Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dcm and yedJ are separated by 66 nucleotides yedJ and b1963 are separated by 39 nucleotides b1963 and rseX are separated by 164 nucleotides rseX and yedS are separated by 311 nucleotides
b1961: dcm - DNA cytosine methylase (NCBI), at 2,028,923 to 2,030,341
dcm
b1962: yedJ - predicted phosphohydrolase (NCBI), at 2,030,408 to 2,031,103
yedJ
b1963: b1963 - orf, hypothetical protein (VIMSS), at 2,031,143 to 2,031,508
b1963
b4603: rseX - sRNA regulating ompA and ompC translation, with Hfq (RefSeq), at 2,031,673 to 2,031,763
rseX
b4496: yedS - no description, at 2,032,075 to 2,033,267
yedS
Position (kb)
2030
2031
2032 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2030.164 kb on + strand, within dcm at 2030.226 kb on + strand at 2030.226 kb on + strand at 2030.231 kb on + strand at 2030.451 kb on + strand at 2030.564 kb on + strand, within yedJ at 2030.595 kb on + strand, within yedJ at 2030.690 kb on + strand, within yedJ at 2030.849 kb on + strand, within yedJ at 2031.000 kb on + strand, within yedJ at 2031.033 kb on - strand, within yedJ at 2031.117 kb on + strand at 2031.157 kb on + strand at 2031.157 kb on + strand at 2031.174 kb on + strand at 2031.179 kb on + strand at 2031.179 kb on + strand at 2031.181 kb on - strand, within b1963 at 2031.181 kb on - strand, within b1963 at 2031.226 kb on + strand, within b1963 at 2031.226 kb on + strand, within b1963 at 2031.244 kb on + strand, within b1963 at 2031.369 kb on - strand, within b1963 at 2031.378 kb on + strand, within b1963 at 2031.473 kb on + strand at 2031.507 kb on + strand at 2031.521 kb on + strand at 2031.522 kb on + strand at 2031.527 kb on - strand at 2031.527 kb on - strand at 2031.529 kb on - strand at 2031.697 kb on + strand, within rseX at 2031.776 kb on - strand at 2031.995 kb on - strand at 2031.995 kb on - strand at 2032.073 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 2,030,164 + dcm b1961 0.87 -0.5 2,030,226 + -1.4 2,030,226 + +1.7 2,030,231 + -1.4 2,030,451 + -0.1 2,030,564 + yedJ b1962 0.22 +1.0 2,030,595 + yedJ b1962 0.27 -1.8 2,030,690 + yedJ b1962 0.41 -2.1 2,030,849 + yedJ b1962 0.63 -2.2 2,031,000 + yedJ b1962 0.85 +0.9 2,031,033 - yedJ b1962 0.90 -0.9 2,031,117 + -2.4 2,031,157 + +1.8 2,031,157 + +0.2 2,031,174 + -1.0 2,031,179 + -1.8 2,031,179 + +0.6 2,031,181 - b1963 b1963 0.10 -1.6 2,031,181 - b1963 b1963 0.10 -0.4 2,031,226 + b1963 b1963 0.23 +3.3 2,031,226 + b1963 b1963 0.23 +1.6 2,031,244 + b1963 b1963 0.28 +1.2 2,031,369 - b1963 b1963 0.62 -1.0 2,031,378 + b1963 b1963 0.64 +1.2 2,031,473 + -0.5 2,031,507 + -2.4 2,031,521 + +0.9 2,031,522 + -1.6 2,031,527 - -0.4 2,031,527 - -0.6 2,031,529 - -0.0 2,031,697 + rseX b4603 0.26 +1.0 2,031,776 - -0.2 2,031,995 - +2.5 2,031,995 - -0.6 2,032,073 - +1.5
Or see this region's nucleotide sequence