Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybjH and b0844 are separated by 85 nucleotides b0844 and ybjJ overlap by 1 nucleotides ybjJ and ybjK are separated by 83 nucleotides ybjK and rybB are separated by 16 nucleotides rybB and ybjL are separated by 79 nucleotides
b0843: ybjH - hypothetical protein (NCBI), at 884,169 to 884,453
ybjH
b0844: b0844 - orf, hypothetical protein (VIMSS), at 884,539 to 885,354
b0844
b0845: ybjJ - predicted transporter (NCBI), at 885,354 to 886,562
ybjJ
b0846: ybjK - predicted DNA-binding transcriptional regulator (NCBI), at 886,646 to 887,182
ybjK
b4417: rybB - unknown RNA (NCBI), at 887,199 to 887,277
rybB
b0847: ybjL - hypothetical protein (NCBI), at 887,357 to 889,042
ybjL
Position (kb) 885
886
887 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 884.355 kb on + strand, within ybjH at 884.355 kb on + strand, within ybjH at 884.502 kb on + strand at 884.523 kb on - strand at 884.553 kb on - strand at 884.594 kb on + strand at 884.613 kb on - strand at 884.613 kb on - strand at 884.674 kb on + strand, within b0844 at 884.692 kb on + strand, within b0844 at 884.713 kb on + strand, within b0844 at 884.789 kb on - strand, within b0844 at 884.807 kb on + strand, within b0844 at 884.815 kb on - strand, within b0844 at 884.833 kb on + strand, within b0844 at 884.835 kb on + strand, within b0844 at 884.956 kb on - strand, within b0844 at 884.956 kb on - strand, within b0844 at 885.073 kb on + strand, within b0844 at 885.095 kb on + strand, within b0844 at 885.095 kb on + strand, within b0844 at 885.095 kb on + strand, within b0844 at 885.105 kb on - strand, within b0844 at 885.133 kb on + strand, within b0844 at 885.226 kb on + strand, within b0844 at 885.231 kb on + strand, within b0844 at 885.245 kb on - strand, within b0844 at 885.245 kb on - strand, within b0844 at 885.245 kb on - strand, within b0844 at 885.268 kb on - strand, within b0844 at 885.268 kb on - strand, within b0844 at 885.272 kb on + strand, within b0844 at 885.272 kb on + strand, within b0844 at 885.272 kb on + strand, within b0844 at 885.280 kb on - strand at 885.280 kb on - strand at 885.296 kb on - strand at 885.320 kb on - strand at 885.350 kb on - strand at 885.471 kb on - strand at 885.494 kb on - strand, within ybjJ at 885.533 kb on + strand, within ybjJ at 885.533 kb on + strand, within ybjJ at 885.551 kb on - strand, within ybjJ at 885.626 kb on - strand, within ybjJ at 885.628 kb on + strand, within ybjJ at 885.674 kb on + strand, within ybjJ at 885.674 kb on + strand, within ybjJ at 885.689 kb on - strand, within ybjJ at 885.693 kb on + strand, within ybjJ at 885.706 kb on + strand, within ybjJ at 885.743 kb on + strand, within ybjJ at 885.909 kb on - strand, within ybjJ at 885.951 kb on + strand, within ybjJ at 886.005 kb on - strand, within ybjJ at 886.291 kb on + strand, within ybjJ at 886.291 kb on + strand, within ybjJ at 886.313 kb on + strand, within ybjJ at 886.363 kb on + strand, within ybjJ at 886.395 kb on - strand, within ybjJ at 886.430 kb on + strand, within ybjJ at 886.457 kb on + strand at 886.522 kb on - strand at 886.522 kb on - strand at 886.814 kb on - strand, within ybjK at 886.815 kb on - strand, within ybjK at 886.815 kb on - strand, within ybjK at 886.822 kb on + strand, within ybjK at 886.822 kb on + strand, within ybjK at 886.840 kb on + strand, within ybjK at 886.887 kb on - strand, within ybjK at 886.887 kb on - strand, within ybjK at 886.926 kb on - strand, within ybjK at 886.977 kb on - strand, within ybjK at 887.034 kb on + strand, within ybjK at 887.034 kb on + strand, within ybjK at 887.034 kb on + strand, within ybjK at 887.169 kb on - strand at 887.169 kb on - strand at 887.170 kb on - strand at 887.286 kb on - strand at 887.292 kb on + strand at 887.399 kb on - strand at 887.399 kb on - strand at 887.399 kb on - strand at 887.418 kb on - strand at 887.441 kb on + strand at 887.442 kb on - strand at 887.449 kb on - strand at 887.449 kb on - strand at 887.515 kb on + strand at 887.554 kb on + strand, within ybjL
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 884,355 + ybjH b0843 0.65 -1.8 884,355 + ybjH b0843 0.65 +1.5 884,502 + -0.4 884,523 - -1.3 884,553 - +1.2 884,594 + +1.5 884,613 - +1.8 884,613 - -1.8 884,674 + b0844 b0844 0.17 +0.1 884,692 + b0844 b0844 0.19 +2.0 884,713 + b0844 b0844 0.21 -1.9 884,789 - b0844 b0844 0.31 -0.7 884,807 + b0844 b0844 0.33 +1.3 884,815 - b0844 b0844 0.34 -0.9 884,833 + b0844 b0844 0.36 -0.2 884,835 + b0844 b0844 0.36 -0.4 884,956 - b0844 b0844 0.51 +0.2 884,956 - b0844 b0844 0.51 -1.1 885,073 + b0844 b0844 0.65 +0.2 885,095 + b0844 b0844 0.68 +0.1 885,095 + b0844 b0844 0.68 -2.5 885,095 + b0844 b0844 0.68 +2.4 885,105 - b0844 b0844 0.69 -0.7 885,133 + b0844 b0844 0.73 +0.7 885,226 + b0844 b0844 0.84 +1.2 885,231 + b0844 b0844 0.85 -0.2 885,245 - b0844 b0844 0.87 -2.2 885,245 - b0844 b0844 0.87 +0.8 885,245 - b0844 b0844 0.87 -0.1 885,268 - b0844 b0844 0.89 +0.9 885,268 - b0844 b0844 0.89 +0.1 885,272 + b0844 b0844 0.90 +1.5 885,272 + b0844 b0844 0.90 +0.1 885,272 + b0844 b0844 0.90 -2.8 885,280 - -1.4 885,280 - -0.7 885,296 - -0.4 885,320 - -1.4 885,350 - -1.6 885,471 - +0.9 885,494 - ybjJ b0845 0.12 +2.2 885,533 + ybjJ b0845 0.15 -1.8 885,533 + ybjJ b0845 0.15 -1.4 885,551 - ybjJ b0845 0.16 -0.6 885,626 - ybjJ b0845 0.22 -1.0 885,628 + ybjJ b0845 0.23 -1.2 885,674 + ybjJ b0845 0.26 -2.4 885,674 + ybjJ b0845 0.26 +0.7 885,689 - ybjJ b0845 0.28 -1.4 885,693 + ybjJ b0845 0.28 +0.8 885,706 + ybjJ b0845 0.29 -0.2 885,743 + ybjJ b0845 0.32 -1.2 885,909 - ybjJ b0845 0.46 -0.2 885,951 + ybjJ b0845 0.49 -2.2 886,005 - ybjJ b0845 0.54 +0.8 886,291 + ybjJ b0845 0.78 +0.9 886,291 + ybjJ b0845 0.78 -1.9 886,313 + ybjJ b0845 0.79 -1.0 886,363 + ybjJ b0845 0.83 -0.5 886,395 - ybjJ b0845 0.86 -0.9 886,430 + ybjJ b0845 0.89 +1.6 886,457 + +2.2 886,522 - +0.4 886,522 - +0.0 886,814 - ybjK b0846 0.31 +2.1 886,815 - ybjK b0846 0.31 +2.2 886,815 - ybjK b0846 0.31 +2.7 886,822 + ybjK b0846 0.33 +1.7 886,822 + ybjK b0846 0.33 +1.1 886,840 + ybjK b0846 0.36 -0.4 886,887 - ybjK b0846 0.45 +1.8 886,887 - ybjK b0846 0.45 +0.4 886,926 - ybjK b0846 0.52 +2.8 886,977 - ybjK b0846 0.62 +1.2 887,034 + ybjK b0846 0.72 +2.8 887,034 + ybjK b0846 0.72 +0.6 887,034 + ybjK b0846 0.72 +1.4 887,169 - -0.4 887,169 - +5.4 887,170 - -2.2 887,286 - -1.7 887,292 + +2.2 887,399 - +0.4 887,399 - -1.1 887,399 - -1.6 887,418 - -0.8 887,441 + -0.2 887,442 - -2.8 887,449 - -0.8 887,449 - -2.2 887,515 + -1.6 887,554 + ybjL b0847 0.12 -1.1
Or see this region's nucleotide sequence