Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt torA and torD overlap by 4 nucleotides torD and cbpM are separated by 151 nucleotides cbpM and cbpA overlap by 1 nucleotides
b0997: torA - trimethylamine N-oxide (TMAO) reductase I, catalytic subunit (NCBI), at 1,058,479 to 1,061,025
torA
b0998: torD - chaperone protein TorD (NCBI), at 1,061,022 to 1,061,621
torD
b0999: cbpM - modulator of CbpA co-chaperone (NCBI), at 1,061,773 to 1,062,078
cbpM
b1000: cbpA - curved DNA-binding protein, DnaJ homologue that functions as a co-chaperone of DnaK (NCBI), at 1,062,078 to 1,062,998
cbpA
Position (kb)
1061
1062 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1060.234 kb on - strand, within torA at 1060.234 kb on - strand, within torA at 1060.302 kb on - strand, within torA at 1060.302 kb on - strand, within torA at 1060.512 kb on + strand, within torA at 1060.744 kb on - strand, within torA at 1060.754 kb on + strand, within torA at 1060.754 kb on + strand, within torA at 1060.803 kb on + strand at 1060.826 kb on - strand at 1060.826 kb on - strand at 1060.847 kb on - strand at 1060.940 kb on + strand at 1060.989 kb on + strand at 1061.073 kb on + strand at 1061.083 kb on - strand, within torD at 1061.747 kb on - strand at 1061.789 kb on - strand at 1061.879 kb on - strand, within cbpM at 1061.889 kb on - strand, within cbpM at 1061.978 kb on - strand, within cbpM at 1061.978 kb on - strand, within cbpM at 1062.190 kb on + strand, within cbpA at 1062.191 kb on - strand, within cbpA at 1062.191 kb on - strand, within cbpA at 1062.401 kb on - strand, within cbpA at 1062.545 kb on - strand, within cbpA at 1062.572 kb on + strand, within cbpA
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 1,060,234 - torA b0997 0.69 -1.6 1,060,234 - torA b0997 0.69 +3.4 1,060,302 - torA b0997 0.72 +0.3 1,060,302 - torA b0997 0.72 -3.7 1,060,512 + torA b0997 0.80 +1.4 1,060,744 - torA b0997 0.89 +0.6 1,060,754 + torA b0997 0.89 +1.2 1,060,754 + torA b0997 0.89 -1.4 1,060,803 + -0.1 1,060,826 - -0.6 1,060,826 - +0.1 1,060,847 - -0.4 1,060,940 + -1.2 1,060,989 + -0.8 1,061,073 + +0.1 1,061,083 - torD b0998 0.10 -1.0 1,061,747 - +0.7 1,061,789 - +2.5 1,061,879 - cbpM b0999 0.35 -0.1 1,061,889 - cbpM b0999 0.38 -1.4 1,061,978 - cbpM b0999 0.67 +1.3 1,061,978 - cbpM b0999 0.67 -0.9 1,062,190 + cbpA b1000 0.12 +0.1 1,062,191 - cbpA b1000 0.12 -1.1 1,062,191 - cbpA b1000 0.12 +0.9 1,062,401 - cbpA b1000 0.35 +0.8 1,062,545 - cbpA b1000 0.51 +1.7 1,062,572 + cbpA b1000 0.54 +1.7
Or see this region's nucleotide sequence