI at 2250.341 kb on - strand, within yeiI at 2250.500 kb on - strand, within yeiI at 2250.620 kb on + strand, within yeiI at 2250.620 kb on + strand, within yeiI at 2250.640 kb on - strand, within yeiI at 2250.640 kb on - strand, within yeiI at 2250.667 kb on + strand, within yeiI at 2250.761 kb on + strand at 2250.761 kb on + strand at 2250.761 kb on + strand at 2250.761 kb on + strand at 2250.769 kb on - strand at 2250.769 kb on - strand at 2250.916 kb on - strand at 2250.937 kb on + strand at 2250.937 kb on + strand at 2251.136 kb on - strand, within yeiJ at 2251.247 kb on - strand, within yeiJ at 2251.251 kb on - strand, within yeiJ at 2251.503 kb on + strand, within yeiJ at 2251.550 kb on - strand, within yeiJ at 2251.550 kb on - strand, within yeiJ at 2251.583 kb on - strand, within yeiJ at 2251.627 kb on + strand, within yeiJ at 2251.627 kb on + strand, within yeiJ at 2251.736 kb on + strand, within yeiJ at 2251.736 kb on + strand, within yeiJ
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 2,248,723 - +1.9 2,248,805 - +1.3 2,248,805 - +2.4 2,248,869 + -1.1 2,248,877 + +2.5 2,248,882 + +0.4 2,248,890 - -2.0 2,248,998 - nfo b2159 0.16 +1.2 2,249,049 - nfo b2159 0.22 -2.5 2,249,301 + nfo b2159 0.51 +2.5 2,249,311 + nfo b2159 0.52 -0.9 2,249,311 + nfo b2159 0.52 +0.2 2,249,408 + nfo b2159 0.64 -2.8 2,249,543 + nfo b2159 0.79 -0.0 2,249,543 + nfo b2159 0.79 +1.2 2,249,580 + nfo b2159 0.84 -0.6 2,249,588 + nfo b2159 0.85 +0.8 2,249,601 - nfo b2159 0.86 +0.2 2,249,859 + yeiI b2160 0.13 +0.0 2,249,900 - yeiI b2160 0.16 +0.3 2,249,900 - yeiI b2160 0.16 +0.6 2,250,009 + yeiI b2160 0.26 +2.2 2,250,009 + yeiI b2160 0.26 -1.6 2,250,121 - yeiI b2160 0.37 -3.3 2,250,126 - yeiI b2160 0.37 +1.7 2,250,314 - yeiI b2160 0.54 +1.9 2,250,314 - yeiI b2160 0.54 +1.4 2,250,332 - yeiI b2160 0.56 -2.0 2,250,333 + yeiI b2160 0.56 +0.3 2,250,341 - yeiI b2160 0.57 -0.7 2,250,500 - yeiI b2160 0.71 +1.7 2,250,620 + yeiI b2160 0.82 -0.6 2,250,620 + yeiI b2160 0.82 +1.2 2,250,640 - yeiI b2160 0.84 +2.2 2,250,640 - yeiI b2160 0.84 -2.1 2,250,667 + yeiI b2160 0.87 +0.7 2,250,761 + +0.2 2,250,761 + +0.1 2,250,761 + +0.2 2,250,761 + +3.4 2,250,769 - -0.8 2,250,769 - +0.2 2,250,916 - -0.4 2,250,937 + +2.2 2,250,937 + -0.6 2,251,136 - yeiJ b2161 0.18 +1.1 2,251,247 - yeiJ b2161 0.26 +1.0 2,251,251 - yeiJ b2161 0.27 -0.0 2,251,503 + yeiJ b2161 0.47 +1.0 2,251,550 - yeiJ b2161 0.51 +0.9 2,251,550 - yeiJ b2161 0.51 -0.4 2,251,583 - yeiJ b2161 0.53 +0.6 2,251,627 + yeiJ b2161 0.57 +1.2 2,251,627 + yeiJ b2161 0.57 -0.8 2,251,736 + yeiJ b2161 0.65 -0.1 2,251,736 + yeiJ b2161 0.65 -0.4
Or see this region's nucleotide sequence