Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cpsB and wcaI are separated by 2 nucleotides wcaI and wcaH overlap by 4 nucleotides wcaH and fcl are separated by 2 nucleotides
b2049: cpsB - mannose-1-phosphate guanyltransferase (NCBI), at 2,121,108 to 2,122,544
cpsB
b2050: wcaI - predicted glycosyl transferase (NCBI), at 2,122,547 to 2,123,770
wcaI
b2051: wcaH - GDP-mannose mannosyl hydrolase (VIMSS), at 2,123,767 to 2,124,246
wcaH
b2052: fcl - bifunctional GDP-fucose synthetase: GDP-4-dehydro-6-deoxy-D-mannose epimerase/ GDP-4-dehydro-6-L-deoxygalactose reductase (NCBI), at 2,124,249 to 2,125,214
fcl
Position (kb)
2122
2123
2124 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2121.605 kb on + strand, within cpsB at 2121.742 kb on + strand, within cpsB at 2121.742 kb on + strand, within cpsB at 2121.969 kb on + strand, within cpsB at 2122.061 kb on + strand, within cpsB at 2122.061 kb on + strand, within cpsB at 2122.748 kb on + strand, within wcaI at 2122.748 kb on + strand, within wcaI at 2123.290 kb on + strand, within wcaI at 2123.290 kb on + strand, within wcaI at 2123.479 kb on + strand, within wcaI at 2123.610 kb on + strand, within wcaI at 2123.668 kb on + strand at 2123.822 kb on + strand, within wcaH at 2124.177 kb on - strand, within wcaH at 2124.282 kb on + strand at 2124.282 kb on + strand at 2124.465 kb on + strand, within fcl at 2124.601 kb on + strand, within fcl at 2124.630 kb on + strand, within fcl at 2124.630 kb on + strand, within fcl at 2124.722 kb on - strand, within fcl
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 2,121,605 + cpsB b2049 0.35 -0.1 2,121,742 + cpsB b2049 0.44 +0.2 2,121,742 + cpsB b2049 0.44 +2.2 2,121,969 + cpsB b2049 0.60 -1.6 2,122,061 + cpsB b2049 0.66 -0.4 2,122,061 + cpsB b2049 0.66 +3.5 2,122,748 + wcaI b2050 0.16 -1.2 2,122,748 + wcaI b2050 0.16 -2.0 2,123,290 + wcaI b2050 0.61 -1.1 2,123,290 + wcaI b2050 0.61 -1.1 2,123,479 + wcaI b2050 0.76 +0.3 2,123,610 + wcaI b2050 0.87 +0.2 2,123,668 + +0.8 2,123,822 + wcaH b2051 0.11 -0.4 2,124,177 - wcaH b2051 0.85 -0.4 2,124,282 + +1.5 2,124,282 + +0.5 2,124,465 + fcl b2052 0.22 -0.8 2,124,601 + fcl b2052 0.36 -1.0 2,124,630 + fcl b2052 0.39 +0.2 2,124,630 + fcl b2052 0.39 -1.4 2,124,722 - fcl b2052 0.49 -3.1
Or see this region's nucleotide sequence