Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt topB and selD are separated by 4 nucleotides selD and ydjA are separated by 116 nucleotides ydjA and sppA are separated by 160 nucleotides
b1763: topB - DNA topoisomerase III (NCBI), at 1,843,023 to 1,844,984
topB
b1764: selD - selenophosphate synthetase (NCBI), at 1,844,989 to 1,846,032
selD
b1765: ydjA - predicted oxidoreductase (NCBI), at 1,846,149 to 1,846,700
ydjA
b1766: sppA - protease IV (signal peptide peptidase) (NCBI), at 1,846,861 to 1,848,717
sppA
Position (kb)
1844
1845
1846
1847 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1844.351 kb on + strand, within topB at 1844.438 kb on + strand, within topB at 1844.443 kb on + strand, within topB at 1844.600 kb on - strand, within topB at 1844.829 kb on - strand at 1845.230 kb on - strand, within selD at 1845.242 kb on - strand, within selD at 1845.254 kb on - strand, within selD at 1845.300 kb on - strand, within selD at 1845.827 kb on - strand, within selD at 1845.827 kb on - strand, within selD at 1845.858 kb on - strand, within selD at 1845.881 kb on - strand, within selD at 1845.883 kb on - strand, within selD at 1846.260 kb on + strand, within ydjA at 1846.671 kb on - strand at 1846.671 kb on - strand at 1846.826 kb on - strand at 1846.828 kb on + strand at 1846.843 kb on - strand at 1846.849 kb on - strand at 1846.849 kb on - strand at 1846.860 kb on + strand at 1846.860 kb on + strand at 1846.910 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 1,844,351 + topB b1763 0.68 -0.4 1,844,438 + topB b1763 0.72 -1.1 1,844,443 + topB b1763 0.72 -0.6 1,844,600 - topB b1763 0.80 -1.1 1,844,829 - -0.4 1,845,230 - selD b1764 0.23 +1.4 1,845,242 - selD b1764 0.24 +1.6 1,845,254 - selD b1764 0.25 +0.5 1,845,300 - selD b1764 0.30 +2.5 1,845,827 - selD b1764 0.80 +3.0 1,845,827 - selD b1764 0.80 +2.2 1,845,858 - selD b1764 0.83 +2.8 1,845,881 - selD b1764 0.85 +2.8 1,845,883 - selD b1764 0.86 +3.0 1,846,260 + ydjA b1765 0.20 +2.5 1,846,671 - +0.2 1,846,671 - +2.8 1,846,826 - -1.4 1,846,828 + +1.6 1,846,843 - -0.5 1,846,849 - -0.8 1,846,849 - +1.2 1,846,860 + +0.2 1,846,860 + -1.8 1,846,910 - -2.2
Or see this region's nucleotide sequence