Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt b0960 and yccF are separated by 18 nucleotides yccF and helD are separated by 122 nucleotides helD and mgsA are separated by 31 nucleotides mgsA and yccT are separated by 95 nucleotides
b0960: b0960 - orf, hypothetical protein (VIMSS), at 1,020,953 to 1,023,106
b0960
b0961: yccF - conserved inner membrane protein (NCBI), at 1,023,125 to 1,023,571
yccF
b0962: helD - DNA helicase IV (NCBI), at 1,023,694 to 1,025,748
helD
b0963: mgsA - methylglyoxal synthase (VIMSS), at 1,025,780 to 1,026,238
mgsA
b0964: yccT - hypothetical protein (NCBI), at 1,026,334 to 1,026,996
yccT
Position (kb)
1023
1024
1025
1026 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1022.703 kb on + strand, within b0960 at 1022.713 kb on - strand, within b0960 at 1022.810 kb on + strand, within b0960 at 1022.819 kb on + strand, within b0960 at 1022.852 kb on - strand, within b0960 at 1022.884 kb on - strand, within b0960 at 1022.884 kb on - strand, within b0960 at 1022.886 kb on + strand, within b0960 at 1023.008 kb on + strand at 1023.160 kb on + strand at 1023.160 kb on + strand at 1023.170 kb on - strand, within yccF at 1023.179 kb on - strand, within yccF at 1023.400 kb on + strand, within yccF at 1023.423 kb on + strand, within yccF at 1023.423 kb on + strand, within yccF at 1023.488 kb on + strand, within yccF at 1023.512 kb on + strand, within yccF at 1023.525 kb on - strand, within yccF at 1023.525 kb on - strand, within yccF at 1023.588 kb on - strand at 1023.689 kb on - strand at 1023.689 kb on - strand at 1023.698 kb on - strand at 1023.770 kb on + strand at 1023.770 kb on + strand at 1023.867 kb on - strand at 1023.867 kb on - strand at 1023.876 kb on + strand at 1023.937 kb on - strand, within helD at 1024.110 kb on + strand, within helD at 1024.181 kb on - strand, within helD at 1024.387 kb on - strand, within helD at 1024.483 kb on - strand, within helD at 1024.483 kb on - strand, within helD at 1024.499 kb on + strand, within helD at 1024.801 kb on - strand, within helD at 1024.801 kb on - strand, within helD at 1024.971 kb on + strand, within helD at 1024.988 kb on + strand, within helD at 1024.988 kb on + strand, within helD at 1025.201 kb on - strand, within helD at 1025.201 kb on - strand, within helD at 1025.213 kb on + strand, within helD at 1025.213 kb on + strand, within helD at 1025.399 kb on + strand, within helD at 1025.424 kb on + strand, within helD at 1025.424 kb on + strand, within helD at 1025.661 kb on + strand at 1025.662 kb on + strand at 1025.669 kb on - strand at 1025.772 kb on + strand at 1025.827 kb on - strand, within mgsA at 1025.893 kb on - strand, within mgsA at 1025.921 kb on + strand, within mgsA at 1025.943 kb on - strand, within mgsA at 1025.943 kb on - strand, within mgsA at 1026.042 kb on - strand, within mgsA at 1026.043 kb on - strand at 1026.054 kb on + strand, within mgsA at 1026.115 kb on + strand, within mgsA at 1026.116 kb on - strand, within mgsA at 1026.119 kb on + strand, within mgsA at 1026.123 kb on - strand, within mgsA at 1026.153 kb on + strand, within mgsA at 1026.153 kb on + strand, within mgsA at 1026.295 kb on + strand at 1026.403 kb on - strand, within yccT at 1026.403 kb on - strand, within yccT at 1026.627 kb on + strand, within yccT at 1026.627 kb on + strand at 1026.685 kb on + strand, within yccT at 1026.740 kb on + strand, within yccT at 1026.740 kb on + strand, within yccT
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 1,022,703 + b0960 b0960 0.81 -2.2 1,022,713 - b0960 b0960 0.82 +0.2 1,022,810 + b0960 b0960 0.86 +0.2 1,022,819 + b0960 b0960 0.87 -0.0 1,022,852 - b0960 b0960 0.88 -0.9 1,022,884 - b0960 b0960 0.90 -2.9 1,022,884 - b0960 b0960 0.90 -2.2 1,022,886 + b0960 b0960 0.90 -1.3 1,023,008 + +1.2 1,023,160 + +1.7 1,023,160 + +0.2 1,023,170 - yccF b0961 0.10 -2.2 1,023,179 - yccF b0961 0.12 +0.8 1,023,400 + yccF b0961 0.62 +1.5 1,023,423 + yccF b0961 0.67 -1.4 1,023,423 + yccF b0961 0.67 -2.2 1,023,488 + yccF b0961 0.81 +0.7 1,023,512 + yccF b0961 0.87 +0.5 1,023,525 - yccF b0961 0.89 -0.8 1,023,525 - yccF b0961 0.89 -2.0 1,023,588 - +1.5 1,023,689 - +2.2 1,023,689 - +0.2 1,023,698 - +1.8 1,023,770 + -1.6 1,023,770 + -2.0 1,023,867 - +0.9 1,023,867 - +1.0 1,023,876 + +1.7 1,023,937 - helD b0962 0.12 -1.1 1,024,110 + helD b0962 0.20 -0.2 1,024,181 - helD b0962 0.24 +2.9 1,024,387 - helD b0962 0.34 -1.1 1,024,483 - helD b0962 0.38 -1.1 1,024,483 - helD b0962 0.38 -1.8 1,024,499 + helD b0962 0.39 -1.1 1,024,801 - helD b0962 0.54 -1.6 1,024,801 - helD b0962 0.54 -2.2 1,024,971 + helD b0962 0.62 +0.2 1,024,988 + helD b0962 0.63 -0.8 1,024,988 + helD b0962 0.63 +0.4 1,025,201 - helD b0962 0.73 -1.1 1,025,201 - helD b0962 0.73 +0.8 1,025,213 + helD b0962 0.74 +0.2 1,025,213 + helD b0962 0.74 -1.0 1,025,399 + helD b0962 0.83 -0.2 1,025,424 + helD b0962 0.84 +0.2 1,025,424 + helD b0962 0.84 +3.2 1,025,661 + -0.4 1,025,662 + +0.2 1,025,669 - +0.4 1,025,772 + -0.0 1,025,827 - mgsA b0963 0.10 -0.2 1,025,893 - mgsA b0963 0.25 +1.4 1,025,921 + mgsA b0963 0.31 +1.2 1,025,943 - mgsA b0963 0.36 +2.5 1,025,943 - mgsA b0963 0.36 +1.2 1,026,042 - mgsA b0963 0.57 +0.6 1,026,043 - +0.2 1,026,054 + mgsA b0963 0.60 -0.4 1,026,115 + mgsA b0963 0.73 +0.9 1,026,116 - mgsA b0963 0.73 +1.4 1,026,119 + mgsA b0963 0.74 +0.1 1,026,123 - mgsA b0963 0.75 +0.1 1,026,153 + mgsA b0963 0.81 +2.4 1,026,153 + mgsA b0963 0.81 +1.4 1,026,295 + -2.5 1,026,403 - yccT b0964 0.10 +0.9 1,026,403 - yccT b0964 0.10 -0.8 1,026,627 + yccT b0964 0.44 +0.4 1,026,627 + +2.2 1,026,685 + yccT b0964 0.53 +1.6 1,026,740 + yccT b0964 0.61 +1.2 1,026,740 + yccT b0964 0.61 +2.5
Or see this region's nucleotide sequence