Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybgK and ybgL overlap by 11 nucleotides ybgL and nei are separated by 35 nucleotides nei and abrB overlap by 4 nucleotides
b0712: ybgK - predicted enzyme subunit (NCBI), at 743,466 to 744,398
ybgK
b0713: ybgL - hypothetical protein (NCBI), at 744,388 to 745,122
ybgL
b0714: nei - endonuclease VIII/ 5-formyluracil/5-hydroxymethyluracil DNA glycosylase (NCBI), at 745,158 to 745,949
nei
b0715: abrB - putative transport protein (VIMSS), at 745,946 to 746,992
abrB
Position (kb) 745
746 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 744.271 kb on - strand, within ybgK at 744.423 kb on - strand at 744.662 kb on - strand, within ybgL at 744.662 kb on - strand, within ybgL at 744.711 kb on + strand, within ybgL at 744.766 kb on + strand, within ybgL at 744.832 kb on - strand, within ybgL at 744.978 kb on - strand, within ybgL at 744.978 kb on - strand, within ybgL at 745.140 kb on - strand at 745.148 kb on + strand at 745.194 kb on + strand at 745.194 kb on + strand at 745.286 kb on + strand, within nei at 745.297 kb on + strand, within nei at 745.297 kb on + strand, within nei at 745.358 kb on - strand, within nei at 745.358 kb on - strand, within nei at 745.419 kb on + strand, within nei at 745.689 kb on - strand, within nei at 745.991 kb on + strand at 746.028 kb on - strand at 746.035 kb on - strand at 746.088 kb on + strand, within abrB at 746.122 kb on - strand, within abrB at 746.218 kb on - strand, within abrB at 746.267 kb on - strand, within abrB at 746.335 kb on - strand, within abrB at 746.335 kb on - strand, within abrB at 746.412 kb on - strand, within abrB at 746.422 kb on - strand, within abrB at 746.509 kb on + strand, within abrB at 746.651 kb on - strand, within abrB at 746.768 kb on + strand, within abrB at 746.768 kb on + strand, within abrB at 746.815 kb on - strand, within abrB at 746.869 kb on + strand, within abrB at 746.929 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 744,271 - ybgK b0712 0.86 +0.4 744,423 - -0.8 744,662 - ybgL b0713 0.37 -0.1 744,662 - ybgL b0713 0.37 -1.1 744,711 + ybgL b0713 0.44 -0.5 744,766 + ybgL b0713 0.51 +1.2 744,832 - ybgL b0713 0.60 +0.7 744,978 - ybgL b0713 0.80 -0.9 744,978 - ybgL b0713 0.80 +1.2 745,140 - +3.6 745,148 + -1.4 745,194 + +0.5 745,194 + -1.4 745,286 + nei b0714 0.16 +1.3 745,297 + nei b0714 0.18 +1.2 745,297 + nei b0714 0.18 +0.4 745,358 - nei b0714 0.25 +1.9 745,358 - nei b0714 0.25 +2.6 745,419 + nei b0714 0.33 +0.5 745,689 - nei b0714 0.67 -0.4 745,991 + -2.4 746,028 - +0.2 746,035 - +0.9 746,088 + abrB b0715 0.14 -2.7 746,122 - abrB b0715 0.17 +0.1 746,218 - abrB b0715 0.26 -0.1 746,267 - abrB b0715 0.31 +1.5 746,335 - abrB b0715 0.37 +0.9 746,335 - abrB b0715 0.37 +0.4 746,412 - abrB b0715 0.45 -0.6 746,422 - abrB b0715 0.45 +0.3 746,509 + abrB b0715 0.54 -0.6 746,651 - abrB b0715 0.67 +0.3 746,768 + abrB b0715 0.79 -0.4 746,768 + abrB b0715 0.79 +3.5 746,815 - abrB b0715 0.83 +1.3 746,869 + abrB b0715 0.88 +1.2 746,929 - +0.7
Or see this region's nucleotide sequence