Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pepN and ssuB are separated by 42 nucleotides ssuB and ycbM overlap by 4 nucleotides ycbM and ssuD are separated by 10 nucleotides
b0932: pepN - aminopeptidase N (NCBI), at 989,845 to 992,457
pepN
b0933: ssuB - alkanesulfonate transporter subunit (NCBI), at 992,500 to 993,267
ssuB
b0934: ycbM - putative transport system permease protein (VIMSS), at 993,264 to 994,055
ycbM
b0935: ssuD - alkanesulfonate monooxygenase (NCBI), at 994,066 to 995,211
ssuD
Position (kb) 992
993
994 Strain fitness (log2 ratio) -2
-1
0
1
2 at 991.786 kb on - strand, within pepN at 991.816 kb on + strand, within pepN at 991.847 kb on + strand, within pepN at 991.847 kb on + strand, within pepN at 991.892 kb on - strand, within pepN at 991.892 kb on - strand, within pepN at 991.968 kb on + strand, within pepN at 992.043 kb on - strand, within pepN at 992.045 kb on - strand, within pepN at 992.135 kb on - strand, within pepN at 992.149 kb on + strand, within pepN at 992.402 kb on - strand at 992.438 kb on + strand at 992.582 kb on - strand, within ssuB at 992.676 kb on + strand, within ssuB at 992.681 kb on + strand, within ssuB at 992.712 kb on + strand, within ssuB at 992.928 kb on - strand, within ssuB at 993.003 kb on + strand, within ssuB at 993.003 kb on + strand, within ssuB at 993.056 kb on - strand, within ssuB at 993.078 kb on - strand, within ssuB at 993.103 kb on + strand, within ssuB at 993.239 kb on - strand at 993.526 kb on + strand, within ycbM at 993.729 kb on - strand, within ycbM at 993.913 kb on + strand, within ycbM at 994.024 kb on + strand at 994.033 kb on - strand at 994.089 kb on + strand at 994.089 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 991,786 - pepN b0932 0.74 -0.6 991,816 + pepN b0932 0.75 -0.6 991,847 + pepN b0932 0.77 -1.8 991,847 + pepN b0932 0.77 +0.2 991,892 - pepN b0932 0.78 -0.9 991,892 - pepN b0932 0.78 -1.7 991,968 + pepN b0932 0.81 +1.2 992,043 - pepN b0932 0.84 -0.3 992,045 - pepN b0932 0.84 -0.5 992,135 - pepN b0932 0.88 +2.0 992,149 + pepN b0932 0.88 +0.1 992,402 - -0.8 992,438 + +0.2 992,582 - ssuB b0933 0.11 +0.4 992,676 + ssuB b0933 0.23 -0.9 992,681 + ssuB b0933 0.24 -0.4 992,712 + ssuB b0933 0.28 -2.4 992,928 - ssuB b0933 0.56 +0.2 993,003 + ssuB b0933 0.65 -1.4 993,003 + ssuB b0933 0.65 -2.2 993,056 - ssuB b0933 0.72 +0.2 993,078 - ssuB b0933 0.75 +1.7 993,103 + ssuB b0933 0.79 +1.4 993,239 - +0.9 993,526 + ycbM b0934 0.33 -1.9 993,729 - ycbM b0934 0.59 -1.6 993,913 + ycbM b0934 0.82 +0.5 994,024 + -0.9 994,033 - +0.5 994,089 + +0.9 994,089 + +0.2
Or see this region's nucleotide sequence