Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yegU and yegV overlap by 4 nucleotides yegV and yegW overlap by 27 nucleotides yegW and yegX are separated by 51 nucleotides
b2098: yegT - predicted nucleoside transporter (NCBI), at 2,176,843 to 2,178,120
yegT
b2099: yegU - predicted hydrolase (NCBI), at 2,178,117 to 2,179,121
yegU
b2100: yegV - predicted kinase (NCBI), at 2,179,118 to 2,180,083
yegV
b2101: yegW - predicted DNA-binding transcriptional regulator (NCBI), at 2,180,057 to 2,180,803
yegW
b2102: yegX - orf, hypothetical protein (VIMSS), at 2,180,855 to 2,181,673
yegX
Position (kb)
2179
2180
2181 Strain fitness (log2 ratio)
-1
0
1
2 at 2178.144 kb on + strand at 2178.181 kb on + strand at 2178.299 kb on + strand, within yegU at 2178.381 kb on + strand, within yegU at 2178.588 kb on - strand, within yegU at 2178.600 kb on - strand, within yegU at 2178.717 kb on - strand, within yegU at 2178.859 kb on + strand, within yegU at 2178.893 kb on + strand, within yegU at 2178.893 kb on + strand, within yegU at 2178.920 kb on + strand, within yegU at 2179.014 kb on - strand, within yegU at 2179.214 kb on + strand at 2179.221 kb on - strand, within yegV at 2179.222 kb on - strand, within yegV at 2179.310 kb on + strand, within yegV at 2179.396 kb on - strand, within yegV at 2179.448 kb on - strand, within yegV at 2179.906 kb on - strand, within yegV at 2179.906 kb on - strand, within yegV at 2179.993 kb on - strand at 2180.103 kb on + strand at 2180.234 kb on + strand, within yegW at 2180.502 kb on - strand, within yegW at 2180.515 kb on + strand, within yegW at 2180.595 kb on - strand, within yegW at 2180.604 kb on + strand, within yegW at 2180.604 kb on + strand, within yegW at 2180.686 kb on - strand, within yegW at 2180.686 kb on - strand, within yegW at 2180.686 kb on - strand, within yegW at 2180.829 kb on + strand at 2180.837 kb on - strand at 2180.886 kb on + strand at 2180.896 kb on - strand at 2180.902 kb on - strand at 2180.902 kb on - strand at 2180.904 kb on - strand at 2180.917 kb on - strand at 2180.934 kb on + strand at 2180.934 kb on + strand at 2180.961 kb on - strand, within yegX at 2181.005 kb on - strand at 2181.005 kb on - strand, within yegX at 2181.027 kb on + strand, within yegX at 2181.027 kb on + strand, within yegX
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 2,178,144 + -1.2 2,178,181 + -0.6 2,178,299 + yegU b2099 0.18 -1.1 2,178,381 + yegU b2099 0.26 -0.5 2,178,588 - yegU b2099 0.47 +0.3 2,178,600 - yegU b2099 0.48 +1.2 2,178,717 - yegU b2099 0.60 +1.5 2,178,859 + yegU b2099 0.74 -0.1 2,178,893 + yegU b2099 0.77 -1.1 2,178,893 + yegU b2099 0.77 +0.7 2,178,920 + yegU b2099 0.80 +0.4 2,179,014 - yegU b2099 0.89 +0.0 2,179,214 + +0.7 2,179,221 - yegV b2100 0.11 -0.8 2,179,222 - yegV b2100 0.11 +1.9 2,179,310 + yegV b2100 0.20 -1.1 2,179,396 - yegV b2100 0.29 +1.9 2,179,448 - yegV b2100 0.34 -0.5 2,179,906 - yegV b2100 0.82 +1.2 2,179,906 - yegV b2100 0.82 +0.6 2,179,993 - -1.1 2,180,103 + +0.3 2,180,234 + yegW b2101 0.24 +2.6 2,180,502 - yegW b2101 0.60 +0.5 2,180,515 + yegW b2101 0.61 +1.0 2,180,595 - yegW b2101 0.72 -1.0 2,180,604 + yegW b2101 0.73 -0.8 2,180,604 + yegW b2101 0.73 -0.4 2,180,686 - yegW b2101 0.84 +1.9 2,180,686 - yegW b2101 0.84 +1.7 2,180,686 - yegW b2101 0.84 -1.1 2,180,829 + +0.8 2,180,837 - -0.2 2,180,886 + -0.6 2,180,896 - +0.5 2,180,902 - -0.0 2,180,902 - -1.4 2,180,904 - +1.5 2,180,917 - -1.5 2,180,934 + +1.2 2,180,934 + +0.2 2,180,961 - yegX b2102 0.13 +2.5 2,181,005 - +1.2 2,181,005 - yegX b2102 0.18 +2.2 2,181,027 + yegX b2102 0.21 +2.2 2,181,027 + yegX b2102 0.21 +0.6
Or see this region's nucleotide sequence