Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycdB and phoH are separated by 344 nucleotides phoH and ycdP are separated by 49 nucleotides ycdP and ycdQ are separated by 1 nucleotides
b1019: ycdB - hypothetical protein (NCBI), at 1,082,599 to 1,083,870
ycdB
b1020: phoH - conserved protein with nucleoside triphosphate hydrolase domain (NCBI), at 1,084,215 to 1,085,279
phoH
b1021: ycdP - predicted inner membrane protein (NCBI), at 1,085,329 to 1,085,742
ycdP
b1022: ycdQ - predicted glycosyl transferase (NCBI), at 1,085,744 to 1,087,069
ycdQ
Position (kb)
1084
1085
1086 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1083.620 kb on + strand, within ycdB at 1083.620 kb on + strand, within ycdB at 1083.621 kb on + strand, within ycdB at 1083.650 kb on + strand, within ycdB at 1083.720 kb on - strand, within ycdB at 1083.787 kb on - strand at 1083.904 kb on + strand at 1084.000 kb on - strand at 1084.124 kb on - strand at 1084.407 kb on - strand, within phoH at 1084.446 kb on + strand, within phoH at 1084.494 kb on + strand, within phoH at 1084.561 kb on + strand, within phoH at 1084.620 kb on - strand, within phoH at 1084.765 kb on + strand, within phoH at 1084.860 kb on - strand, within phoH at 1084.946 kb on + strand, within phoH at 1084.999 kb on + strand, within phoH at 1085.048 kb on + strand, within phoH at 1085.201 kb on + strand at 1085.249 kb on + strand at 1085.249 kb on + strand at 1085.268 kb on + strand at 1085.277 kb on - strand at 1085.291 kb on + strand at 1085.292 kb on - strand at 1085.394 kb on + strand, within ycdP at 1085.394 kb on + strand, within ycdP at 1085.394 kb on + strand, within ycdP at 1085.426 kb on - strand, within ycdP at 1085.438 kb on + strand, within ycdP at 1085.438 kb on + strand, within ycdP at 1085.459 kb on - strand, within ycdP at 1085.459 kb on - strand, within ycdP at 1085.461 kb on + strand, within ycdP at 1085.461 kb on + strand, within ycdP at 1085.473 kb on + strand, within ycdP at 1085.473 kb on + strand, within ycdP at 1085.476 kb on + strand, within ycdP at 1085.484 kb on - strand, within ycdP at 1085.507 kb on - strand, within ycdP at 1085.646 kb on - strand, within ycdP at 1085.679 kb on + strand, within ycdP at 1085.759 kb on + strand at 1085.765 kb on + strand at 1085.782 kb on - strand at 1085.784 kb on - strand at 1085.791 kb on + strand at 1085.820 kb on + strand at 1085.823 kb on - strand at 1085.899 kb on + strand, within ycdQ at 1085.981 kb on + strand, within ycdQ at 1086.064 kb on - strand, within ycdQ at 1086.069 kb on - strand, within ycdQ at 1086.069 kb on - strand, within ycdQ at 1086.069 kb on - strand, within ycdQ at 1086.069 kb on - strand, within ycdQ at 1086.073 kb on - strand, within ycdQ at 1086.171 kb on - strand, within ycdQ at 1086.171 kb on - strand, within ycdQ at 1086.181 kb on - strand, within ycdQ at 1086.181 kb on - strand, within ycdQ at 1086.181 kb on - strand, within ycdQ at 1086.181 kb on - strand, within ycdQ at 1086.181 kb on - strand, within ycdQ at 1086.188 kb on - strand, within ycdQ at 1086.204 kb on - strand, within ycdQ at 1086.204 kb on - strand, within ycdQ at 1086.204 kb on - strand, within ycdQ at 1086.204 kb on - strand, within ycdQ at 1086.239 kb on - strand, within ycdQ at 1086.239 kb on - strand, within ycdQ at 1086.247 kb on - strand, within ycdQ at 1086.247 kb on - strand, within ycdQ at 1086.249 kb on - strand, within ycdQ at 1086.249 kb on - strand, within ycdQ at 1086.249 kb on - strand, within ycdQ at 1086.249 kb on - strand, within ycdQ at 1086.251 kb on - strand, within ycdQ
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 1,083,620 + ycdB b1019 0.80 +1.9 1,083,620 + ycdB b1019 0.80 -1.5 1,083,621 + ycdB b1019 0.80 -2.1 1,083,650 + ycdB b1019 0.83 +1.3 1,083,720 - ycdB b1019 0.88 -1.8 1,083,787 - -0.6 1,083,904 + +1.0 1,084,000 - -0.3 1,084,124 - -0.3 1,084,407 - phoH b1020 0.18 +3.2 1,084,446 + phoH b1020 0.22 -1.1 1,084,494 + phoH b1020 0.26 -1.2 1,084,561 + phoH b1020 0.32 -2.5 1,084,620 - phoH b1020 0.38 -3.2 1,084,765 + phoH b1020 0.52 -1.1 1,084,860 - phoH b1020 0.61 +0.0 1,084,946 + phoH b1020 0.69 -0.3 1,084,999 + phoH b1020 0.74 +1.2 1,085,048 + phoH b1020 0.78 +2.2 1,085,201 + -1.2 1,085,249 + +1.6 1,085,249 + -1.1 1,085,268 + +0.7 1,085,277 - -0.8 1,085,291 + +1.4 1,085,292 - -0.8 1,085,394 + ycdP b1021 0.16 +1.2 1,085,394 + ycdP b1021 0.16 +1.8 1,085,394 + ycdP b1021 0.16 -0.8 1,085,426 - ycdP b1021 0.23 +0.4 1,085,438 + ycdP b1021 0.26 -0.8 1,085,438 + ycdP b1021 0.26 +3.3 1,085,459 - ycdP b1021 0.31 -0.6 1,085,459 - ycdP b1021 0.31 +0.2 1,085,461 + ycdP b1021 0.32 +0.9 1,085,461 + ycdP b1021 0.32 -1.3 1,085,473 + ycdP b1021 0.35 +0.2 1,085,473 + ycdP b1021 0.35 +0.8 1,085,476 + ycdP b1021 0.36 +1.2 1,085,484 - ycdP b1021 0.37 +1.0 1,085,507 - ycdP b1021 0.43 +0.8 1,085,646 - ycdP b1021 0.77 -0.3 1,085,679 + ycdP b1021 0.85 -0.6 1,085,759 + +1.5 1,085,765 + -0.1 1,085,782 - -0.3 1,085,784 - -1.2 1,085,791 + -0.1 1,085,820 + -0.7 1,085,823 - -1.1 1,085,899 + ycdQ b1022 0.12 +1.2 1,085,981 + ycdQ b1022 0.18 -1.3 1,086,064 - ycdQ b1022 0.24 +0.2 1,086,069 - ycdQ b1022 0.25 +0.6 1,086,069 - ycdQ b1022 0.25 -1.3 1,086,069 - ycdQ b1022 0.25 +0.1 1,086,069 - ycdQ b1022 0.25 -0.2 1,086,073 - ycdQ b1022 0.25 -2.3 1,086,171 - ycdQ b1022 0.32 -0.8 1,086,171 - ycdQ b1022 0.32 -0.1 1,086,181 - ycdQ b1022 0.33 +2.1 1,086,181 - ycdQ b1022 0.33 -0.6 1,086,181 - ycdQ b1022 0.33 -1.3 1,086,181 - ycdQ b1022 0.33 -1.8 1,086,181 - ycdQ b1022 0.33 +3.7 1,086,188 - ycdQ b1022 0.33 -0.8 1,086,204 - ycdQ b1022 0.35 -1.9 1,086,204 - ycdQ b1022 0.35 +1.2 1,086,204 - ycdQ b1022 0.35 +0.8 1,086,204 - ycdQ b1022 0.35 -0.6 1,086,239 - ycdQ b1022 0.37 -0.2 1,086,239 - ycdQ b1022 0.37 -0.6 1,086,247 - ycdQ b1022 0.38 -0.3 1,086,247 - ycdQ b1022 0.38 -1.9 1,086,249 - ycdQ b1022 0.38 -0.3 1,086,249 - ycdQ b1022 0.38 +1.6 1,086,249 - ycdQ b1022 0.38 +0.9 1,086,249 - ycdQ b1022 0.38 -1.8 1,086,251 - ycdQ b1022 0.38 -1.3
Or see this region's nucleotide sequence