Strain Fitness in Escherichia coli BW25113 around b1009

Experiment: Cisplatin 0.1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntb1007 and ycdI are separated by 10 nucleotidesycdI and rarA are separated by 9 nucleotidesrarA and ycdK are separated by 7 nucleotidesycdK and b1011 are separated by 11 nucleotides b1007: b1007 - orf, hypothetical protein (VIMSS), at 1,069,083 to 1,069,577 b1007 b1008: ycdI - hypothetical protein (NCBI), at 1,069,588 to 1,070,178 ycdI b1009: rarA - predicted hydrolase (NCBI), at 1,070,188 to 1,070,988 rarA b1010: ycdK - hypothetical protein (NCBI), at 1,070,996 to 1,071,382 ycdK b1011: b1011 - putative synthetase (VIMSS), at 1,071,394 to 1,072,086 b1011 Position (kb) 1070 1071Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1069.242 kb on + strand, within b1007at 1069.600 kb on + strandat 1069.660 kb on + strand, within ycdIat 1069.662 kb on - strand, within ycdIat 1069.662 kb on - strand, within ycdIat 1069.797 kb on + strand, within ycdIat 1069.797 kb on + strand, within ycdIat 1069.870 kb on - strand, within ycdIat 1069.870 kb on - strand, within ycdIat 1069.872 kb on + strand, within ycdIat 1069.872 kb on + strandat 1070.145 kb on - strandat 1070.145 kb on - strandat 1070.152 kb on - strandat 1070.241 kb on - strandat 1070.241 kb on - strandat 1070.569 kb on - strand, within rarAat 1070.580 kb on - strand, within rarAat 1070.580 kb on - strand, within rarAat 1070.633 kb on - strand, within rarAat 1070.633 kb on - strand, within rarAat 1070.678 kb on - strand, within rarAat 1070.684 kb on + strand, within rarAat 1070.689 kb on + strand, within rarAat 1070.840 kb on + strand, within rarAat 1070.863 kb on - strand, within rarAat 1070.875 kb on - strand, within rarAat 1070.875 kb on - strand, within rarAat 1070.956 kb on + strandat 1070.956 kb on + strandat 1070.988 kb on + strandat 1071.230 kb on - strand, within ycdKat 1071.301 kb on - strand, within ycdKat 1071.301 kb on - strand, within ycdKat 1071.301 kb on - strand, within ycdKat 1071.319 kb on - strand, within ycdKat 1071.343 kb on - strand, within ycdKat 1071.343 kb on - strand, within ycdKat 1071.382 kb on + strandat 1071.696 kb on + strand, within b1011at 1071.712 kb on + strand, within b1011at 1071.737 kb on - strand, within b1011

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Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml
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1,069,242 + b1007 b1007 0.32 +1.9
1,069,600 + +0.0
1,069,660 + ycdI b1008 0.12 -2.2
1,069,662 - ycdI b1008 0.13 -0.7
1,069,662 - ycdI b1008 0.13 -1.6
1,069,797 + ycdI b1008 0.35 +1.6
1,069,797 + ycdI b1008 0.35 +2.3
1,069,870 - ycdI b1008 0.48 +0.6
1,069,870 - ycdI b1008 0.48 +0.8
1,069,872 + ycdI b1008 0.48 -0.7
1,069,872 + +0.3
1,070,145 - -0.2
1,070,145 - -1.1
1,070,152 - -0.3
1,070,241 - +1.2
1,070,241 - -0.3
1,070,569 - rarA b1009 0.48 +3.2
1,070,580 - rarA b1009 0.49 +0.3
1,070,580 - rarA b1009 0.49 +1.2
1,070,633 - rarA b1009 0.56 +1.8
1,070,633 - rarA b1009 0.56 -0.3
1,070,678 - rarA b1009 0.61 +0.7
1,070,684 + rarA b1009 0.62 +2.7
1,070,689 + rarA b1009 0.63 -0.5
1,070,840 + rarA b1009 0.81 -1.1
1,070,863 - rarA b1009 0.84 +1.2
1,070,875 - rarA b1009 0.86 -1.1
1,070,875 - rarA b1009 0.86 +1.1
1,070,956 + +0.9
1,070,956 + -0.1
1,070,988 + +0.6
1,071,230 - ycdK b1010 0.60 -0.9
1,071,301 - ycdK b1010 0.79 -1.6
1,071,301 - ycdK b1010 0.79 +2.7
1,071,301 - ycdK b1010 0.79 -0.8
1,071,319 - ycdK b1010 0.83 -1.7
1,071,343 - ycdK b1010 0.90 +1.6
1,071,343 - ycdK b1010 0.90 -0.3
1,071,382 + +0.0
1,071,696 + b1011 b1011 0.44 +1.2
1,071,712 + b1011 b1011 0.46 +3.0
1,071,737 - b1011 b1011 0.49 -0.2

Or see this region's nucleotide sequence