Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybgI and ybgJ are separated by 22 nucleotides ybgJ and ybgK overlap by 7 nucleotides ybgK and ybgL overlap by 11 nucleotides
b0710: ybgI - conserved metal-binding protein (NCBI), at 742,050 to 742,793
ybgI
b0711: ybgJ - predicted enzyme subunit (NCBI), at 742,816 to 743,472
ybgJ
b0712: ybgK - predicted enzyme subunit (NCBI), at 743,466 to 744,398
ybgK
b0713: ybgL - hypothetical protein (NCBI), at 744,388 to 745,122
ybgL
Position (kb) 742
743
744 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 741.831 kb on - strand at 741.987 kb on + strand at 742.010 kb on + strand at 742.010 kb on + strand at 742.019 kb on + strand at 742.126 kb on + strand, within ybgI at 742.191 kb on - strand, within ybgI at 742.191 kb on - strand, within ybgI at 742.292 kb on - strand, within ybgI at 742.357 kb on + strand, within ybgI at 742.357 kb on + strand, within ybgI at 742.438 kb on + strand, within ybgI at 742.541 kb on + strand, within ybgI at 742.541 kb on + strand, within ybgI at 742.615 kb on + strand, within ybgI at 742.699 kb on - strand, within ybgI at 742.753 kb on + strand at 742.761 kb on - strand at 742.761 kb on - strand at 742.973 kb on - strand, within ybgJ at 743.091 kb on + strand, within ybgJ at 743.091 kb on + strand, within ybgJ at 743.091 kb on + strand, within ybgJ at 743.185 kb on - strand, within ybgJ at 743.185 kb on - strand, within ybgJ at 743.185 kb on - strand, within ybgJ at 743.248 kb on - strand, within ybgJ at 743.250 kb on + strand, within ybgJ at 743.250 kb on + strand, within ybgJ at 743.302 kb on + strand, within ybgJ at 743.369 kb on + strand, within ybgJ at 743.374 kb on + strand, within ybgJ at 743.461 kb on + strand at 743.489 kb on + strand at 743.492 kb on + strand at 743.492 kb on + strand at 743.492 kb on + strand at 743.500 kb on - strand at 743.500 kb on - strand at 743.504 kb on + strand at 743.515 kb on - strand at 743.538 kb on + strand at 743.538 kb on + strand at 743.539 kb on + strand at 743.539 kb on + strand at 743.595 kb on - strand, within ybgK at 743.595 kb on - strand, within ybgK at 743.642 kb on + strand, within ybgK at 743.799 kb on + strand, within ybgK at 743.865 kb on - strand, within ybgK at 743.880 kb on + strand, within ybgK at 743.880 kb on + strand, within ybgK at 743.888 kb on - strand, within ybgK at 743.920 kb on - strand, within ybgK at 743.946 kb on - strand, within ybgK at 743.956 kb on + strand, within ybgK at 743.956 kb on + strand, within ybgK at 744.039 kb on + strand, within ybgK at 744.039 kb on + strand, within ybgK at 744.080 kb on + strand, within ybgK at 744.080 kb on + strand, within ybgK at 744.152 kb on + strand, within ybgK at 744.271 kb on - strand, within ybgK at 744.423 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 741,831 - +0.4 741,987 + +0.6 742,010 + -1.4 742,010 + -0.4 742,019 + -2.2 742,126 + ybgI b0710 0.10 -1.1 742,191 - ybgI b0710 0.19 +1.1 742,191 - ybgI b0710 0.19 -2.2 742,292 - ybgI b0710 0.33 -0.5 742,357 + ybgI b0710 0.41 -1.8 742,357 + ybgI b0710 0.41 +3.9 742,438 + ybgI b0710 0.52 -1.4 742,541 + ybgI b0710 0.66 -0.0 742,541 + ybgI b0710 0.66 -1.9 742,615 + ybgI b0710 0.76 -1.4 742,699 - ybgI b0710 0.87 -0.6 742,753 + -1.9 742,761 - -2.7 742,761 - +2.0 742,973 - ybgJ b0711 0.24 -2.7 743,091 + ybgJ b0711 0.42 -1.1 743,091 + ybgJ b0711 0.42 +1.6 743,091 + ybgJ b0711 0.42 +1.9 743,185 - ybgJ b0711 0.56 +0.2 743,185 - ybgJ b0711 0.56 -1.6 743,185 - ybgJ b0711 0.56 +1.5 743,248 - ybgJ b0711 0.66 -0.8 743,250 + ybgJ b0711 0.66 +0.7 743,250 + ybgJ b0711 0.66 +2.1 743,302 + ybgJ b0711 0.74 -1.3 743,369 + ybgJ b0711 0.84 +1.5 743,374 + ybgJ b0711 0.85 -0.8 743,461 + +2.4 743,489 + -2.6 743,492 + +0.4 743,492 + -0.4 743,492 + -1.1 743,500 - +0.9 743,500 - -0.4 743,504 + +1.5 743,515 - -1.0 743,538 + +1.2 743,538 + -0.4 743,539 + +0.2 743,539 + +0.2 743,595 - ybgK b0712 0.14 +1.2 743,595 - ybgK b0712 0.14 +2.6 743,642 + ybgK b0712 0.19 +1.2 743,799 + ybgK b0712 0.36 -1.1 743,865 - ybgK b0712 0.43 -2.0 743,880 + ybgK b0712 0.44 +0.8 743,880 + ybgK b0712 0.44 -0.4 743,888 - ybgK b0712 0.45 +1.2 743,920 - ybgK b0712 0.49 +1.8 743,946 - ybgK b0712 0.51 -0.6 743,956 + ybgK b0712 0.53 +0.2 743,956 + ybgK b0712 0.53 +1.2 744,039 + ybgK b0712 0.61 +1.5 744,039 + ybgK b0712 0.61 -0.4 744,080 + ybgK b0712 0.66 +1.2 744,080 + ybgK b0712 0.66 +0.2 744,152 + ybgK b0712 0.74 +0.8 744,271 - ybgK b0712 0.86 +0.4 744,423 - -0.8
Or see this region's nucleotide sequence