Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydbH and ynbE overlap by 4 nucleotides ynbE and ydbL are separated by 7 nucleotides ydbL and feaR are separated by 171 nucleotides feaR and feaB are separated by 235 nucleotides
b1381: ydbH - hypothetical protein (NCBI), at 1,441,075 to 1,443,714
ydbH
b1382: ynbE - predicted lipoprotein (NCBI), at 1,443,711 to 1,443,896
ynbE
b1383: ydbL - orf, hypothetical protein (VIMSS), at 1,443,904 to 1,444,230
ydbL
b1384: feaR - DNA-binding transcriptional dual regulator (NCBI), at 1,444,402 to 1,445,307
feaR
b1385: feaB - phenylacetaldehyde dehydrogenase (VIMSS), at 1,445,543 to 1,447,042
feaB
Position (kb)
1444
1445
1446 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1443.414 kb on + strand, within ydbH at 1443.414 kb on + strand, within ydbH at 1443.455 kb on + strand at 1443.522 kb on - strand at 1443.524 kb on + strand at 1443.525 kb on + strand at 1443.525 kb on + strand at 1443.532 kb on - strand at 1443.541 kb on - strand at 1443.606 kb on + strand at 1443.614 kb on + strand at 1443.622 kb on - strand at 1443.644 kb on + strand at 1443.652 kb on - strand at 1443.662 kb on - strand at 1443.662 kb on - strand at 1443.683 kb on - strand at 1443.683 kb on - strand at 1443.783 kb on + strand, within ynbE at 1443.890 kb on + strand at 1443.908 kb on + strand at 1443.951 kb on + strand, within ydbL at 1443.956 kb on + strand, within ydbL at 1443.956 kb on + strand, within ydbL at 1443.963 kb on + strand, within ydbL at 1444.102 kb on + strand, within ydbL at 1444.250 kb on + strand at 1444.250 kb on + strand at 1444.256 kb on + strand at 1444.390 kb on - strand at 1444.537 kb on - strand, within feaR at 1444.581 kb on - strand, within feaR at 1444.611 kb on - strand, within feaR at 1444.640 kb on - strand, within feaR at 1444.787 kb on - strand, within feaR at 1444.845 kb on - strand, within feaR at 1444.866 kb on - strand, within feaR at 1444.866 kb on - strand, within feaR at 1444.872 kb on - strand, within feaR at 1444.935 kb on - strand, within feaR at 1445.072 kb on + strand, within feaR at 1445.230 kb on - strand at 1445.600 kb on - strand at 1445.620 kb on - strand at 1445.830 kb on + strand, within feaB at 1445.843 kb on - strand, within feaB at 1445.934 kb on - strand, within feaB at 1445.934 kb on - strand, within feaB at 1445.934 kb on - strand, within feaB at 1445.979 kb on - strand, within feaB at 1446.256 kb on + strand, within feaB at 1446.256 kb on - strand, within feaB at 1446.256 kb on - strand, within feaB
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 1,443,414 + ydbH b1381 0.89 -0.4 1,443,414 + ydbH b1381 0.89 +0.3 1,443,455 + -0.2 1,443,522 - -0.4 1,443,524 + +2.9 1,443,525 + -1.4 1,443,525 + +0.5 1,443,532 - +0.3 1,443,541 - -1.3 1,443,606 + +1.5 1,443,614 + +0.1 1,443,622 - +2.0 1,443,644 + -0.3 1,443,652 - -1.0 1,443,662 - -0.4 1,443,662 - -2.2 1,443,683 - +1.1 1,443,683 - +0.1 1,443,783 + ynbE b1382 0.39 -1.4 1,443,890 + +0.4 1,443,908 + +2.4 1,443,951 + ydbL b1383 0.14 +0.1 1,443,956 + ydbL b1383 0.16 +0.4 1,443,956 + ydbL b1383 0.16 +2.1 1,443,963 + ydbL b1383 0.18 +0.4 1,444,102 + ydbL b1383 0.61 -0.6 1,444,250 + -2.0 1,444,250 + -0.6 1,444,256 + +0.7 1,444,390 - -1.4 1,444,537 - feaR b1384 0.15 -1.9 1,444,581 - feaR b1384 0.20 +2.5 1,444,611 - feaR b1384 0.23 +0.6 1,444,640 - feaR b1384 0.26 +1.0 1,444,787 - feaR b1384 0.42 +2.0 1,444,845 - feaR b1384 0.49 +1.8 1,444,866 - feaR b1384 0.51 +1.1 1,444,866 - feaR b1384 0.51 +1.1 1,444,872 - feaR b1384 0.52 +1.5 1,444,935 - feaR b1384 0.59 -0.9 1,445,072 + feaR b1384 0.74 +0.1 1,445,230 - -2.2 1,445,600 - -0.2 1,445,620 - -0.7 1,445,830 + feaB b1385 0.19 -0.8 1,445,843 - feaB b1385 0.20 +0.3 1,445,934 - feaB b1385 0.26 -0.0 1,445,934 - feaB b1385 0.26 +2.1 1,445,934 - feaB b1385 0.26 +0.6 1,445,979 - feaB b1385 0.29 +0.1 1,446,256 + feaB b1385 0.48 +3.1 1,446,256 - feaB b1385 0.48 +2.2 1,446,256 - feaB b1385 0.48 +0.1
Or see this region's nucleotide sequence