Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybgL and nei are separated by 35 nucleotides nei and abrB overlap by 4 nucleotides abrB and ybgO are separated by 151 nucleotides
b0713: ybgL - hypothetical protein (NCBI), at 744,388 to 745,122
ybgL
b0714: nei - endonuclease VIII/ 5-formyluracil/5-hydroxymethyluracil DNA glycosylase (NCBI), at 745,158 to 745,949
nei
b0715: abrB - putative transport protein (VIMSS), at 745,946 to 746,992
abrB
b0716: ybgO - orf, hypothetical protein (VIMSS), at 747,144 to 748,205
ybgO
Position (kb) 745
746
747 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 744.978 kb on - strand, within ybgL at 744.978 kb on - strand, within ybgL at 745.140 kb on - strand at 745.148 kb on + strand at 745.194 kb on + strand at 745.194 kb on + strand at 745.286 kb on + strand, within nei at 745.297 kb on + strand, within nei at 745.297 kb on + strand, within nei at 745.358 kb on - strand, within nei at 745.358 kb on - strand, within nei at 745.419 kb on + strand, within nei at 745.689 kb on - strand, within nei at 745.991 kb on + strand at 746.028 kb on - strand at 746.035 kb on - strand at 746.088 kb on + strand, within abrB at 746.122 kb on - strand, within abrB at 746.218 kb on - strand, within abrB at 746.267 kb on - strand, within abrB at 746.335 kb on - strand, within abrB at 746.335 kb on - strand, within abrB at 746.412 kb on - strand, within abrB at 746.422 kb on - strand, within abrB at 746.509 kb on + strand, within abrB at 746.651 kb on - strand, within abrB at 746.768 kb on + strand, within abrB at 746.768 kb on + strand, within abrB at 746.815 kb on - strand, within abrB at 746.869 kb on + strand, within abrB at 746.929 kb on - strand at 747.003 kb on - strand at 747.003 kb on - strand at 747.024 kb on + strand at 747.027 kb on + strand at 747.042 kb on - strand at 747.172 kb on + strand at 747.172 kb on + strand at 747.251 kb on + strand, within ybgO at 747.251 kb on + strand, within ybgO at 747.255 kb on - strand, within ybgO at 747.255 kb on - strand, within ybgO at 747.303 kb on - strand, within ybgO at 747.329 kb on + strand, within ybgO at 747.346 kb on + strand, within ybgO at 747.363 kb on - strand, within ybgO at 747.363 kb on - strand, within ybgO at 747.372 kb on - strand, within ybgO at 747.372 kb on - strand, within ybgO at 747.382 kb on - strand, within ybgO at 747.382 kb on - strand, within ybgO at 747.393 kb on + strand, within ybgO at 747.393 kb on - strand, within ybgO at 747.393 kb on - strand, within ybgO at 747.393 kb on - strand, within ybgO at 747.435 kb on + strand, within ybgO at 747.438 kb on - strand, within ybgO at 747.438 kb on - strand, within ybgO at 747.446 kb on + strand, within ybgO at 747.446 kb on + strand, within ybgO at 747.470 kb on - strand, within ybgO at 747.470 kb on - strand, within ybgO at 747.554 kb on + strand, within ybgO at 747.583 kb on + strand, within ybgO at 747.583 kb on + strand, within ybgO at 747.618 kb on - strand, within ybgO at 747.618 kb on - strand, within ybgO at 747.619 kb on + strand, within ybgO at 747.716 kb on + strand, within ybgO at 747.724 kb on - strand, within ybgO at 747.724 kb on - strand, within ybgO at 747.760 kb on + strand, within ybgO at 747.760 kb on + strand, within ybgO at 747.804 kb on - strand, within ybgO at 747.806 kb on + strand, within ybgO at 747.814 kb on - strand, within ybgO at 747.819 kb on - strand, within ybgO at 747.819 kb on - strand, within ybgO at 747.824 kb on - strand, within ybgO at 747.837 kb on + strand, within ybgO at 747.838 kb on + strand, within ybgO at 747.838 kb on + strand, within ybgO at 747.841 kb on + strand, within ybgO at 747.846 kb on - strand, within ybgO at 747.897 kb on + strand, within ybgO at 747.897 kb on + strand at 747.957 kb on + strand, within ybgO at 747.957 kb on + strand, within ybgO at 747.957 kb on + strand, within ybgO at 747.958 kb on + strand, within ybgO at 747.960 kb on - strand, within ybgO
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 744,978 - ybgL b0713 0.80 -0.9 744,978 - ybgL b0713 0.80 +1.2 745,140 - +3.6 745,148 + -1.4 745,194 + +0.5 745,194 + -1.4 745,286 + nei b0714 0.16 +1.3 745,297 + nei b0714 0.18 +1.2 745,297 + nei b0714 0.18 +0.4 745,358 - nei b0714 0.25 +1.9 745,358 - nei b0714 0.25 +2.6 745,419 + nei b0714 0.33 +0.5 745,689 - nei b0714 0.67 -0.4 745,991 + -2.4 746,028 - +0.2 746,035 - +0.9 746,088 + abrB b0715 0.14 -2.7 746,122 - abrB b0715 0.17 +0.1 746,218 - abrB b0715 0.26 -0.1 746,267 - abrB b0715 0.31 +1.5 746,335 - abrB b0715 0.37 +0.9 746,335 - abrB b0715 0.37 +0.4 746,412 - abrB b0715 0.45 -0.6 746,422 - abrB b0715 0.45 +0.3 746,509 + abrB b0715 0.54 -0.6 746,651 - abrB b0715 0.67 +0.3 746,768 + abrB b0715 0.79 -0.4 746,768 + abrB b0715 0.79 +3.5 746,815 - abrB b0715 0.83 +1.3 746,869 + abrB b0715 0.88 +1.2 746,929 - +0.7 747,003 - -0.4 747,003 - +1.8 747,024 + -2.4 747,027 + +1.4 747,042 - +0.5 747,172 + -0.2 747,172 + +1.0 747,251 + ybgO b0716 0.10 +0.8 747,251 + ybgO b0716 0.10 -1.6 747,255 - ybgO b0716 0.10 -0.4 747,255 - ybgO b0716 0.10 -2.3 747,303 - ybgO b0716 0.15 -0.6 747,329 + ybgO b0716 0.17 -2.1 747,346 + ybgO b0716 0.19 +1.6 747,363 - ybgO b0716 0.21 -1.0 747,363 - ybgO b0716 0.21 -1.3 747,372 - ybgO b0716 0.21 -2.1 747,372 - ybgO b0716 0.21 +0.2 747,382 - ybgO b0716 0.22 -1.1 747,382 - ybgO b0716 0.22 +0.2 747,393 + ybgO b0716 0.23 -1.8 747,393 - ybgO b0716 0.23 -1.1 747,393 - ybgO b0716 0.23 -1.8 747,393 - ybgO b0716 0.23 -1.2 747,435 + ybgO b0716 0.27 -1.4 747,438 - ybgO b0716 0.28 -0.1 747,438 - ybgO b0716 0.28 -0.0 747,446 + ybgO b0716 0.28 -0.5 747,446 + ybgO b0716 0.28 -0.1 747,470 - ybgO b0716 0.31 +0.4 747,470 - ybgO b0716 0.31 +0.2 747,554 + ybgO b0716 0.39 -1.3 747,583 + ybgO b0716 0.41 +1.6 747,583 + ybgO b0716 0.41 +0.8 747,618 - ybgO b0716 0.45 -1.8 747,618 - ybgO b0716 0.45 -1.8 747,619 + ybgO b0716 0.45 +0.6 747,716 + ybgO b0716 0.54 -0.2 747,724 - ybgO b0716 0.55 -1.4 747,724 - ybgO b0716 0.55 -1.7 747,760 + ybgO b0716 0.58 -0.8 747,760 + ybgO b0716 0.58 +1.2 747,804 - ybgO b0716 0.62 -1.4 747,806 + ybgO b0716 0.62 -0.9 747,814 - ybgO b0716 0.63 +1.2 747,819 - ybgO b0716 0.64 -0.8 747,819 - ybgO b0716 0.64 -0.8 747,824 - ybgO b0716 0.64 -0.8 747,837 + ybgO b0716 0.65 -0.3 747,838 + ybgO b0716 0.65 +1.2 747,838 + ybgO b0716 0.65 -0.4 747,841 + ybgO b0716 0.66 +0.8 747,846 - ybgO b0716 0.66 -1.6 747,897 + ybgO b0716 0.71 -0.2 747,897 + +1.2 747,957 + ybgO b0716 0.77 -2.2 747,957 + ybgO b0716 0.77 -0.4 747,957 + ybgO b0716 0.77 -1.1 747,958 + ybgO b0716 0.77 -1.1 747,960 - ybgO b0716 0.77 +0.2
Or see this region's nucleotide sequence