Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt paaK and paaX are separated by 100 nucleotides paaX and paaY overlap by 19 nucleotides paaY and ynbG are separated by 103 nucleotides ynbG and ydbA are separated by 161 nucleotides
b1398: paaK - phenylacetyl-CoA ligase (NCBI), at 1,460,149 to 1,461,462
paaK
b1399: paaX - DNA-binding transcriptional repressor of phenylacetic acid degradation, aryl-CoA responsive (NCBI), at 1,461,563 to 1,462,513
paaX
b1400: paaY - predicted hexapeptide repeat acetyltransferase (NCBI), at 1,462,495 to 1,463,085
paaY
b4674: ynbG - expressed protein (RefSeq), at 1,463,189 to 1,463,254
ynbG
b4492: ydbA - no description, at 1,463,416 to 1,465,928
ydbA
Position (kb)
1461
1462
1463 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1461.222 kb on - strand, within paaK at 1461.441 kb on + strand at 1461.562 kb on + strand at 1461.562 kb on + strand at 1461.638 kb on + strand at 1461.687 kb on + strand, within paaX at 1461.873 kb on + strand, within paaX at 1461.873 kb on + strand, within paaX at 1461.938 kb on + strand, within paaX at 1461.978 kb on + strand, within paaX at 1462.399 kb on - strand, within paaX at 1462.521 kb on + strand at 1462.521 kb on + strand at 1462.975 kb on + strand, within paaY at 1462.975 kb on + strand, within paaY at 1462.988 kb on + strand, within paaY at 1463.000 kb on + strand, within paaY at 1463.053 kb on + strand at 1463.053 kb on + strand at 1463.080 kb on + strand at 1463.082 kb on + strand at 1463.499 kb on - strand at 1463.499 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 1,461,222 - paaK b1398 0.82 +1.1 1,461,441 + +0.1 1,461,562 + +1.6 1,461,562 + +0.7 1,461,638 + -1.0 1,461,687 + paaX b1399 0.13 +0.3 1,461,873 + paaX b1399 0.33 -1.7 1,461,873 + paaX b1399 0.33 +1.1 1,461,938 + paaX b1399 0.39 -1.2 1,461,978 + paaX b1399 0.44 +0.1 1,462,399 - paaX b1399 0.88 -3.6 1,462,521 + +0.1 1,462,521 + +0.7 1,462,975 + paaY b1400 0.81 +0.1 1,462,975 + paaY b1400 0.81 -0.4 1,462,988 + paaY b1400 0.83 +0.1 1,463,000 + paaY b1400 0.85 -0.9 1,463,053 + +0.3 1,463,053 + +0.1 1,463,080 + -0.1 1,463,082 + +3.0 1,463,499 - -0.9 1,463,499 - +0.4
Or see this region's nucleotide sequence