Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt b2060 and wzb are separated by 2 nucleotides wzb and wza are separated by 5 nucleotides wza and yegH are separated by 658 nucleotides
b2060: b2060 - orf, hypothetical protein (VIMSS), at 2,131,514 to 2,133,676
b2060
b2061: wzb - protein-tyrosine phosphatase (NCBI), at 2,133,679 to 2,134,122
wzb
b2062: wza - lipoprotein required for capsular polysaccharide translocation through the outer membrane (NCBI), at 2,134,128 to 2,135,267
wza
b2063: yegH - putative transport protein (VIMSS), at 2,135,926 to 2,137,509
yegH
Position (kb)
2134
2135
2136 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2133.348 kb on - strand, within b2060 at 2133.494 kb on - strand at 2133.538 kb on - strand at 2133.685 kb on - strand at 2134.043 kb on + strand, within wzb at 2134.142 kb on - strand at 2134.142 kb on - strand at 2134.157 kb on - strand at 2134.208 kb on - strand at 2134.244 kb on - strand, within wza at 2134.244 kb on - strand, within wza at 2134.329 kb on + strand, within wza at 2134.385 kb on + strand, within wza at 2134.409 kb on + strand, within wza at 2134.417 kb on - strand, within wza at 2134.417 kb on - strand, within wza at 2134.427 kb on - strand, within wza at 2134.427 kb on - strand, within wza at 2134.545 kb on + strand, within wza at 2134.545 kb on + strand, within wza at 2134.545 kb on + strand, within wza at 2134.599 kb on + strand, within wza at 2134.604 kb on + strand, within wza at 2134.744 kb on + strand, within wza at 2134.801 kb on - strand, within wza at 2134.801 kb on - strand, within wza at 2134.961 kb on + strand, within wza at 2134.991 kb on - strand, within wza at 2134.993 kb on - strand, within wza at 2134.993 kb on - strand, within wza at 2135.044 kb on + strand, within wza at 2135.052 kb on - strand, within wza at 2135.052 kb on - strand, within wza at 2135.055 kb on - strand, within wza at 2135.055 kb on - strand, within wza at 2135.058 kb on + strand, within wza at 2135.072 kb on - strand, within wza at 2135.075 kb on + strand, within wza at 2135.082 kb on - strand, within wza at 2135.082 kb on - strand, within wza at 2135.093 kb on + strand, within wza at 2135.106 kb on + strand, within wza at 2135.114 kb on - strand, within wza at 2135.116 kb on - strand, within wza at 2135.137 kb on + strand, within wza at 2135.176 kb on + strand at 2135.190 kb on + strand at 2135.193 kb on + strand at 2135.195 kb on + strand at 2135.195 kb on + strand at 2135.219 kb on + strand at 2135.421 kb on - strand at 2135.441 kb on - strand at 2135.450 kb on + strand at 2135.904 kb on - strand at 2135.920 kb on + strand at 2135.932 kb on - strand at 2135.944 kb on + strand at 2135.945 kb on + strand at 2135.945 kb on + strand at 2135.954 kb on + strand at 2136.074 kb on - strand at 2136.150 kb on + strand, within yegH at 2136.150 kb on + strand, within yegH at 2136.150 kb on + strand, within yegH at 2136.257 kb on + strand, within yegH at 2136.257 kb on + strand, within yegH
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 2,133,348 - b2060 b2060 0.85 -1.4 2,133,494 - +0.2 2,133,538 - -0.4 2,133,685 - -0.4 2,134,043 + wzb b2061 0.82 -0.8 2,134,142 - +1.2 2,134,142 - -0.4 2,134,157 - -0.1 2,134,208 - -1.5 2,134,244 - wza b2062 0.10 +0.8 2,134,244 - wza b2062 0.10 -0.1 2,134,329 + wza b2062 0.18 -2.5 2,134,385 + wza b2062 0.23 +1.0 2,134,409 + wza b2062 0.25 -1.0 2,134,417 - wza b2062 0.25 -0.5 2,134,417 - wza b2062 0.25 -1.5 2,134,427 - wza b2062 0.26 -0.4 2,134,427 - wza b2062 0.26 +0.6 2,134,545 + wza b2062 0.37 -0.8 2,134,545 + wza b2062 0.37 -1.8 2,134,545 + wza b2062 0.37 +0.6 2,134,599 + wza b2062 0.41 +2.0 2,134,604 + wza b2062 0.42 -0.8 2,134,744 + wza b2062 0.54 -1.0 2,134,801 - wza b2062 0.59 -0.1 2,134,801 - wza b2062 0.59 -1.4 2,134,961 + wza b2062 0.73 -2.3 2,134,991 - wza b2062 0.76 -0.7 2,134,993 - wza b2062 0.76 +0.3 2,134,993 - wza b2062 0.76 +1.2 2,135,044 + wza b2062 0.80 -1.4 2,135,052 - wza b2062 0.81 +1.2 2,135,052 - wza b2062 0.81 -0.8 2,135,055 - wza b2062 0.81 +1.9 2,135,055 - wza b2062 0.81 -1.8 2,135,058 + wza b2062 0.82 +0.8 2,135,072 - wza b2062 0.83 +0.2 2,135,075 + wza b2062 0.83 +1.9 2,135,082 - wza b2062 0.84 -2.0 2,135,082 - wza b2062 0.84 +0.2 2,135,093 + wza b2062 0.85 +0.2 2,135,106 + wza b2062 0.86 +0.5 2,135,114 - wza b2062 0.86 -0.2 2,135,116 - wza b2062 0.87 -0.9 2,135,137 + wza b2062 0.89 +0.2 2,135,176 + +0.6 2,135,190 + +1.0 2,135,193 + -2.7 2,135,195 + +1.6 2,135,195 + -1.4 2,135,219 + +0.6 2,135,421 - -2.1 2,135,441 - +0.2 2,135,450 + +0.5 2,135,904 - +3.4 2,135,920 + +0.2 2,135,932 - +1.7 2,135,944 + -0.4 2,135,945 + +2.3 2,135,945 + +0.4 2,135,954 + +1.7 2,136,074 - +1.6 2,136,150 + yegH b2063 0.14 -0.1 2,136,150 + yegH b2063 0.14 -1.1 2,136,150 + yegH b2063 0.14 -2.0 2,136,257 + yegH b2063 0.21 +1.4 2,136,257 + yegH b2063 0.21 +0.9
Or see this region's nucleotide sequence