Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt artQ and artI are separated by 6 nucleotides artI and artP are separated by 17 nucleotides artP and ybjP are separated by 217 nucleotides ybjP and ybjQ are separated by 125 nucleotides
b0862: artQ - arginine transporter subunit (NCBI), at 900,757 to 901,473
artQ
b0863: artI - arginine transporter subunit (NCBI), at 901,480 to 902,211
artI
b0864: artP - arginine transporter subunit (NCBI), at 902,229 to 902,957
artP
b0865: ybjP - predicted lipoprotein (NCBI), at 903,175 to 903,690
ybjP
b0866: ybjQ - hypothetical protein (NCBI), at 903,816 to 904,139
ybjQ
Position (kb) 902
903 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 901.555 kb on - strand, within artI at 901.590 kb on - strand, within artI at 901.590 kb on - strand, within artI at 901.669 kb on - strand, within artI at 901.669 kb on - strand, within artI at 901.850 kb on + strand, within artI at 901.850 kb on + strand, within artI at 901.858 kb on + strand, within artI at 901.858 kb on - strand, within artI at 901.907 kb on + strand, within artI at 902.066 kb on - strand, within artI at 902.123 kb on - strand, within artI at 902.123 kb on - strand, within artI at 902.289 kb on + strand at 902.302 kb on - strand, within artP at 902.500 kb on - strand, within artP at 902.632 kb on + strand, within artP at 902.632 kb on + strand, within artP at 902.674 kb on + strand, within artP at 902.940 kb on + strand at 902.940 kb on + strand at 902.972 kb on - strand at 903.109 kb on + strand at 903.109 kb on + strand at 903.275 kb on + strand, within ybjP at 903.299 kb on + strand, within ybjP at 903.473 kb on + strand, within ybjP at 903.473 kb on + strand, within ybjP at 903.597 kb on - strand, within ybjP at 903.597 kb on - strand, within ybjP at 903.641 kb on + strand at 903.651 kb on + strand at 903.695 kb on - strand at 903.842 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 901,555 - artI b0863 0.10 +0.4 901,590 - artI b0863 0.15 +0.2 901,590 - artI b0863 0.15 +0.0 901,669 - artI b0863 0.26 +0.2 901,669 - artI b0863 0.26 -1.1 901,850 + artI b0863 0.51 +0.2 901,850 + artI b0863 0.51 +1.2 901,858 + artI b0863 0.52 +0.2 901,858 - artI b0863 0.52 +2.1 901,907 + artI b0863 0.58 +0.8 902,066 - artI b0863 0.80 -1.0 902,123 - artI b0863 0.88 -0.4 902,123 - artI b0863 0.88 -2.1 902,289 + -2.6 902,302 - artP b0864 0.10 -0.8 902,500 - artP b0864 0.37 +1.2 902,632 + artP b0864 0.55 -0.1 902,632 + artP b0864 0.55 -1.8 902,674 + artP b0864 0.61 -0.8 902,940 + -1.2 902,940 + -2.4 902,972 - -1.2 903,109 + +2.9 903,109 + -1.5 903,275 + ybjP b0865 0.19 +0.4 903,299 + ybjP b0865 0.24 +0.7 903,473 + ybjP b0865 0.58 -3.1 903,473 + ybjP b0865 0.58 +0.2 903,597 - ybjP b0865 0.82 +0.5 903,597 - ybjP b0865 0.82 +0.2 903,641 + -1.6 903,651 + -3.0 903,695 - -0.2 903,842 + -0.4
Or see this region's nucleotide sequence