Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydcT and ydcU are separated by 0 nucleotides ydcU and ydcV overlap by 11 nucleotides ydcV and ydcW are separated by 21 nucleotides
b1441: ydcT - predicted spermidine/putrescine transporter subunit (NCBI), at 1,510,841 to 1,511,854
ydcT
b1442: ydcU - predicted spermidine/putrescine transporter subunit (NCBI), at 1,511,855 to 1,512,796
ydcU
b1443: ydcV - predicted spermidine/putrescine transporter subunit (NCBI), at 1,512,786 to 1,513,580
ydcV
b1444: ydcW - medium chain aldehyde dehydrogenase (NCBI), at 1,513,602 to 1,515,026
ydcW
Position (kb)
1511
1512
1513 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1510.962 kb on - strand, within ydcT at 1511.021 kb on + strand, within ydcT at 1511.073 kb on + strand, within ydcT at 1511.302 kb on + strand, within ydcT at 1511.365 kb on - strand, within ydcT at 1511.428 kb on - strand, within ydcT at 1511.757 kb on - strand at 1511.782 kb on + strand at 1511.886 kb on + strand at 1511.886 kb on + strand at 1512.028 kb on - strand, within ydcU at 1512.111 kb on - strand, within ydcU at 1512.111 kb on - strand, within ydcU at 1512.133 kb on + strand, within ydcU at 1512.192 kb on - strand, within ydcU at 1512.192 kb on - strand, within ydcU at 1512.194 kb on + strand, within ydcU at 1512.269 kb on - strand, within ydcU at 1512.470 kb on - strand, within ydcU at 1512.754 kb on - strand at 1512.754 kb on - strand at 1512.889 kb on - strand, within ydcV at 1512.964 kb on - strand, within ydcV at 1512.964 kb on - strand, within ydcV at 1513.150 kb on - strand, within ydcV at 1513.161 kb on - strand, within ydcV at 1513.161 kb on - strand, within ydcV at 1513.229 kb on + strand, within ydcV at 1513.229 kb on + strand, within ydcV
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 1,510,962 - ydcT b1441 0.12 -0.7 1,511,021 + ydcT b1441 0.18 +0.5 1,511,073 + ydcT b1441 0.23 -1.2 1,511,302 + ydcT b1441 0.45 +1.2 1,511,365 - ydcT b1441 0.52 -0.2 1,511,428 - ydcT b1441 0.58 +1.7 1,511,757 - +0.1 1,511,782 + -3.2 1,511,886 + +0.8 1,511,886 + -1.7 1,512,028 - ydcU b1442 0.18 +2.1 1,512,111 - ydcU b1442 0.27 -1.1 1,512,111 - ydcU b1442 0.27 +1.4 1,512,133 + ydcU b1442 0.30 +0.9 1,512,192 - ydcU b1442 0.36 +1.1 1,512,192 - ydcU b1442 0.36 -1.7 1,512,194 + ydcU b1442 0.36 +2.0 1,512,269 - ydcU b1442 0.44 +2.0 1,512,470 - ydcU b1442 0.65 +1.7 1,512,754 - +1.1 1,512,754 - -0.5 1,512,889 - ydcV b1443 0.13 +2.9 1,512,964 - ydcV b1443 0.22 -2.2 1,512,964 - ydcV b1443 0.22 +2.1 1,513,150 - ydcV b1443 0.46 -1.3 1,513,161 - ydcV b1443 0.47 -0.3 1,513,161 - ydcV b1443 0.47 -0.2 1,513,229 + ydcV b1443 0.56 +1.1 1,513,229 + ydcV b1443 0.56 -0.5
Or see this region's nucleotide sequence