Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt serW and infA are separated by 253 nucleotides infA and aat are separated by 284 nucleotides aat and cydC are separated by 41 nucleotides
b0883: serW - tRNA-Ser (NCBI), at 925,107 to 925,194
serW
b0884: infA - translation initiation factor IF-1 (NCBI), at 925,448 to 925,666
infA
b0885: aat - leucyl/phenylalanyl-tRNA--protein transferase (NCBI), at 925,951 to 926,655
aat
b0886: cydC - fused cysteine transporter subunits of ABC superfamily: membrane component/ATP-binding component (NCBI), at 926,697 to 928,418
cydC
Position (kb) 925
926
927 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 924.954 kb on + strand at 925.246 kb on - strand at 925.246 kb on - strand at 925.319 kb on + strand at 925.319 kb on + strand at 925.319 kb on + strand at 925.327 kb on - strand at 925.686 kb on - strand at 925.687 kb on - strand at 925.687 kb on - strand at 925.779 kb on - strand at 925.885 kb on + strand at 925.967 kb on + strand at 925.967 kb on + strand at 926.089 kb on + strand, within aat at 926.206 kb on + strand, within aat at 926.258 kb on + strand, within aat at 926.299 kb on + strand, within aat at 926.361 kb on - strand, within aat at 926.423 kb on + strand, within aat at 926.453 kb on - strand, within aat at 926.479 kb on + strand, within aat at 926.693 kb on - strand at 926.717 kb on + strand at 926.730 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 924,954 + -0.4 925,246 - -1.6 925,246 - +0.2 925,319 + +0.2 925,319 + +0.8 925,319 + +1.1 925,327 - -1.0 925,686 - -1.1 925,687 - -0.4 925,687 - +0.9 925,779 - +1.5 925,885 + -1.9 925,967 + +0.5 925,967 + -1.2 926,089 + aat b0885 0.20 -1.0 926,206 + aat b0885 0.36 -0.1 926,258 + aat b0885 0.44 +0.4 926,299 + aat b0885 0.49 +2.0 926,361 - aat b0885 0.58 +0.3 926,423 + aat b0885 0.67 +1.7 926,453 - aat b0885 0.71 -0.4 926,479 + aat b0885 0.75 +3.0 926,693 - +1.3 926,717 + +1.2 926,730 - +0.2
Or see this region's nucleotide sequence