Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybhO and ybhP overlap by 4 nucleotides ybhP and ybhQ are separated by 132 nucleotides ybhQ and ybhR overlap by 39 nucleotides
b0789: ybhO - cardiolipin synthase 2 (NCBI), at 821,721 to 822,962
ybhO
b0790: ybhP - predicted DNase (NCBI), at 822,959 to 823,720
ybhP
b0791: ybhQ - predicted inner membrane protein (NCBI), at 823,853 to 824,263
ybhQ
b0792: ybhR - predicted transporter subunit: membrane component of ABC superfamily (NCBI), at 824,225 to 825,331
ybhR
Position (kb) 822
823
824 Strain fitness (log2 ratio) -2
-1
0
1
2 at 822.100 kb on + strand, within ybhO at 822.100 kb on + strand, within ybhO at 822.155 kb on - strand, within ybhO at 822.188 kb on - strand, within ybhO at 822.188 kb on - strand, within ybhO at 822.273 kb on + strand, within ybhO at 822.328 kb on - strand, within ybhO at 822.328 kb on - strand, within ybhO at 822.553 kb on + strand, within ybhO at 822.553 kb on + strand, within ybhO at 822.780 kb on + strand, within ybhO at 822.780 kb on + strand, within ybhO at 822.849 kb on + strand at 822.891 kb on - strand at 823.224 kb on + strand, within ybhP at 823.224 kb on + strand, within ybhP at 823.296 kb on - strand, within ybhP at 823.296 kb on - strand, within ybhP at 823.302 kb on - strand, within ybhP at 823.331 kb on + strand, within ybhP at 823.346 kb on + strand, within ybhP at 823.405 kb on + strand, within ybhP at 823.434 kb on - strand, within ybhP at 823.454 kb on - strand, within ybhP at 823.604 kb on - strand, within ybhP at 823.653 kb on - strand at 823.850 kb on + strand at 823.893 kb on - strand at 823.947 kb on + strand, within ybhQ at 823.961 kb on - strand, within ybhQ at 823.993 kb on + strand, within ybhQ at 824.064 kb on - strand, within ybhQ at 824.068 kb on + strand, within ybhQ at 824.288 kb on - strand at 824.354 kb on + strand, within ybhR at 824.354 kb on + strand, within ybhR at 824.371 kb on - strand, within ybhR at 824.371 kb on - strand, within ybhR at 824.501 kb on + strand, within ybhR at 824.559 kb on + strand, within ybhR at 824.621 kb on - strand, within ybhR at 824.689 kb on - strand, within ybhR
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 822,100 + ybhO b0789 0.31 +1.9 822,100 + ybhO b0789 0.31 +2.5 822,155 - ybhO b0789 0.35 +1.0 822,188 - ybhO b0789 0.38 -0.8 822,188 - ybhO b0789 0.38 +1.5 822,273 + ybhO b0789 0.44 -1.8 822,328 - ybhO b0789 0.49 +1.2 822,328 - ybhO b0789 0.49 -1.7 822,553 + ybhO b0789 0.67 -0.4 822,553 + ybhO b0789 0.67 -0.4 822,780 + ybhO b0789 0.85 -0.8 822,780 + ybhO b0789 0.85 -1.0 822,849 + -0.7 822,891 - +0.6 823,224 + ybhP b0790 0.35 +0.2 823,224 + ybhP b0790 0.35 +1.2 823,296 - ybhP b0790 0.44 -1.2 823,296 - ybhP b0790 0.44 +2.7 823,302 - ybhP b0790 0.45 +0.8 823,331 + ybhP b0790 0.49 +0.9 823,346 + ybhP b0790 0.51 +1.3 823,405 + ybhP b0790 0.59 -1.8 823,434 - ybhP b0790 0.62 +2.0 823,454 - ybhP b0790 0.65 +0.7 823,604 - ybhP b0790 0.85 +0.0 823,653 - +1.1 823,850 + -1.5 823,893 - +0.4 823,947 + ybhQ b0791 0.23 -1.5 823,961 - ybhQ b0791 0.26 +0.7 823,993 + ybhQ b0791 0.34 +0.2 824,064 - ybhQ b0791 0.51 -0.8 824,068 + ybhQ b0791 0.52 +0.4 824,288 - -0.2 824,354 + ybhR b0792 0.12 -2.4 824,354 + ybhR b0792 0.12 -1.8 824,371 - ybhR b0792 0.13 +0.2 824,371 - ybhR b0792 0.13 +0.4 824,501 + ybhR b0792 0.25 +1.2 824,559 + ybhR b0792 0.30 -2.5 824,621 - ybhR b0792 0.36 -1.9 824,689 - ybhR b0792 0.42 +1.2
Or see this region's nucleotide sequence