Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wcaK and wzxC are separated by 275 nucleotides wzxC and wcaJ are separated by 1 nucleotides
b2045: wcaK - predicted pyruvyl transferase (NCBI), at 2,115,148 to 2,116,428
wcaK
b2046: wzxC - colanic acid exporter (NCBI), at 2,116,704 to 2,118,182
wzxC
b2047: wcaJ - predicted UDP-glucose lipid carrier transferase (NCBI), at 2,118,184 to 2,119,578
wcaJ
Position (kb)
2116
2117
2118
2119 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2115.708 kb on + strand, within wcaK at 2115.716 kb on - strand, within wcaK at 2115.754 kb on - strand, within wcaK at 2115.790 kb on + strand, within wcaK at 2115.941 kb on + strand, within wcaK at 2115.941 kb on + strand, within wcaK at 2115.949 kb on - strand, within wcaK at 2115.959 kb on - strand, within wcaK at 2115.959 kb on - strand, within wcaK at 2115.968 kb on + strand, within wcaK at 2115.968 kb on + strand, within wcaK at 2115.976 kb on - strand, within wcaK at 2115.995 kb on + strand, within wcaK at 2116.108 kb on + strand, within wcaK at 2116.108 kb on + strand, within wcaK at 2116.413 kb on + strand at 2116.513 kb on - strand at 2116.513 kb on - strand at 2116.566 kb on - strand at 2116.566 kb on - strand at 2116.604 kb on - strand at 2116.868 kb on + strand, within wzxC at 2116.868 kb on + strand, within wzxC at 2116.880 kb on + strand, within wzxC at 2116.888 kb on - strand, within wzxC at 2116.888 kb on - strand, within wzxC at 2116.925 kb on + strand, within wzxC at 2117.172 kb on - strand, within wzxC at 2117.175 kb on + strand, within wzxC at 2117.196 kb on - strand, within wzxC at 2117.246 kb on + strand, within wzxC at 2117.262 kb on - strand, within wzxC at 2117.262 kb on - strand, within wzxC at 2117.272 kb on + strand, within wzxC at 2117.301 kb on + strand, within wzxC at 2117.431 kb on + strand, within wzxC at 2117.652 kb on - strand, within wzxC at 2117.652 kb on - strand, within wzxC at 2117.721 kb on + strand, within wzxC at 2117.877 kb on + strand, within wzxC at 2117.877 kb on + strand, within wzxC at 2118.014 kb on - strand, within wzxC at 2118.054 kb on - strand at 2118.054 kb on - strand at 2118.126 kb on - strand at 2118.353 kb on + strand, within wcaJ at 2118.353 kb on + strand, within wcaJ at 2118.391 kb on - strand, within wcaJ at 2118.476 kb on - strand, within wcaJ at 2119.085 kb on - strand, within wcaJ at 2119.106 kb on - strand, within wcaJ
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 2,115,708 + wcaK b2045 0.44 -1.2 2,115,716 - wcaK b2045 0.44 +0.4 2,115,754 - wcaK b2045 0.47 +0.2 2,115,790 + wcaK b2045 0.50 -1.1 2,115,941 + wcaK b2045 0.62 +0.2 2,115,941 + wcaK b2045 0.62 -1.2 2,115,949 - wcaK b2045 0.63 +1.8 2,115,959 - wcaK b2045 0.63 -0.6 2,115,959 - wcaK b2045 0.63 -2.3 2,115,968 + wcaK b2045 0.64 -1.8 2,115,968 + wcaK b2045 0.64 +3.1 2,115,976 - wcaK b2045 0.65 +2.2 2,115,995 + wcaK b2045 0.66 -0.4 2,116,108 + wcaK b2045 0.75 +3.0 2,116,108 + wcaK b2045 0.75 -0.2 2,116,413 + +0.7 2,116,513 - +0.7 2,116,513 - -1.1 2,116,566 - +1.2 2,116,566 - -0.7 2,116,604 - +0.6 2,116,868 + wzxC b2046 0.11 +0.9 2,116,868 + wzxC b2046 0.11 +0.6 2,116,880 + wzxC b2046 0.12 -0.3 2,116,888 - wzxC b2046 0.12 -1.6 2,116,888 - wzxC b2046 0.12 -0.2 2,116,925 + wzxC b2046 0.15 -1.2 2,117,172 - wzxC b2046 0.32 +0.2 2,117,175 + wzxC b2046 0.32 +2.8 2,117,196 - wzxC b2046 0.33 -0.9 2,117,246 + wzxC b2046 0.37 -0.0 2,117,262 - wzxC b2046 0.38 -0.8 2,117,262 - wzxC b2046 0.38 +0.6 2,117,272 + wzxC b2046 0.38 -0.6 2,117,301 + wzxC b2046 0.40 -0.2 2,117,431 + wzxC b2046 0.49 -0.3 2,117,652 - wzxC b2046 0.64 +0.2 2,117,652 - wzxC b2046 0.64 -1.5 2,117,721 + wzxC b2046 0.69 -1.2 2,117,877 + wzxC b2046 0.79 +0.8 2,117,877 + wzxC b2046 0.79 -0.7 2,118,014 - wzxC b2046 0.89 -2.1 2,118,054 - -0.8 2,118,054 - +0.2 2,118,126 - +0.2 2,118,353 + wcaJ b2047 0.12 +0.8 2,118,353 + wcaJ b2047 0.12 -0.6 2,118,391 - wcaJ b2047 0.15 +1.3 2,118,476 - wcaJ b2047 0.21 +0.6 2,119,085 - wcaJ b2047 0.65 +0.8 2,119,106 - wcaJ b2047 0.66 -0.8
Or see this region's nucleotide sequence