Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycdO and ycdB are separated by 5 nucleotides ycdB and phoH are separated by 344 nucleotides
b1018: ycdO - hypothetical protein (NCBI), at 1,081,466 to 1,082,593
ycdO
b1019: ycdB - hypothetical protein (NCBI), at 1,082,599 to 1,083,870
ycdB
b1020: phoH - conserved protein with nucleoside triphosphate hydrolase domain (NCBI), at 1,084,215 to 1,085,279
phoH
Position (kb)
1082
1083
1084 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1081.663 kb on + strand, within ycdO at 1081.663 kb on + strand, within ycdO at 1081.663 kb on - strand, within ycdO at 1081.663 kb on - strand, within ycdO at 1081.678 kb on - strand, within ycdO at 1081.852 kb on + strand, within ycdO at 1081.852 kb on + strand, within ycdO at 1081.908 kb on + strand, within ycdO at 1081.944 kb on + strand, within ycdO at 1081.979 kb on + strand, within ycdO at 1082.039 kb on + strand, within ycdO at 1082.039 kb on + strand, within ycdO at 1082.156 kb on - strand, within ycdO at 1082.179 kb on + strand, within ycdO at 1082.192 kb on - strand, within ycdO at 1082.382 kb on + strand, within ycdO at 1082.392 kb on + strand, within ycdO at 1082.515 kb on + strand at 1082.515 kb on + strand at 1082.594 kb on + strand at 1082.783 kb on - strand, within ycdB at 1082.787 kb on + strand, within ycdB at 1082.912 kb on + strand, within ycdB at 1083.109 kb on + strand, within ycdB at 1083.141 kb on - strand, within ycdB at 1083.141 kb on - strand, within ycdB at 1083.197 kb on + strand, within ycdB at 1083.212 kb on + strand, within ycdB at 1083.620 kb on + strand, within ycdB at 1083.620 kb on + strand, within ycdB at 1083.621 kb on + strand, within ycdB at 1083.650 kb on + strand, within ycdB at 1083.720 kb on - strand, within ycdB at 1083.787 kb on - strand at 1083.904 kb on + strand at 1084.000 kb on - strand at 1084.124 kb on - strand at 1084.407 kb on - strand, within phoH at 1084.446 kb on + strand, within phoH at 1084.494 kb on + strand, within phoH at 1084.561 kb on + strand, within phoH at 1084.620 kb on - strand, within phoH at 1084.765 kb on + strand, within phoH at 1084.860 kb on - strand, within phoH
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 1,081,663 + ycdO b1018 0.17 -0.9 1,081,663 + ycdO b1018 0.17 +0.8 1,081,663 - ycdO b1018 0.17 -1.6 1,081,663 - ycdO b1018 0.17 +1.8 1,081,678 - ycdO b1018 0.19 -1.4 1,081,852 + ycdO b1018 0.34 +0.0 1,081,852 + ycdO b1018 0.34 -2.5 1,081,908 + ycdO b1018 0.39 +2.2 1,081,944 + ycdO b1018 0.42 -1.4 1,081,979 + ycdO b1018 0.45 -0.6 1,082,039 + ycdO b1018 0.51 -0.6 1,082,039 + ycdO b1018 0.51 -0.6 1,082,156 - ycdO b1018 0.61 +0.9 1,082,179 + ycdO b1018 0.63 +1.0 1,082,192 - ycdO b1018 0.64 -1.4 1,082,382 + ycdO b1018 0.81 -0.8 1,082,392 + ycdO b1018 0.82 -0.3 1,082,515 + +1.2 1,082,515 + +2.2 1,082,594 + +0.9 1,082,783 - ycdB b1019 0.14 -1.8 1,082,787 + ycdB b1019 0.15 -1.9 1,082,912 + ycdB b1019 0.25 -0.2 1,083,109 + ycdB b1019 0.40 +0.4 1,083,141 - ycdB b1019 0.43 -0.4 1,083,141 - ycdB b1019 0.43 +2.1 1,083,197 + ycdB b1019 0.47 +0.7 1,083,212 + ycdB b1019 0.48 +0.2 1,083,620 + ycdB b1019 0.80 +1.9 1,083,620 + ycdB b1019 0.80 -1.5 1,083,621 + ycdB b1019 0.80 -2.1 1,083,650 + ycdB b1019 0.83 +1.3 1,083,720 - ycdB b1019 0.88 -1.8 1,083,787 - -0.6 1,083,904 + +1.0 1,084,000 - -0.3 1,084,124 - -0.3 1,084,407 - phoH b1020 0.18 +3.2 1,084,446 + phoH b1020 0.22 -1.1 1,084,494 + phoH b1020 0.26 -1.2 1,084,561 + phoH b1020 0.32 -2.5 1,084,620 - phoH b1020 0.38 -3.2 1,084,765 + phoH b1020 0.52 -1.1 1,084,860 - phoH b1020 0.61 +0.0
Or see this region's nucleotide sequence