Strain Fitness in Escherichia coli BW25113 around b4527

Experiment: Cisplatin 0.1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydaD and sieB are separated by 441 nucleotidessieB and b1354 overlap by 4 nucleotidesb1354 and b1355 are separated by 10 nucleotidesb1355 and racR are separated by 308 nucleotides b1352: ydaD - orf, hypothetical protein (VIMSS), at 1,416,032 to 1,416,253 ydaD b1353: sieB - phage superinfection exclusion protein (VIMSS), at 1,416,695 to 1,417,183 sieB b4527: b1354 - orf, hypothetical protein (VIMSS), at 1,417,180 to 1,417,335 b1354 b1355: b1355 - orf, hypothetical protein (VIMSS), at 1,417,346 to 1,417,480 b1355 b1356: racR - Rac prophage; predicted DNA-binding transcriptional regulator (NCBI), at 1,417,789 to 1,418,265 racR Position (kb) 1417 1418Strain fitness (log2 ratio) -2 -1 0 1 2at 1416.473 kb on + strandat 1416.624 kb on - strandat 1416.644 kb on - strandat 1416.679 kb on - strandat 1416.958 kb on - strand, within sieBat 1417.076 kb on - strand, within sieBat 1417.079 kb on - strand, within sieBat 1417.082 kb on - strand, within sieBat 1417.082 kb on - strand, within sieBat 1417.082 kb on - strand, within sieBat 1417.082 kb on - strand, within sieBat 1417.082 kb on - strand, within sieBat 1417.082 kb on - strand, within sieBat 1417.092 kb on - strand, within sieBat 1417.101 kb on - strand, within sieBat 1417.190 kb on - strandat 1417.200 kb on - strand, within b1354at 1417.207 kb on - strand, within b1354at 1417.207 kb on - strand, within b1354at 1417.276 kb on - strand, within b1354at 1417.276 kb on - strand, within b1354at 1417.312 kb on - strand, within b1354at 1417.371 kb on - strand, within b1355at 1417.427 kb on + strand, within b1355at 1417.431 kb on - strand, within b1355at 1417.459 kb on + strand, within b1355at 1417.518 kb on - strandat 1417.574 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml
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1,416,473 + -2.3
1,416,624 - -1.2
1,416,644 - -1.2
1,416,679 - -0.2
1,416,958 - sieB b1353 0.54 -0.3
1,417,076 - sieB b1353 0.78 -1.9
1,417,079 - sieB b1353 0.79 +1.1
1,417,082 - sieB b1353 0.79 -1.2
1,417,082 - sieB b1353 0.79 +1.4
1,417,082 - sieB b1353 0.79 -1.0
1,417,082 - sieB b1353 0.79 -0.9
1,417,082 - sieB b1353 0.79 -1.2
1,417,082 - sieB b1353 0.79 -1.9
1,417,092 - sieB b1353 0.81 +1.3
1,417,101 - sieB b1353 0.83 -0.0
1,417,190 - -1.2
1,417,200 - b1354 b4527 0.13 -0.7
1,417,207 - b1354 b4527 0.17 -0.4
1,417,207 - b1354 b4527 0.17 -0.9
1,417,276 - b1354 b4527 0.62 +0.1
1,417,276 - b1354 b4527 0.62 -0.4
1,417,312 - b1354 b4527 0.85 -0.4
1,417,371 - b1355 b1355 0.19 -1.0
1,417,427 + b1355 b1355 0.60 +2.0
1,417,431 - b1355 b1355 0.63 -2.1
1,417,459 + b1355 b1355 0.84 -0.9
1,417,518 - -1.2
1,417,574 + +2.4

Or see this region's nucleotide sequence