Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt holB and ycfH are separated by 10 nucleotides ycfH and ptsG are separated by 294 nucleotides
b1099: holB - DNA polymerase III subunit delta' (NCBI), at 1,154,985 to 1,155,989
holB
b1100: ycfH - predicted metallodependent hydrolase (NCBI), at 1,156,000 to 1,156,797
ycfH
b1101: ptsG - fused glucose-specific PTS enzymes: IIB component/IIC component (NCBI), at 1,157,092 to 1,158,525
ptsG
Position (kb)
1155
1156
1157 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1155.056 kb on + strand at 1155.960 kb on + strand at 1155.960 kb on + strand at 1155.966 kb on + strand at 1156.034 kb on - strand at 1156.056 kb on + strand at 1156.056 kb on + strand at 1156.065 kb on + strand at 1156.078 kb on - strand at 1156.157 kb on + strand, within ycfH at 1156.157 kb on + strand, within ycfH at 1156.258 kb on - strand, within ycfH at 1156.318 kb on - strand, within ycfH at 1156.318 kb on - strand, within ycfH at 1156.358 kb on + strand, within ycfH at 1156.358 kb on + strand, within ycfH at 1156.392 kb on - strand, within ycfH at 1156.392 kb on - strand, within ycfH at 1156.392 kb on - strand, within ycfH at 1156.392 kb on - strand, within ycfH at 1156.456 kb on + strand, within ycfH at 1156.484 kb on - strand, within ycfH at 1156.513 kb on - strand, within ycfH at 1156.513 kb on - strand, within ycfH at 1156.568 kb on + strand, within ycfH at 1156.568 kb on + strand, within ycfH at 1156.598 kb on - strand, within ycfH at 1156.598 kb on - strand, within ycfH at 1156.602 kb on + strand, within ycfH at 1156.602 kb on + strand, within ycfH at 1156.602 kb on + strand, within ycfH at 1156.617 kb on + strand, within ycfH at 1156.622 kb on + strand, within ycfH at 1156.636 kb on - strand, within ycfH at 1156.636 kb on - strand, within ycfH at 1156.686 kb on + strand, within ycfH at 1156.692 kb on - strand, within ycfH at 1156.696 kb on + strand, within ycfH at 1156.698 kb on + strand, within ycfH at 1156.698 kb on + strand, within ycfH at 1156.698 kb on + strand, within ycfH at 1156.731 kb on + strand at 1157.020 kb on + strand at 1157.020 kb on + strand at 1157.020 kb on - strand at 1157.027 kb on + strand at 1157.072 kb on + strand at 1157.073 kb on + strand at 1157.080 kb on - strand at 1157.080 kb on - strand at 1157.152 kb on + strand at 1157.216 kb on + strand at 1157.216 kb on + strand at 1157.237 kb on - strand, within ptsG at 1157.288 kb on + strand, within ptsG at 1157.313 kb on - strand, within ptsG at 1157.313 kb on - strand, within ptsG at 1157.440 kb on + strand, within ptsG at 1157.440 kb on + strand, within ptsG at 1157.454 kb on + strand, within ptsG at 1157.454 kb on + strand, within ptsG at 1157.509 kb on - strand, within ptsG at 1157.509 kb on - strand, within ptsG at 1157.558 kb on + strand, within ptsG at 1157.625 kb on + strand, within ptsG at 1157.629 kb on - strand, within ptsG at 1157.629 kb on - strand, within ptsG at 1157.644 kb on - strand, within ptsG at 1157.650 kb on + strand, within ptsG at 1157.651 kb on - strand, within ptsG at 1157.671 kb on - strand, within ptsG at 1157.727 kb on - strand, within ptsG at 1157.761 kb on + strand, within ptsG
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 1,155,056 + -1.9 1,155,960 + -1.2 1,155,960 + +1.1 1,155,966 + -0.3 1,156,034 - -1.0 1,156,056 + -0.8 1,156,056 + +0.2 1,156,065 + -0.2 1,156,078 - +1.3 1,156,157 + ycfH b1100 0.20 +1.3 1,156,157 + ycfH b1100 0.20 +0.7 1,156,258 - ycfH b1100 0.32 -1.8 1,156,318 - ycfH b1100 0.40 +0.7 1,156,318 - ycfH b1100 0.40 +1.0 1,156,358 + ycfH b1100 0.45 -1.7 1,156,358 + ycfH b1100 0.45 +0.8 1,156,392 - ycfH b1100 0.49 -1.0 1,156,392 - ycfH b1100 0.49 +1.9 1,156,392 - ycfH b1100 0.49 -0.1 1,156,392 - ycfH b1100 0.49 -1.3 1,156,456 + ycfH b1100 0.57 -2.5 1,156,484 - ycfH b1100 0.61 -1.0 1,156,513 - ycfH b1100 0.64 +0.9 1,156,513 - ycfH b1100 0.64 -0.7 1,156,568 + ycfH b1100 0.71 -1.5 1,156,568 + ycfH b1100 0.71 -0.7 1,156,598 - ycfH b1100 0.75 -0.7 1,156,598 - ycfH b1100 0.75 +2.0 1,156,602 + ycfH b1100 0.75 +2.7 1,156,602 + ycfH b1100 0.75 +1.3 1,156,602 + ycfH b1100 0.75 +2.6 1,156,617 + ycfH b1100 0.77 +1.8 1,156,622 + ycfH b1100 0.78 +2.3 1,156,636 - ycfH b1100 0.80 -0.5 1,156,636 - ycfH b1100 0.80 +2.9 1,156,686 + ycfH b1100 0.86 +1.1 1,156,692 - ycfH b1100 0.87 +2.0 1,156,696 + ycfH b1100 0.87 +0.5 1,156,698 + ycfH b1100 0.87 +1.3 1,156,698 + ycfH b1100 0.87 +1.5 1,156,698 + ycfH b1100 0.87 -1.3 1,156,731 + -0.5 1,157,020 + -2.0 1,157,020 + -1.3 1,157,020 - -0.8 1,157,027 + +0.3 1,157,072 + +0.5 1,157,073 + -0.7 1,157,080 - -0.7 1,157,080 - +0.1 1,157,152 + -0.8 1,157,216 + -0.3 1,157,216 + -1.0 1,157,237 - ptsG b1101 0.10 +0.1 1,157,288 + ptsG b1101 0.14 +2.5 1,157,313 - ptsG b1101 0.15 +0.5 1,157,313 - ptsG b1101 0.15 -0.0 1,157,440 + ptsG b1101 0.24 +0.4 1,157,440 + ptsG b1101 0.24 +1.5 1,157,454 + ptsG b1101 0.25 -0.0 1,157,454 + ptsG b1101 0.25 -1.0 1,157,509 - ptsG b1101 0.29 -0.3 1,157,509 - ptsG b1101 0.29 +2.7 1,157,558 + ptsG b1101 0.32 +2.7 1,157,625 + ptsG b1101 0.37 +0.8 1,157,629 - ptsG b1101 0.37 +1.3 1,157,629 - ptsG b1101 0.37 -0.7 1,157,644 - ptsG b1101 0.38 +1.4 1,157,650 + ptsG b1101 0.39 -0.2 1,157,651 - ptsG b1101 0.39 +0.3 1,157,671 - ptsG b1101 0.40 +1.3 1,157,727 - ptsG b1101 0.44 +1.6 1,157,761 + ptsG b1101 0.47 +0.3
Or see this region's nucleotide sequence