Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydaD and sieB are separated by 441 nucleotides sieB and b1354 overlap by 4 nucleotides b1354 and b1355 are separated by 10 nucleotides b1355 and racR are separated by 308 nucleotides
b1352: ydaD - orf, hypothetical protein (VIMSS), at 1,416,032 to 1,416,253
ydaD
b1353: sieB - phage superinfection exclusion protein (VIMSS), at 1,416,695 to 1,417,183
sieB
b4527: b1354 - orf, hypothetical protein (VIMSS), at 1,417,180 to 1,417,335
b1354
b1355: b1355 - orf, hypothetical protein (VIMSS), at 1,417,346 to 1,417,480
b1355
b1356: racR - Rac prophage; predicted DNA-binding transcriptional regulator (NCBI), at 1,417,789 to 1,418,265
racR
Position (kb)
1417
1418 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1416.473 kb on + strand at 1416.624 kb on - strand at 1416.644 kb on - strand at 1416.679 kb on - strand at 1416.958 kb on - strand, within sieB at 1417.076 kb on - strand, within sieB at 1417.079 kb on - strand, within sieB at 1417.082 kb on - strand, within sieB at 1417.082 kb on - strand, within sieB at 1417.082 kb on - strand, within sieB at 1417.082 kb on - strand, within sieB at 1417.082 kb on - strand, within sieB at 1417.082 kb on - strand, within sieB at 1417.092 kb on - strand, within sieB at 1417.101 kb on - strand, within sieB at 1417.190 kb on - strand at 1417.200 kb on - strand, within b1354 at 1417.207 kb on - strand, within b1354 at 1417.207 kb on - strand, within b1354 at 1417.276 kb on - strand, within b1354 at 1417.276 kb on - strand, within b1354 at 1417.312 kb on - strand, within b1354 at 1417.371 kb on - strand, within b1355 at 1417.427 kb on + strand, within b1355 at 1417.431 kb on - strand, within b1355 at 1417.459 kb on + strand, within b1355 at 1417.518 kb on - strand at 1417.574 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 1,416,473 + -2.3 1,416,624 - -1.2 1,416,644 - -1.2 1,416,679 - -0.2 1,416,958 - sieB b1353 0.54 -0.3 1,417,076 - sieB b1353 0.78 -1.9 1,417,079 - sieB b1353 0.79 +1.1 1,417,082 - sieB b1353 0.79 -1.2 1,417,082 - sieB b1353 0.79 +1.4 1,417,082 - sieB b1353 0.79 -1.0 1,417,082 - sieB b1353 0.79 -0.9 1,417,082 - sieB b1353 0.79 -1.2 1,417,082 - sieB b1353 0.79 -1.9 1,417,092 - sieB b1353 0.81 +1.3 1,417,101 - sieB b1353 0.83 -0.0 1,417,190 - -1.2 1,417,200 - b1354 b4527 0.13 -0.7 1,417,207 - b1354 b4527 0.17 -0.4 1,417,207 - b1354 b4527 0.17 -0.9 1,417,276 - b1354 b4527 0.62 +0.1 1,417,276 - b1354 b4527 0.62 -0.4 1,417,312 - b1354 b4527 0.85 -0.4 1,417,371 - b1355 b1355 0.19 -1.0 1,417,427 + b1355 b1355 0.60 +2.0 1,417,431 - b1355 b1355 0.63 -2.1 1,417,459 + b1355 b1355 0.84 -0.9 1,417,518 - -1.2 1,417,574 + +2.4
Or see this region's nucleotide sequence