Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yceG and tmk overlap by 11 nucleotides tmk and holB overlap by 4 nucleotides holB and ycfH are separated by 10 nucleotides
b1097: yceG - predicted aminodeoxychorismate lyase (NCBI), at 1,153,335 to 1,154,357
yceG
b1098: tmk - thymidylate kinase (NCBI), at 1,154,347 to 1,154,988
tmk
b1099: holB - DNA polymerase III subunit delta' (NCBI), at 1,154,985 to 1,155,989
holB
b1100: ycfH - predicted metallodependent hydrolase (NCBI), at 1,156,000 to 1,156,797
ycfH
Position (kb)
1154
1155
1156 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1154.104 kb on - strand, within yceG at 1154.150 kb on + strand, within yceG at 1154.150 kb on + strand, within yceG at 1154.152 kb on - strand, within yceG at 1154.152 kb on - strand, within yceG at 1154.158 kb on - strand, within yceG at 1154.158 kb on - strand, within yceG at 1154.160 kb on - strand, within yceG at 1154.280 kb on - strand at 1154.323 kb on + strand at 1154.323 kb on + strand at 1155.056 kb on + strand at 1155.960 kb on + strand at 1155.960 kb on + strand at 1155.966 kb on + strand at 1156.034 kb on - strand at 1156.056 kb on + strand at 1156.056 kb on + strand at 1156.065 kb on + strand at 1156.078 kb on - strand at 1156.157 kb on + strand, within ycfH at 1156.157 kb on + strand, within ycfH at 1156.258 kb on - strand, within ycfH at 1156.318 kb on - strand, within ycfH at 1156.318 kb on - strand, within ycfH at 1156.358 kb on + strand, within ycfH at 1156.358 kb on + strand, within ycfH at 1156.392 kb on - strand, within ycfH at 1156.392 kb on - strand, within ycfH at 1156.392 kb on - strand, within ycfH at 1156.392 kb on - strand, within ycfH at 1156.456 kb on + strand, within ycfH at 1156.484 kb on - strand, within ycfH at 1156.513 kb on - strand, within ycfH at 1156.513 kb on - strand, within ycfH at 1156.568 kb on + strand, within ycfH at 1156.568 kb on + strand, within ycfH at 1156.598 kb on - strand, within ycfH at 1156.598 kb on - strand, within ycfH at 1156.602 kb on + strand, within ycfH at 1156.602 kb on + strand, within ycfH at 1156.602 kb on + strand, within ycfH at 1156.617 kb on + strand, within ycfH at 1156.622 kb on + strand, within ycfH at 1156.636 kb on - strand, within ycfH at 1156.636 kb on - strand, within ycfH at 1156.686 kb on + strand, within ycfH at 1156.692 kb on - strand, within ycfH at 1156.696 kb on + strand, within ycfH at 1156.698 kb on + strand, within ycfH at 1156.698 kb on + strand, within ycfH at 1156.698 kb on + strand, within ycfH at 1156.731 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 1,154,104 - yceG b1097 0.75 -2.4 1,154,150 + yceG b1097 0.80 -1.1 1,154,150 + yceG b1097 0.80 -0.2 1,154,152 - yceG b1097 0.80 -0.5 1,154,152 - yceG b1097 0.80 +0.3 1,154,158 - yceG b1097 0.80 +1.3 1,154,158 - yceG b1097 0.80 -0.3 1,154,160 - yceG b1097 0.81 -0.9 1,154,280 - -0.3 1,154,323 + -0.7 1,154,323 + +0.6 1,155,056 + -1.9 1,155,960 + -1.2 1,155,960 + +1.1 1,155,966 + -0.3 1,156,034 - -1.0 1,156,056 + -0.8 1,156,056 + +0.2 1,156,065 + -0.2 1,156,078 - +1.3 1,156,157 + ycfH b1100 0.20 +1.3 1,156,157 + ycfH b1100 0.20 +0.7 1,156,258 - ycfH b1100 0.32 -1.8 1,156,318 - ycfH b1100 0.40 +0.7 1,156,318 - ycfH b1100 0.40 +1.0 1,156,358 + ycfH b1100 0.45 -1.7 1,156,358 + ycfH b1100 0.45 +0.8 1,156,392 - ycfH b1100 0.49 -1.0 1,156,392 - ycfH b1100 0.49 +1.9 1,156,392 - ycfH b1100 0.49 -0.1 1,156,392 - ycfH b1100 0.49 -1.3 1,156,456 + ycfH b1100 0.57 -2.5 1,156,484 - ycfH b1100 0.61 -1.0 1,156,513 - ycfH b1100 0.64 +0.9 1,156,513 - ycfH b1100 0.64 -0.7 1,156,568 + ycfH b1100 0.71 -1.5 1,156,568 + ycfH b1100 0.71 -0.7 1,156,598 - ycfH b1100 0.75 -0.7 1,156,598 - ycfH b1100 0.75 +2.0 1,156,602 + ycfH b1100 0.75 +2.7 1,156,602 + ycfH b1100 0.75 +1.3 1,156,602 + ycfH b1100 0.75 +2.6 1,156,617 + ycfH b1100 0.77 +1.8 1,156,622 + ycfH b1100 0.78 +2.3 1,156,636 - ycfH b1100 0.80 -0.5 1,156,636 - ycfH b1100 0.80 +2.9 1,156,686 + ycfH b1100 0.86 +1.1 1,156,692 - ycfH b1100 0.87 +2.0 1,156,696 + ycfH b1100 0.87 +0.5 1,156,698 + ycfH b1100 0.87 +1.3 1,156,698 + ycfH b1100 0.87 +1.5 1,156,698 + ycfH b1100 0.87 -1.3 1,156,731 + -0.5
Or see this region's nucleotide sequence