Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cobC and nadD are separated by 23 nucleotides nadD and holA are separated by 1 nucleotides holA and rlpB overlap by 1 nucleotides rlpB and leuS are separated by 14 nucleotides
b0638: cobC - predicted alpha-ribazole-5'-P phosphatase (NCBI), at 668,519 to 669,130
cobC
b0639: nadD - nicotinic acid mononucleotide adenyltransferase (NCBI), at 669,154 to 669,795
nadD
b0640: holA - DNA polymerase III subunit delta (NCBI), at 669,797 to 670,828
holA
b0641: rlpB - minor lipoprotein (NCBI), at 670,828 to 671,409
rlpB
b0642: leuS - leucyl-tRNA synthetase (NCBI), at 671,424 to 674,006
leuS
Position (kb) 669
670
671 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 668.930 kb on + strand, within cobC at 668.948 kb on + strand, within cobC at 668.948 kb on + strand, within cobC at 668.992 kb on + strand, within cobC at 669.031 kb on + strand, within cobC at 669.048 kb on + strand, within cobC at 669.057 kb on - strand, within cobC at 669.057 kb on - strand, within cobC at 669.095 kb on - strand at 669.153 kb on - strand at 669.158 kb on - strand at 669.158 kb on - strand at 669.179 kb on + strand at 669.187 kb on - strand at 669.852 kb on + strand at 670.785 kb on + strand at 670.837 kb on - strand at 670.880 kb on - strand at 670.915 kb on - strand, within rlpB at 670.922 kb on - strand, within rlpB at 671.394 kb on - strand at 671.404 kb on - strand at 671.404 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 668,930 + cobC b0638 0.67 -2.2 668,948 + cobC b0638 0.70 +1.2 668,948 + cobC b0638 0.70 +1.8 668,992 + cobC b0638 0.77 -1.4 669,031 + cobC b0638 0.84 +0.9 669,048 + cobC b0638 0.86 +2.1 669,057 - cobC b0638 0.88 +2.1 669,057 - cobC b0638 0.88 +0.4 669,095 - -1.4 669,153 - -1.2 669,158 - -1.1 669,158 - -1.3 669,179 + -1.9 669,187 - +1.2 669,852 + -3.5 670,785 + -0.8 670,837 - -0.5 670,880 - +1.2 670,915 - rlpB b0641 0.15 +0.1 670,922 - rlpB b0641 0.16 -1.4 671,394 - -0.8 671,404 - +0.3 671,404 - -2.1
Or see this region's nucleotide sequence