Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydjG and b1772 are separated by 9 nucleotides b1772 and ydjI are separated by 4 nucleotides ydjI and ydjJ are separated by 20 nucleotides
b1771: ydjG - predicted oxidoreductase (NCBI), at 1,853,015 to 1,853,995
ydjG
b1772: b1772 - putative kinase (VIMSS), at 1,854,005 to 1,854,952
b1772
b1773: ydjI - predicted aldolase (NCBI), at 1,854,957 to 1,855,793
ydjI
b1774: ydjJ - predicted oxidoreductase, Zn-dependent and NAD(P)-binding (NCBI), at 1,855,814 to 1,856,857
ydjJ
Position (kb)
1854
1855 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1853.029 kb on - strand at 1853.029 kb on - strand at 1853.048 kb on - strand at 1853.102 kb on + strand at 1853.110 kb on - strand at 1853.170 kb on + strand, within ydjG at 1853.192 kb on - strand, within ydjG at 1853.368 kb on - strand, within ydjG at 1853.425 kb on - strand, within ydjG at 1853.425 kb on - strand, within ydjG at 1853.448 kb on + strand, within ydjG at 1853.537 kb on - strand, within ydjG at 1853.621 kb on - strand, within ydjG at 1853.621 kb on - strand, within ydjG at 1853.666 kb on - strand, within ydjG at 1853.666 kb on - strand, within ydjG at 1853.751 kb on + strand, within ydjG at 1853.763 kb on - strand, within ydjG at 1853.919 kb on - strand at 1853.937 kb on - strand at 1853.947 kb on - strand at 1854.143 kb on - strand, within b1772 at 1854.143 kb on - strand, within b1772 at 1854.178 kb on - strand, within b1772 at 1854.178 kb on - strand, within b1772 at 1854.223 kb on - strand, within b1772 at 1854.246 kb on - strand, within b1772 at 1854.321 kb on - strand, within b1772 at 1854.389 kb on + strand, within b1772 at 1854.397 kb on - strand, within b1772 at 1854.425 kb on - strand, within b1772 at 1854.425 kb on - strand, within b1772 at 1854.461 kb on - strand, within b1772 at 1854.461 kb on - strand, within b1772 at 1854.550 kb on - strand, within b1772 at 1854.626 kb on + strand, within b1772 at 1854.644 kb on - strand, within b1772 at 1854.644 kb on - strand, within b1772 at 1854.651 kb on + strand, within b1772 at 1854.652 kb on + strand, within b1772 at 1854.670 kb on + strand, within b1772 at 1854.670 kb on + strand, within b1772 at 1854.935 kb on - strand at 1854.935 kb on - strand at 1855.036 kb on - strand at 1855.081 kb on - strand, within ydjI at 1855.087 kb on - strand, within ydjI at 1855.097 kb on - strand, within ydjI at 1855.184 kb on - strand, within ydjI at 1855.333 kb on + strand, within ydjI at 1855.336 kb on + strand, within ydjI at 1855.336 kb on + strand, within ydjI at 1855.341 kb on + strand, within ydjI at 1855.378 kb on + strand, within ydjI at 1855.403 kb on + strand, within ydjI at 1855.411 kb on + strand, within ydjI at 1855.550 kb on + strand, within ydjI at 1855.591 kb on + strand, within ydjI at 1855.593 kb on + strand, within ydjI at 1855.593 kb on + strand, within ydjI at 1855.868 kb on - strand at 1855.880 kb on - strand at 1855.890 kb on - strand at 1855.890 kb on - strand at 1855.907 kb on - strand at 1855.944 kb on - strand, within ydjJ at 1855.944 kb on - strand, within ydjJ
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 1,853,029 - +0.6 1,853,029 - +0.2 1,853,048 - +0.0 1,853,102 + -1.2 1,853,110 - -0.8 1,853,170 + ydjG b1771 0.16 -0.2 1,853,192 - ydjG b1771 0.18 +2.1 1,853,368 - ydjG b1771 0.36 +1.2 1,853,425 - ydjG b1771 0.42 -1.9 1,853,425 - ydjG b1771 0.42 +1.2 1,853,448 + ydjG b1771 0.44 +1.2 1,853,537 - ydjG b1771 0.53 +0.0 1,853,621 - ydjG b1771 0.62 +1.5 1,853,621 - ydjG b1771 0.62 -1.9 1,853,666 - ydjG b1771 0.66 -0.1 1,853,666 - ydjG b1771 0.66 -2.1 1,853,751 + ydjG b1771 0.75 -0.6 1,853,763 - ydjG b1771 0.76 +0.6 1,853,919 - +2.2 1,853,937 - +2.0 1,853,947 - +0.1 1,854,143 - b1772 b1772 0.15 -0.5 1,854,143 - b1772 b1772 0.15 -1.4 1,854,178 - b1772 b1772 0.18 -1.6 1,854,178 - b1772 b1772 0.18 +0.9 1,854,223 - b1772 b1772 0.23 +1.4 1,854,246 - b1772 b1772 0.25 +0.8 1,854,321 - b1772 b1772 0.33 -1.1 1,854,389 + b1772 b1772 0.41 -0.4 1,854,397 - b1772 b1772 0.41 +1.6 1,854,425 - b1772 b1772 0.44 -0.4 1,854,425 - b1772 b1772 0.44 -0.5 1,854,461 - b1772 b1772 0.48 -0.8 1,854,461 - b1772 b1772 0.48 -0.8 1,854,550 - b1772 b1772 0.57 -1.1 1,854,626 + b1772 b1772 0.66 -0.8 1,854,644 - b1772 b1772 0.67 +0.6 1,854,644 - b1772 b1772 0.67 -0.8 1,854,651 + b1772 b1772 0.68 -0.9 1,854,652 + b1772 b1772 0.68 -2.2 1,854,670 + b1772 b1772 0.70 -2.5 1,854,670 + b1772 b1772 0.70 -1.6 1,854,935 - -0.9 1,854,935 - -2.2 1,855,036 - -2.2 1,855,081 - ydjI b1773 0.15 +1.9 1,855,087 - ydjI b1773 0.16 -2.0 1,855,097 - ydjI b1773 0.17 -1.4 1,855,184 - ydjI b1773 0.27 +0.5 1,855,333 + ydjI b1773 0.45 -1.1 1,855,336 + ydjI b1773 0.45 +1.4 1,855,336 + ydjI b1773 0.45 -1.3 1,855,341 + ydjI b1773 0.46 -1.4 1,855,378 + ydjI b1773 0.50 -0.2 1,855,403 + ydjI b1773 0.53 -1.4 1,855,411 + ydjI b1773 0.54 +0.2 1,855,550 + ydjI b1773 0.71 -3.5 1,855,591 + ydjI b1773 0.76 -1.7 1,855,593 + ydjI b1773 0.76 -0.6 1,855,593 + ydjI b1773 0.76 +2.4 1,855,868 - -1.5 1,855,880 - +1.6 1,855,890 - +0.9 1,855,890 - -0.8 1,855,907 - +3.3 1,855,944 - ydjJ b1774 0.12 -1.8 1,855,944 - ydjJ b1774 0.12 +2.8
Or see this region's nucleotide sequence