Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt btuR and yciK overlap by 4 nucleotides yciK and sohB are separated by 219 nucleotides sohB and yciN are separated by 35 nucleotides yciN and topA are separated by 379 nucleotides
b1270: btuR - cob(I)yrinic acid a,c-diamide adenosyltransferase (NCBI), at 1,325,791 to 1,326,381
btuR
b1271: yciK - short chain dehydrogenase (NCBI), at 1,326,378 to 1,327,136
yciK
b1272: sohB - predicted inner membrane peptidase (NCBI), at 1,327,356 to 1,328,405
sohB
b1273: yciN - hypothetical protein (NCBI), at 1,328,441 to 1,328,692
yciN
b1274: topA - DNA topoisomerase I (NCBI), at 1,329,072 to 1,331,669
topA
Position (kb)
1327
1328
1329 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1326.420 kb on + strand at 1326.420 kb on + strand at 1326.546 kb on + strand, within yciK at 1326.719 kb on + strand, within yciK at 1326.742 kb on - strand, within yciK at 1326.851 kb on - strand, within yciK at 1327.275 kb on + strand at 1327.297 kb on + strand at 1327.500 kb on + strand, within sohB at 1327.505 kb on + strand, within sohB at 1327.505 kb on + strand, within sohB at 1327.511 kb on - strand, within sohB at 1327.511 kb on - strand, within sohB at 1327.565 kb on + strand, within sohB at 1327.565 kb on + strand, within sohB at 1327.752 kb on - strand, within sohB at 1327.752 kb on - strand, within sohB at 1327.797 kb on + strand, within sohB at 1327.797 kb on + strand, within sohB at 1327.902 kb on - strand, within sohB at 1328.003 kb on + strand, within sohB at 1328.032 kb on - strand, within sohB at 1328.039 kb on + strand, within sohB at 1328.039 kb on + strand, within sohB at 1328.144 kb on + strand, within sohB at 1328.162 kb on + strand, within sohB at 1328.202 kb on - strand, within sohB at 1328.211 kb on + strand, within sohB at 1328.211 kb on + strand, within sohB at 1328.364 kb on + strand at 1328.556 kb on + strand, within yciN at 1328.569 kb on + strand, within yciN at 1328.713 kb on + strand at 1328.713 kb on + strand at 1328.829 kb on + strand at 1328.918 kb on - strand at 1328.962 kb on - strand at 1328.965 kb on + strand at 1328.965 kb on + strand at 1329.065 kb on + strand at 1329.065 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 1,326,420 + +1.0 1,326,420 + +0.2 1,326,546 + yciK b1271 0.22 -0.2 1,326,719 + yciK b1271 0.45 -0.8 1,326,742 - yciK b1271 0.48 +0.2 1,326,851 - yciK b1271 0.62 +0.2 1,327,275 + -0.8 1,327,297 + -0.9 1,327,500 + sohB b1272 0.14 -1.6 1,327,505 + sohB b1272 0.14 -1.0 1,327,505 + sohB b1272 0.14 -0.6 1,327,511 - sohB b1272 0.15 +2.7 1,327,511 - sohB b1272 0.15 +0.2 1,327,565 + sohB b1272 0.20 -2.5 1,327,565 + sohB b1272 0.20 +1.2 1,327,752 - sohB b1272 0.38 -0.3 1,327,752 - sohB b1272 0.38 -0.1 1,327,797 + sohB b1272 0.42 -0.8 1,327,797 + sohB b1272 0.42 +0.2 1,327,902 - sohB b1272 0.52 +0.2 1,328,003 + sohB b1272 0.62 -0.5 1,328,032 - sohB b1272 0.64 -3.2 1,328,039 + sohB b1272 0.65 -0.8 1,328,039 + sohB b1272 0.65 -0.8 1,328,144 + sohB b1272 0.75 -1.5 1,328,162 + sohB b1272 0.77 -0.3 1,328,202 - sohB b1272 0.81 -2.2 1,328,211 + sohB b1272 0.81 -1.2 1,328,211 + sohB b1272 0.81 +0.9 1,328,364 + -0.1 1,328,556 + yciN b1273 0.46 -1.8 1,328,569 + yciN b1273 0.51 -1.7 1,328,713 + +4.4 1,328,713 + -0.6 1,328,829 + -0.3 1,328,918 - -2.7 1,328,962 - -0.8 1,328,965 + +2.6 1,328,965 + -1.3 1,329,065 + +0.2 1,329,065 + -0.3
Or see this region's nucleotide sequence