Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt b1445 and ydcY are separated by 85 nucleotides ydcY and ydcZ are separated by 0 nucleotides ydcZ and yncA overlap by 4 nucleotides yncA and yncB are separated by 180 nucleotides
b1445: b1445 - orf, hypothetical protein (VIMSS), at 1,515,413 to 1,515,586
b1445
b1446: ydcY - hypothetical protein (NCBI), at 1,515,672 to 1,515,905
ydcY
b1447: ydcZ - predicted inner membrane protein (NCBI), at 1,515,906 to 1,516,355
ydcZ
b1448: yncA - predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI), at 1,516,352 to 1,516,870
yncA
b1449: yncB - predicted oxidoreductase, Zn-dependent and NAD(P)-binding (RefSeq), at 1,517,051 to 1,518,088
yncB
Position (kb)
1516
1517 Strain fitness (log2 ratio)
-1
0
1
2 at 1515.608 kb on + strand at 1515.670 kb on - strand at 1515.791 kb on + strand, within ydcY at 1515.839 kb on - strand, within ydcY at 1515.839 kb on - strand, within ydcY at 1515.892 kb on + strand at 1515.892 kb on + strand at 1516.613 kb on - strand, within yncA at 1516.789 kb on - strand, within yncA at 1516.831 kb on + strand at 1516.870 kb on + strand at 1516.878 kb on - strand at 1517.165 kb on + strand, within yncB at 1517.165 kb on + strand, within yncB at 1517.246 kb on - strand, within yncB at 1517.310 kb on + strand, within yncB at 1517.341 kb on + strand, within yncB at 1517.341 kb on + strand, within yncB at 1517.496 kb on - strand, within yncB at 1517.517 kb on - strand, within yncB at 1517.635 kb on - strand, within yncB at 1517.635 kb on - strand, within yncB at 1517.739 kb on - strand, within yncB at 1517.739 kb on - strand, within yncB at 1517.741 kb on - strand, within yncB at 1517.805 kb on + strand, within yncB at 1517.805 kb on + strand, within yncB at 1517.839 kb on - strand, within yncB at 1517.839 kb on - strand, within yncB
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 1,515,608 + +1.6 1,515,670 - +0.9 1,515,791 + ydcY b1446 0.51 -0.9 1,515,839 - ydcY b1446 0.71 -0.9 1,515,839 - ydcY b1446 0.71 -0.4 1,515,892 + +0.7 1,515,892 + +1.6 1,516,613 - yncA b1448 0.50 +0.9 1,516,789 - yncA b1448 0.84 +2.1 1,516,831 + +0.4 1,516,870 + -0.4 1,516,878 - +0.5 1,517,165 + yncB b1449 0.11 +1.9 1,517,165 + yncB b1449 0.11 -0.1 1,517,246 - yncB b1449 0.19 +1.6 1,517,310 + yncB b1449 0.25 -0.2 1,517,341 + yncB b1449 0.28 +0.1 1,517,341 + yncB b1449 0.28 +1.6 1,517,496 - yncB b1449 0.43 +0.6 1,517,517 - yncB b1449 0.45 -0.5 1,517,635 - yncB b1449 0.56 -0.3 1,517,635 - yncB b1449 0.56 -1.2 1,517,739 - yncB b1449 0.66 +1.5 1,517,739 - yncB b1449 0.66 -0.2 1,517,741 - yncB b1449 0.66 +0.1 1,517,805 + yncB b1449 0.73 -0.0 1,517,805 + yncB b1449 0.73 -1.7 1,517,839 - yncB b1449 0.76 +0.1 1,517,839 - yncB b1449 0.76 +0.4
Or see this region's nucleotide sequence