Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dmsA and dmsB are separated by 10 nucleotides dmsB and dmsC are separated by 1 nucleotides dmsC and ycaC are separated by 34 nucleotides ycaC and ycaD are separated by 313 nucleotides
b0894: dmsA - anaerobic dimethyl sulfoxide reductase subunit A (VIMSS), at 940,182 to 942,626
dmsA
b0895: dmsB - dimethyl sulfoxide reductase, anaerobic, subunit B (NCBI), at 942,637 to 943,254
dmsB
b0896: dmsC - dimethyl sulfoxide reductase, anaerobic, subunit C (NCBI), at 943,256 to 944,119
dmsC
b0897: ycaC - predicted hydrolase (NCBI), at 944,154 to 944,780
ycaC
b0898: ycaD - putative MFS family transporter protein (NCBI), at 945,094 to 946,242
ycaD
Position (kb) 943
944
945 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 942.269 kb on + strand, within dmsA at 942.432 kb on + strand at 942.485 kb on + strand at 942.485 kb on + strand at 942.493 kb on - strand at 942.501 kb on - strand at 942.626 kb on - strand at 942.693 kb on - strand at 942.850 kb on + strand, within dmsB at 942.860 kb on + strand, within dmsB at 942.863 kb on - strand, within dmsB at 942.971 kb on - strand, within dmsB at 943.027 kb on - strand, within dmsB at 943.027 kb on - strand, within dmsB at 943.107 kb on - strand, within dmsB at 943.182 kb on + strand, within dmsB at 943.319 kb on - strand at 943.319 kb on - strand at 943.348 kb on + strand, within dmsC at 943.348 kb on + strand, within dmsC at 943.348 kb on + strand, within dmsC at 943.412 kb on - strand, within dmsC at 943.412 kb on - strand, within dmsC at 943.485 kb on + strand, within dmsC at 943.661 kb on + strand, within dmsC at 943.663 kb on + strand, within dmsC at 943.663 kb on - strand, within dmsC at 943.669 kb on - strand, within dmsC at 943.672 kb on + strand, within dmsC at 943.680 kb on - strand, within dmsC at 943.682 kb on + strand, within dmsC at 943.732 kb on + strand, within dmsC at 943.758 kb on + strand, within dmsC at 943.833 kb on - strand, within dmsC at 943.833 kb on - strand, within dmsC at 943.878 kb on - strand, within dmsC at 943.910 kb on - strand, within dmsC at 943.977 kb on - strand, within dmsC at 943.985 kb on - strand, within dmsC at 944.080 kb on - strand at 944.082 kb on + strand at 944.082 kb on + strand at 944.082 kb on - strand at 944.086 kb on + strand at 944.093 kb on + strand at 944.101 kb on - strand at 944.105 kb on + strand at 944.105 kb on + strand at 944.121 kb on + strand at 944.286 kb on - strand, within ycaC at 944.286 kb on - strand, within ycaC at 944.287 kb on - strand, within ycaC at 944.287 kb on - strand, within ycaC at 944.291 kb on - strand, within ycaC at 944.291 kb on - strand, within ycaC at 944.300 kb on - strand, within ycaC at 944.536 kb on + strand, within ycaC at 944.536 kb on + strand, within ycaC at 944.536 kb on + strand, within ycaC at 944.536 kb on + strand, within ycaC at 944.575 kb on - strand, within ycaC at 944.578 kb on + strand, within ycaC at 945.014 kb on + strand at 945.058 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 942,269 + dmsA b0894 0.85 +1.5 942,432 + -1.8 942,485 + -0.8 942,485 + +1.8 942,493 - -1.2 942,501 - +1.0 942,626 - -1.1 942,693 - +0.0 942,850 + dmsB b0895 0.34 +1.4 942,860 + dmsB b0895 0.36 +1.4 942,863 - dmsB b0895 0.37 +0.2 942,971 - dmsB b0895 0.54 -0.1 943,027 - dmsB b0895 0.63 -0.8 943,027 - dmsB b0895 0.63 -0.1 943,107 - dmsB b0895 0.76 +1.8 943,182 + dmsB b0895 0.88 -2.2 943,319 - +2.8 943,319 - -1.2 943,348 + dmsC b0896 0.11 -0.6 943,348 + dmsC b0896 0.11 -1.1 943,348 + dmsC b0896 0.11 -0.9 943,412 - dmsC b0896 0.18 -1.9 943,412 - dmsC b0896 0.18 +0.1 943,485 + dmsC b0896 0.27 -0.4 943,661 + dmsC b0896 0.47 +1.2 943,663 + dmsC b0896 0.47 -1.1 943,663 - dmsC b0896 0.47 +2.0 943,669 - dmsC b0896 0.48 +1.5 943,672 + dmsC b0896 0.48 -0.6 943,680 - dmsC b0896 0.49 -1.2 943,682 + dmsC b0896 0.49 +0.7 943,732 + dmsC b0896 0.55 -2.1 943,758 + dmsC b0896 0.58 +1.0 943,833 - dmsC b0896 0.67 +0.7 943,833 - dmsC b0896 0.67 -1.5 943,878 - dmsC b0896 0.72 -1.2 943,910 - dmsC b0896 0.76 -2.1 943,977 - dmsC b0896 0.83 +0.2 943,985 - dmsC b0896 0.84 -0.1 944,080 - -1.4 944,082 + -0.4 944,082 + -0.9 944,082 - -0.5 944,086 + -0.9 944,093 + -0.3 944,101 - -1.0 944,105 + +1.2 944,105 + -0.8 944,121 + +1.3 944,286 - ycaC b0897 0.21 -0.4 944,286 - ycaC b0897 0.21 +2.2 944,287 - ycaC b0897 0.21 +2.6 944,287 - ycaC b0897 0.21 -0.8 944,291 - ycaC b0897 0.22 -0.4 944,291 - ycaC b0897 0.22 +0.6 944,300 - ycaC b0897 0.23 +1.6 944,536 + ycaC b0897 0.61 -0.8 944,536 + ycaC b0897 0.61 +2.0 944,536 + ycaC b0897 0.61 -0.6 944,536 + ycaC b0897 0.61 -1.2 944,575 - ycaC b0897 0.67 +1.7 944,578 + ycaC b0897 0.68 +1.9 945,014 + -0.0 945,058 - -0.8
Or see this region's nucleotide sequence