Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt moaA and moaB are separated by 21 nucleotides moaB and moaC are separated by 2 nucleotides moaC and moaD overlap by 8 nucleotides moaD and moaE are separated by 1 nucleotides
b0781: moaA - molybdenum cofactor biosynthesis protein A (NCBI), at 816,267 to 817,256
moaA
b0782: moaB - molybdopterin biosynthesis protein B (NCBI), at 817,278 to 817,790
moaB
b0783: moaC - molybdenum cofactor biosynthesis protein C (NCBI), at 817,793 to 818,278
moaC
b0784: moaD - molybdopterin synthase, small subunit (NCBI), at 818,271 to 818,516
moaD
b0785: moaE - molybdopterin synthase, large subunit (NCBI), at 818,518 to 818,970
moaE
Position (kb) 817
818 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 816.333 kb on - strand at 816.363 kb on + strand at 816.363 kb on + strand at 816.378 kb on - strand, within moaA at 816.609 kb on - strand, within moaA at 816.624 kb on + strand, within moaA at 816.680 kb on + strand, within moaA at 816.746 kb on + strand, within moaA at 816.746 kb on + strand, within moaA at 816.782 kb on - strand, within moaA at 816.817 kb on + strand, within moaA at 816.912 kb on + strand, within moaA at 817.009 kb on - strand, within moaA at 817.009 kb on - strand, within moaA at 817.049 kb on - strand, within moaA at 817.085 kb on - strand, within moaA at 817.105 kb on + strand, within moaA at 817.105 kb on + strand, within moaA at 817.105 kb on + strand, within moaA at 817.280 kb on - strand at 817.280 kb on - strand at 817.355 kb on - strand, within moaB at 817.485 kb on + strand, within moaB at 817.485 kb on + strand, within moaB at 817.818 kb on - strand at 817.818 kb on - strand at 817.852 kb on - strand, within moaC at 817.852 kb on - strand, within moaC at 817.872 kb on - strand, within moaC at 817.912 kb on + strand, within moaC at 817.912 kb on + strand, within moaC at 818.006 kb on - strand, within moaC at 818.012 kb on - strand, within moaC at 818.167 kb on - strand, within moaC at 818.211 kb on + strand, within moaC at 818.219 kb on - strand, within moaC at 818.219 kb on - strand, within moaC at 818.252 kb on + strand at 818.252 kb on + strand at 818.369 kb on + strand, within moaD at 818.455 kb on + strand, within moaD at 818.455 kb on + strand, within moaD at 818.566 kb on - strand, within moaE at 818.566 kb on - strand, within moaE at 818.566 kb on - strand, within moaE at 818.677 kb on - strand, within moaE at 818.687 kb on - strand, within moaE at 818.764 kb on - strand, within moaE
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 816,333 - +0.4 816,363 + +0.1 816,363 + +2.0 816,378 - moaA b0781 0.11 -0.4 816,609 - moaA b0781 0.35 +2.1 816,624 + moaA b0781 0.36 +1.5 816,680 + moaA b0781 0.42 +1.8 816,746 + moaA b0781 0.48 +1.5 816,746 + moaA b0781 0.48 +0.7 816,782 - moaA b0781 0.52 -0.1 816,817 + moaA b0781 0.56 +3.7 816,912 + moaA b0781 0.65 +1.6 817,009 - moaA b0781 0.75 +1.7 817,009 - moaA b0781 0.75 -0.2 817,049 - moaA b0781 0.79 +2.2 817,085 - moaA b0781 0.83 +3.7 817,105 + moaA b0781 0.85 -0.9 817,105 + moaA b0781 0.85 +0.6 817,105 + moaA b0781 0.85 -0.2 817,280 - -0.1 817,280 - +1.6 817,355 - moaB b0782 0.15 +1.9 817,485 + moaB b0782 0.40 +2.3 817,485 + moaB b0782 0.40 +2.6 817,818 - +1.6 817,818 - +2.1 817,852 - moaC b0783 0.12 +2.1 817,852 - moaC b0783 0.12 -0.9 817,872 - moaC b0783 0.16 -0.9 817,912 + moaC b0783 0.24 -0.9 817,912 + moaC b0783 0.24 -0.9 818,006 - moaC b0783 0.44 +2.8 818,012 - moaC b0783 0.45 -0.2 818,167 - moaC b0783 0.77 +0.8 818,211 + moaC b0783 0.86 -2.3 818,219 - moaC b0783 0.88 +2.1 818,219 - moaC b0783 0.88 +1.6 818,252 + +0.3 818,252 + +1.1 818,369 + moaD b0784 0.40 +2.1 818,455 + moaD b0784 0.75 +0.1 818,455 + moaD b0784 0.75 -1.9 818,566 - moaE b0785 0.11 +0.4 818,566 - moaE b0785 0.11 +1.2 818,566 - moaE b0785 0.11 +1.6 818,677 - moaE b0785 0.35 +0.8 818,687 - moaE b0785 0.37 -0.4 818,764 - moaE b0785 0.54 +2.2
Or see this region's nucleotide sequence