Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydhT and ydhU are separated by 3 nucleotides ydhU and ydhX overlap by 4 nucleotides ydhX and ydhW are separated by 63 nucleotides ydhW and ydhV are separated by 3 nucleotides
b1669: ydhT - hypothetical protein (NCBI), at 1,746,771 to 1,747,583
ydhT
b1670: ydhU - predicted cytochrome (NCBI), at 1,747,587 to 1,748,372
ydhU
b1671: ydhX - predicted 4Fe-4S ferridoxin-type protein (RefSeq), at 1,748,369 to 1,749,037
ydhX
b1672: ydhW - hypothetical protein (NCBI), at 1,749,101 to 1,749,748
ydhW
b1673: ydhV - predicted oxidoreductase (NCBI), at 1,749,752 to 1,751,854
ydhV
Position (kb)
1748
1749
1750 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1747.895 kb on - strand, within ydhU at 1747.958 kb on - strand, within ydhU at 1747.958 kb on - strand, within ydhU at 1747.965 kb on - strand, within ydhU at 1748.076 kb on - strand, within ydhU at 1748.231 kb on - strand, within ydhU at 1748.231 kb on - strand, within ydhU at 1748.764 kb on - strand, within ydhX at 1748.764 kb on - strand, within ydhX at 1748.847 kb on - strand, within ydhX at 1749.057 kb on - strand at 1749.057 kb on - strand at 1749.123 kb on + strand at 1749.144 kb on + strand at 1749.151 kb on - strand at 1749.425 kb on + strand, within ydhW at 1749.504 kb on - strand, within ydhW at 1749.504 kb on - strand, within ydhW at 1749.504 kb on - strand, within ydhW at 1749.607 kb on + strand, within ydhW at 1749.666 kb on - strand, within ydhW at 1749.802 kb on + strand at 1749.802 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 1,747,895 - ydhU b1670 0.39 -1.0 1,747,958 - ydhU b1670 0.47 -1.1 1,747,958 - ydhU b1670 0.47 -0.4 1,747,965 - ydhU b1670 0.48 +2.6 1,748,076 - ydhU b1670 0.62 +0.6 1,748,231 - ydhU b1670 0.82 -0.1 1,748,231 - ydhU b1670 0.82 -0.2 1,748,764 - ydhX b1671 0.59 +2.2 1,748,764 - ydhX b1671 0.59 +0.2 1,748,847 - ydhX b1671 0.71 +3.0 1,749,057 - -1.4 1,749,057 - +2.2 1,749,123 + +0.1 1,749,144 + +1.2 1,749,151 - +3.4 1,749,425 + ydhW b1672 0.50 +0.4 1,749,504 - ydhW b1672 0.62 +1.2 1,749,504 - ydhW b1672 0.62 -1.4 1,749,504 - ydhW b1672 0.62 +2.2 1,749,607 + ydhW b1672 0.78 -0.4 1,749,666 - ydhW b1672 0.87 +1.5 1,749,802 + -1.1 1,749,802 + +2.0
Or see this region's nucleotide sequence