Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hyaE and hyaF overlap by 4 nucleotides hyaF and appC are separated by 133 nucleotides appC and appB are separated by 11 nucleotides
b0976: hyaE - protein involved in processing of HyaA and HyaB proteins (NCBI), at 1,035,577 to 1,035,975
hyaE
b0977: hyaF - protein involved in nickel incorporation into hydrogenase-1 proteins (NCBI), at 1,035,972 to 1,036,829
hyaF
b0978: appC - cytochrome bd-II oxidase, subunit I (NCBI), at 1,036,963 to 1,038,507
appC
b0979: appB - cytochrome bd-II oxidase, subunit II (NCBI), at 1,038,519 to 1,039,655
appB
Position (kb)
1036
1037
1038
1039 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1035.990 kb on + strand at 1036.062 kb on - strand, within hyaF at 1036.216 kb on + strand, within hyaF at 1036.216 kb on + strand, within hyaF at 1036.275 kb on + strand, within hyaF at 1036.294 kb on + strand, within hyaF at 1036.394 kb on - strand, within hyaF at 1036.463 kb on + strand at 1036.690 kb on - strand, within hyaF at 1036.690 kb on - strand, within hyaF at 1036.774 kb on - strand at 1036.791 kb on - strand at 1036.863 kb on - strand at 1036.863 kb on - strand at 1037.041 kb on - strand at 1037.175 kb on + strand, within appC at 1037.240 kb on - strand, within appC at 1037.240 kb on - strand, within appC at 1037.262 kb on + strand, within appC at 1037.266 kb on + strand, within appC at 1037.367 kb on - strand, within appC at 1037.378 kb on + strand, within appC at 1037.396 kb on - strand, within appC at 1037.766 kb on + strand, within appC at 1037.904 kb on - strand, within appC at 1037.904 kb on - strand, within appC at 1037.934 kb on - strand, within appC at 1037.988 kb on - strand, within appC at 1037.988 kb on - strand, within appC at 1038.009 kb on - strand, within appC at 1038.093 kb on - strand, within appC at 1038.110 kb on - strand, within appC at 1038.170 kb on - strand, within appC at 1038.224 kb on - strand, within appC at 1038.248 kb on - strand, within appC at 1038.293 kb on - strand, within appC at 1038.293 kb on - strand, within appC at 1038.327 kb on - strand, within appC at 1038.336 kb on + strand, within appC at 1038.377 kb on + strand at 1038.395 kb on - strand at 1038.805 kb on - strand, within appB at 1039.201 kb on + strand, within appB at 1039.201 kb on + strand, within appB at 1039.201 kb on + strand, within appB at 1039.209 kb on - strand, within appB at 1039.209 kb on - strand, within appB at 1039.263 kb on + strand, within appB at 1039.453 kb on - strand, within appB at 1039.453 kb on - strand, within appB
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 1,035,990 + -2.3 1,036,062 - hyaF b0977 0.10 -0.8 1,036,216 + hyaF b0977 0.28 +0.7 1,036,216 + hyaF b0977 0.28 -1.8 1,036,275 + hyaF b0977 0.35 +2.0 1,036,294 + hyaF b0977 0.38 +0.6 1,036,394 - hyaF b0977 0.49 +0.6 1,036,463 + +2.2 1,036,690 - hyaF b0977 0.84 -0.2 1,036,690 - hyaF b0977 0.84 -0.4 1,036,774 - +1.4 1,036,791 - +0.2 1,036,863 - +0.2 1,036,863 - -0.6 1,037,041 - -0.0 1,037,175 + appC b0978 0.14 -0.4 1,037,240 - appC b0978 0.18 -0.4 1,037,240 - appC b0978 0.18 -0.8 1,037,262 + appC b0978 0.19 -0.6 1,037,266 + appC b0978 0.20 +0.2 1,037,367 - appC b0978 0.26 -0.5 1,037,378 + appC b0978 0.27 +0.2 1,037,396 - appC b0978 0.28 -1.3 1,037,766 + appC b0978 0.52 -1.4 1,037,904 - appC b0978 0.61 +1.1 1,037,904 - appC b0978 0.61 +2.9 1,037,934 - appC b0978 0.63 -1.2 1,037,988 - appC b0978 0.66 -0.9 1,037,988 - appC b0978 0.66 +0.5 1,038,009 - appC b0978 0.68 -0.7 1,038,093 - appC b0978 0.73 +0.9 1,038,110 - appC b0978 0.74 -0.3 1,038,170 - appC b0978 0.78 +0.4 1,038,224 - appC b0978 0.82 +0.7 1,038,248 - appC b0978 0.83 +0.2 1,038,293 - appC b0978 0.86 -0.1 1,038,293 - appC b0978 0.86 +0.2 1,038,327 - appC b0978 0.88 -0.4 1,038,336 + appC b0978 0.89 -1.4 1,038,377 + -1.6 1,038,395 - +1.0 1,038,805 - appB b0979 0.25 +0.7 1,039,201 + appB b0979 0.60 +0.5 1,039,201 + appB b0979 0.60 -0.6 1,039,201 + appB b0979 0.60 +2.6 1,039,209 - appB b0979 0.61 +1.2 1,039,209 - appB b0979 0.61 +0.6 1,039,263 + appB b0979 0.65 +1.2 1,039,453 - appB b0979 0.82 +2.1 1,039,453 - appB b0979 0.82 -1.5
Or see this region's nucleotide sequence