Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lpxK and ycaQ are separated by 36 nucleotides ycaQ and ycaR are separated by 51 nucleotides ycaR and kdsB overlap by 4 nucleotides
b0915: lpxK - tetraacyldisaccharide 4'-kinase (NCBI), at 967,589 to 968,575
lpxK
b0916: ycaQ - hypothetical protein (NCBI), at 968,612 to 969,844
ycaQ
b0917: ycaR - hypothetical protein (NCBI), at 969,896 to 970,078
ycaR
b0918: kdsB - 3-deoxy-manno-octulosonate cytidylyltransferase (NCBI), at 970,075 to 970,821
kdsB
Position (kb) 968
969
970 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 968.649 kb on + strand at 968.674 kb on + strand at 968.737 kb on - strand, within ycaQ at 968.737 kb on - strand, within ycaQ at 968.775 kb on + strand, within ycaQ at 968.775 kb on + strand, within ycaQ at 968.811 kb on + strand, within ycaQ at 968.811 kb on + strand at 968.863 kb on - strand, within ycaQ at 968.865 kb on - strand, within ycaQ at 968.880 kb on + strand, within ycaQ at 968.880 kb on + strand, within ycaQ at 969.033 kb on - strand, within ycaQ at 969.050 kb on + strand, within ycaQ at 969.060 kb on - strand, within ycaQ at 969.275 kb on - strand, within ycaQ at 969.282 kb on + strand, within ycaQ at 969.300 kb on - strand, within ycaQ at 969.300 kb on - strand, within ycaQ at 969.547 kb on + strand, within ycaQ at 969.547 kb on + strand, within ycaQ at 969.550 kb on + strand, within ycaQ at 969.555 kb on - strand, within ycaQ at 969.690 kb on + strand, within ycaQ at 969.690 kb on + strand, within ycaQ at 969.774 kb on + strand at 969.830 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 968,649 + +2.2 968,674 + -1.6 968,737 - ycaQ b0916 0.10 +0.5 968,737 - ycaQ b0916 0.10 +3.1 968,775 + ycaQ b0916 0.13 +0.6 968,775 + ycaQ b0916 0.13 +1.1 968,811 + ycaQ b0916 0.16 -1.4 968,811 + -0.4 968,863 - ycaQ b0916 0.20 +1.2 968,865 - ycaQ b0916 0.21 +2.5 968,880 + ycaQ b0916 0.22 -1.8 968,880 + ycaQ b0916 0.22 +2.1 969,033 - ycaQ b0916 0.34 -0.6 969,050 + ycaQ b0916 0.36 -0.4 969,060 - ycaQ b0916 0.36 -0.2 969,275 - ycaQ b0916 0.54 +2.6 969,282 + ycaQ b0916 0.54 +4.7 969,300 - ycaQ b0916 0.56 +1.4 969,300 - ycaQ b0916 0.56 +0.2 969,547 + ycaQ b0916 0.76 -1.4 969,547 + ycaQ b0916 0.76 -0.6 969,550 + ycaQ b0916 0.76 -0.7 969,555 - ycaQ b0916 0.76 +1.0 969,690 + ycaQ b0916 0.87 -0.4 969,690 + ycaQ b0916 0.87 +1.2 969,774 + +1.2 969,830 - +0.7
Or see this region's nucleotide sequence