Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt serX and ycdW are separated by 233 nucleotides ycdW and ycdX are separated by 54 nucleotides ycdX and ycdY are separated by 23 nucleotides
b1032: serX - tRNA-Ser (NCBI), at 1,096,788 to 1,096,875
serX
b1033: ycdW - putative dehydrogenase (VIMSS), at 1,097,109 to 1,098,047
ycdW
b1034: ycdX - hypothetical protein (NCBI), at 1,098,102 to 1,098,839
ycdX
b1035: ycdY - hypothetical protein (NCBI), at 1,098,863 to 1,099,417
ycdY
Position (kb)
1097
1098
1099 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1096.126 kb on + strand at 1096.133 kb on - strand at 1096.134 kb on + strand at 1096.134 kb on + strand at 1096.142 kb on - strand at 1096.158 kb on + strand at 1096.174 kb on + strand at 1096.381 kb on - strand at 1096.893 kb on - strand at 1096.893 kb on - strand at 1097.109 kb on + strand at 1097.115 kb on - strand at 1097.173 kb on - strand at 1097.180 kb on - strand at 1097.226 kb on + strand, within ycdW at 1097.339 kb on + strand, within ycdW at 1097.503 kb on - strand, within ycdW at 1097.503 kb on - strand, within ycdW at 1097.550 kb on - strand, within ycdW at 1097.712 kb on + strand, within ycdW at 1097.712 kb on + strand, within ycdW at 1097.736 kb on - strand, within ycdW at 1097.773 kb on + strand, within ycdW at 1097.773 kb on + strand, within ycdW at 1097.786 kb on - strand, within ycdW at 1097.786 kb on - strand, within ycdW at 1097.787 kb on - strand, within ycdW at 1097.787 kb on - strand, within ycdW at 1097.836 kb on - strand, within ycdW at 1097.934 kb on - strand, within ycdW at 1097.941 kb on + strand, within ycdW at 1098.011 kb on + strand at 1098.107 kb on - strand at 1098.107 kb on - strand at 1098.140 kb on + strand at 1098.174 kb on + strand at 1098.250 kb on + strand, within ycdX at 1098.274 kb on - strand, within ycdX at 1098.295 kb on + strand, within ycdX at 1098.303 kb on - strand, within ycdX at 1098.304 kb on - strand, within ycdX at 1098.304 kb on - strand, within ycdX at 1098.390 kb on - strand, within ycdX at 1098.390 kb on - strand, within ycdX at 1098.447 kb on - strand, within ycdX at 1098.459 kb on + strand, within ycdX at 1098.465 kb on - strand, within ycdX at 1098.465 kb on - strand, within ycdX at 1098.656 kb on - strand, within ycdX at 1098.800 kb on + strand at 1098.805 kb on + strand at 1098.805 kb on + strand at 1098.810 kb on + strand at 1098.813 kb on - strand at 1098.813 kb on - strand at 1098.826 kb on + strand at 1098.888 kb on - strand at 1098.892 kb on + strand at 1098.924 kb on + strand, within ycdY at 1098.998 kb on + strand, within ycdY at 1099.046 kb on + strand, within ycdY
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 1,096,126 + -0.1 1,096,133 - -1.9 1,096,134 + -1.3 1,096,134 + +1.6 1,096,142 - -3.1 1,096,158 + +2.1 1,096,174 + +2.6 1,096,381 - -0.8 1,096,893 - +1.1 1,096,893 - -1.1 1,097,109 + +2.6 1,097,115 - -1.7 1,097,173 - +2.3 1,097,180 - -1.3 1,097,226 + ycdW b1033 0.12 -0.9 1,097,339 + ycdW b1033 0.24 -1.7 1,097,503 - ycdW b1033 0.42 -0.1 1,097,503 - ycdW b1033 0.42 -0.3 1,097,550 - ycdW b1033 0.47 -2.1 1,097,712 + ycdW b1033 0.64 +0.4 1,097,712 + ycdW b1033 0.64 +0.8 1,097,736 - ycdW b1033 0.67 -0.9 1,097,773 + ycdW b1033 0.71 +1.7 1,097,773 + ycdW b1033 0.71 -0.7 1,097,786 - ycdW b1033 0.72 +1.0 1,097,786 - ycdW b1033 0.72 -1.1 1,097,787 - ycdW b1033 0.72 -1.3 1,097,787 - ycdW b1033 0.72 -1.0 1,097,836 - ycdW b1033 0.77 -1.2 1,097,934 - ycdW b1033 0.88 -0.1 1,097,941 + ycdW b1033 0.89 -0.7 1,098,011 + +1.1 1,098,107 - -0.1 1,098,107 - -0.3 1,098,140 + -0.6 1,098,174 + +0.3 1,098,250 + ycdX b1034 0.20 +0.9 1,098,274 - ycdX b1034 0.23 -0.6 1,098,295 + ycdX b1034 0.26 +3.0 1,098,303 - ycdX b1034 0.27 -1.3 1,098,304 - ycdX b1034 0.27 +3.1 1,098,304 - ycdX b1034 0.27 +0.7 1,098,390 - ycdX b1034 0.39 +0.2 1,098,390 - ycdX b1034 0.39 -1.3 1,098,447 - ycdX b1034 0.47 +1.3 1,098,459 + ycdX b1034 0.48 +1.1 1,098,465 - ycdX b1034 0.49 +2.3 1,098,465 - ycdX b1034 0.49 -1.1 1,098,656 - ycdX b1034 0.75 -0.1 1,098,800 + -0.3 1,098,805 + -2.1 1,098,805 + -0.7 1,098,810 + +1.5 1,098,813 - -2.1 1,098,813 - -0.3 1,098,826 + -1.1 1,098,888 - +0.3 1,098,892 + +1.8 1,098,924 + ycdY b1035 0.11 -0.5 1,098,998 + ycdY b1035 0.24 +1.0 1,099,046 + ycdY b1035 0.33 +0.3
Or see this region's nucleotide sequence