Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt xthA and ydjX are separated by 166 nucleotides ydjX and ydjY are separated by 4 nucleotides ydjY and ydjZ are separated by 14 nucleotides
b1749: xthA - exonuclease III (NCBI), at 1,830,452 to 1,831,258
xthA
b1750: ydjX - orf, hypothetical protein (VIMSS), at 1,831,425 to 1,832,135
ydjX
b1751: ydjY - orf, hypothetical protein (VIMSS), at 1,832,140 to 1,832,817
ydjY
b1752: ydjZ - conserved inner membrane protein (NCBI), at 1,832,832 to 1,833,539
ydjZ
Position (kb)
1831
1832
1833 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1830.640 kb on - strand, within xthA at 1830.904 kb on + strand, within xthA at 1830.904 kb on + strand, within xthA at 1830.979 kb on + strand, within xthA at 1831.114 kb on + strand, within xthA at 1831.114 kb on + strand, within xthA at 1831.119 kb on + strand, within xthA at 1831.119 kb on + strand, within xthA at 1831.243 kb on - strand at 1831.243 kb on - strand at 1831.277 kb on - strand at 1831.277 kb on - strand at 1831.351 kb on + strand at 1831.351 kb on + strand at 1831.504 kb on - strand, within ydjX at 1831.535 kb on - strand, within ydjX at 1831.535 kb on - strand, within ydjX at 1831.539 kb on - strand, within ydjX at 1831.642 kb on + strand, within ydjX at 1831.658 kb on + strand, within ydjX at 1831.679 kb on - strand, within ydjX at 1831.737 kb on - strand, within ydjX at 1831.737 kb on - strand, within ydjX at 1831.766 kb on + strand, within ydjX at 1831.766 kb on + strand, within ydjX at 1831.775 kb on + strand, within ydjX at 1831.782 kb on + strand, within ydjX at 1831.827 kb on - strand, within ydjX at 1831.834 kb on + strand, within ydjX at 1831.992 kb on - strand, within ydjX at 1832.016 kb on + strand, within ydjX at 1832.016 kb on + strand, within ydjX at 1832.084 kb on - strand at 1832.113 kb on - strand at 1832.113 kb on - strand at 1832.187 kb on + strand at 1832.403 kb on + strand, within ydjY at 1832.456 kb on + strand, within ydjY at 1832.456 kb on + strand, within ydjY at 1832.456 kb on + strand, within ydjY at 1832.456 kb on + strand, within ydjY at 1832.475 kb on - strand, within ydjY at 1832.475 kb on - strand, within ydjY at 1832.682 kb on + strand, within ydjY at 1832.707 kb on - strand, within ydjY
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 1,830,640 - xthA b1749 0.23 +0.8 1,830,904 + xthA b1749 0.56 +1.2 1,830,904 + xthA b1749 0.56 +0.2 1,830,979 + xthA b1749 0.65 -0.8 1,831,114 + xthA b1749 0.82 -0.4 1,831,114 + xthA b1749 0.82 -1.8 1,831,119 + xthA b1749 0.83 -1.9 1,831,119 + xthA b1749 0.83 -1.1 1,831,243 - +0.0 1,831,243 - -2.2 1,831,277 - +0.6 1,831,277 - -1.6 1,831,351 + +0.2 1,831,351 + +1.2 1,831,504 - ydjX b1750 0.11 +1.8 1,831,535 - ydjX b1750 0.15 +1.0 1,831,535 - ydjX b1750 0.15 -0.8 1,831,539 - ydjX b1750 0.16 +1.2 1,831,642 + ydjX b1750 0.31 +1.6 1,831,658 + ydjX b1750 0.33 +1.3 1,831,679 - ydjX b1750 0.36 +0.2 1,831,737 - ydjX b1750 0.44 +1.1 1,831,737 - ydjX b1750 0.44 +1.5 1,831,766 + ydjX b1750 0.48 -1.2 1,831,766 + ydjX b1750 0.48 +1.2 1,831,775 + ydjX b1750 0.49 +0.8 1,831,782 + ydjX b1750 0.50 -0.8 1,831,827 - ydjX b1750 0.57 -1.1 1,831,834 + ydjX b1750 0.58 +3.0 1,831,992 - ydjX b1750 0.80 +2.7 1,832,016 + ydjX b1750 0.83 +0.2 1,832,016 + ydjX b1750 0.83 +1.4 1,832,084 - +0.4 1,832,113 - +1.5 1,832,113 - +1.2 1,832,187 + -2.1 1,832,403 + ydjY b1751 0.39 +0.1 1,832,456 + ydjY b1751 0.47 -0.4 1,832,456 + ydjY b1751 0.47 +0.2 1,832,456 + ydjY b1751 0.47 +1.6 1,832,456 + ydjY b1751 0.47 -1.1 1,832,475 - ydjY b1751 0.49 -1.8 1,832,475 - ydjY b1751 0.49 +2.5 1,832,682 + ydjY b1751 0.80 +1.7 1,832,707 - ydjY b1751 0.84 +0.8
Or see this region's nucleotide sequence