Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aspS and yecD are separated by 309 nucleotides yecD and yecE overlap by 4 nucleotides yecE and yecN are separated by 52 nucleotides yecN and yecO are separated by 40 nucleotides
b1866: aspS - aspartyl-tRNA synthetase (NCBI), at 1,946,774 to 1,948,546
aspS
b1867: yecD - orf, hypothetical protein (VIMSS), at 1,948,856 to 1,949,422
yecD
b1868: yecE - hypothetical protein (NCBI), at 1,949,419 to 1,950,237
yecE
b1869: yecN - orf, hypothetical protein (VIMSS), at 1,950,290 to 1,950,685
yecN
b1870: yecO - predicted methyltransferase (NCBI), at 1,950,726 to 1,951,469
yecO
Position (kb)
1949
1950
1951 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1948.556 kb on - strand at 1948.718 kb on - strand at 1948.761 kb on + strand at 1948.771 kb on + strand at 1948.859 kb on - strand at 1948.859 kb on - strand at 1948.932 kb on + strand, within yecD at 1949.048 kb on + strand, within yecD at 1949.173 kb on + strand, within yecD at 1949.173 kb on + strand, within yecD at 1949.179 kb on - strand, within yecD at 1949.186 kb on + strand, within yecD at 1949.186 kb on + strand, within yecD at 1949.189 kb on - strand, within yecD at 1949.228 kb on + strand, within yecD at 1949.228 kb on + strand, within yecD at 1949.228 kb on - strand, within yecD at 1949.228 kb on - strand, within yecD at 1949.231 kb on - strand, within yecD at 1949.349 kb on + strand, within yecD at 1949.404 kb on - strand at 1949.404 kb on - strand at 1949.442 kb on - strand at 1949.709 kb on + strand, within yecE at 1949.709 kb on + strand, within yecE at 1949.746 kb on - strand, within yecE at 1949.842 kb on - strand, within yecE at 1949.842 kb on - strand, within yecE at 1949.931 kb on + strand, within yecE at 1950.249 kb on + strand at 1950.310 kb on - strand at 1950.444 kb on + strand, within yecN at 1950.475 kb on + strand, within yecN at 1950.475 kb on + strand, within yecN at 1950.509 kb on + strand, within yecN at 1950.517 kb on - strand, within yecN at 1950.525 kb on + strand, within yecN at 1950.525 kb on + strand, within yecN at 1950.612 kb on + strand, within yecN at 1950.612 kb on + strand, within yecN at 1950.613 kb on - strand, within yecN at 1950.613 kb on - strand, within yecN at 1950.743 kb on + strand at 1951.029 kb on + strand, within yecO
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 1,948,556 - -1.8 1,948,718 - +1.3 1,948,761 + -1.3 1,948,771 + +3.7 1,948,859 - +1.5 1,948,859 - +0.2 1,948,932 + yecD b1867 0.13 -0.9 1,949,048 + yecD b1867 0.34 +0.9 1,949,173 + yecD b1867 0.56 -1.6 1,949,173 + yecD b1867 0.56 +1.5 1,949,179 - yecD b1867 0.57 -0.2 1,949,186 + yecD b1867 0.58 +1.8 1,949,186 + yecD b1867 0.58 -0.6 1,949,189 - yecD b1867 0.59 -0.7 1,949,228 + yecD b1867 0.66 -1.8 1,949,228 + yecD b1867 0.66 -2.2 1,949,228 - yecD b1867 0.66 +2.5 1,949,228 - yecD b1867 0.66 -0.5 1,949,231 - yecD b1867 0.66 -0.1 1,949,349 + yecD b1867 0.87 +0.2 1,949,404 - -0.9 1,949,404 - -1.1 1,949,442 - -1.7 1,949,709 + yecE b1868 0.35 -1.1 1,949,709 + yecE b1868 0.35 -0.8 1,949,746 - yecE b1868 0.40 -0.3 1,949,842 - yecE b1868 0.52 -1.3 1,949,842 - yecE b1868 0.52 -1.0 1,949,931 + yecE b1868 0.63 +0.1 1,950,249 + +0.3 1,950,310 - -0.2 1,950,444 + yecN b1869 0.39 -0.3 1,950,475 + yecN b1869 0.47 -1.8 1,950,475 + yecN b1869 0.47 -1.1 1,950,509 + yecN b1869 0.55 -0.8 1,950,517 - yecN b1869 0.57 +2.7 1,950,525 + yecN b1869 0.59 +0.2 1,950,525 + yecN b1869 0.59 +0.7 1,950,612 + yecN b1869 0.81 -0.3 1,950,612 + yecN b1869 0.81 +3.0 1,950,613 - yecN b1869 0.82 +0.5 1,950,613 - yecN b1869 0.82 -0.1 1,950,743 + -0.2 1,951,029 + yecO b1870 0.41 +2.2
Or see this region's nucleotide sequence