Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rna and citT are separated by 113 nucleotides citT and citG are separated by 50 nucleotides citG and citX overlap by 26 nucleotides
b0611: rna - ribonuclease I (NCBI), at 643,420 to 644,226
rna
b0612: citT - citrate:succinate antiporter (NCBI), at 644,340 to 645,803
citT
b0613: citG - triphosphoribosyl-dephospho-CoA transferase (NCBI), at 645,854 to 646,732
citG
b0614: citX - 2'-(5'-triphosphoribosyl)-3'-dephospho-CoA:apo- citrate lyase (NCBI), at 646,707 to 647,258
citX
Position (kb) 644
645
646 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 643.347 kb on - strand at 643.530 kb on + strand, within rna at 643.726 kb on - strand, within rna at 643.817 kb on + strand, within rna at 643.950 kb on - strand, within rna at 643.967 kb on + strand, within rna at 644.107 kb on + strand, within rna at 644.115 kb on - strand, within rna at 644.115 kb on - strand, within rna at 644.115 kb on - strand, within rna at 644.244 kb on + strand at 644.244 kb on + strand at 644.244 kb on + strand at 644.248 kb on - strand at 644.248 kb on - strand at 644.335 kb on - strand at 644.387 kb on - strand at 644.506 kb on + strand, within citT at 644.630 kb on + strand, within citT at 644.690 kb on + strand, within citT at 644.690 kb on + strand, within citT at 644.704 kb on - strand, within citT at 644.704 kb on - strand, within citT at 644.779 kb on + strand, within citT at 644.787 kb on - strand, within citT at 644.802 kb on - strand, within citT at 644.802 kb on - strand, within citT at 644.848 kb on - strand, within citT at 644.870 kb on + strand, within citT at 644.875 kb on - strand, within citT at 644.906 kb on + strand, within citT at 644.916 kb on - strand, within citT at 644.919 kb on - strand, within citT at 645.001 kb on + strand, within citT at 645.095 kb on - strand, within citT at 645.155 kb on - strand, within citT at 645.155 kb on - strand, within citT at 645.163 kb on - strand, within citT at 645.318 kb on - strand, within citT at 645.325 kb on + strand, within citT at 645.376 kb on - strand, within citT at 645.376 kb on - strand, within citT at 645.482 kb on + strand, within citT at 645.482 kb on + strand, within citT at 645.691 kb on + strand at 645.691 kb on + strand at 645.720 kb on + strand at 645.800 kb on + strand at 645.889 kb on + strand at 645.968 kb on + strand, within citG at 646.007 kb on + strand, within citG at 646.007 kb on + strand, within citG at 646.019 kb on + strand, within citG at 646.132 kb on + strand, within citG at 646.308 kb on - strand, within citG at 646.308 kb on - strand, within citG at 646.530 kb on - strand, within citG at 646.595 kb on + strand, within citG at 646.595 kb on + strand, within citG at 646.656 kb on + strand at 646.666 kb on + strand at 646.666 kb on + strand at 646.675 kb on - strand at 646.675 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 643,347 - -1.0 643,530 + rna b0611 0.14 +0.2 643,726 - rna b0611 0.38 -2.2 643,817 + rna b0611 0.49 -2.6 643,950 - rna b0611 0.66 -1.4 643,967 + rna b0611 0.68 -1.4 644,107 + rna b0611 0.85 -2.4 644,115 - rna b0611 0.86 +1.2 644,115 - rna b0611 0.86 +0.2 644,115 - rna b0611 0.86 -0.8 644,244 + +2.6 644,244 + -0.6 644,244 + +0.2 644,248 - +1.4 644,248 - -0.4 644,335 - +1.1 644,387 - +3.3 644,506 + citT b0612 0.11 +2.4 644,630 + citT b0612 0.20 -0.8 644,690 + citT b0612 0.24 +0.2 644,690 + citT b0612 0.24 +0.5 644,704 - citT b0612 0.25 -0.2 644,704 - citT b0612 0.25 -0.9 644,779 + citT b0612 0.30 +0.4 644,787 - citT b0612 0.31 -1.6 644,802 - citT b0612 0.32 -1.8 644,802 - citT b0612 0.32 +1.1 644,848 - citT b0612 0.35 +1.9 644,870 + citT b0612 0.36 +0.1 644,875 - citT b0612 0.37 -2.4 644,906 + citT b0612 0.39 +1.8 644,916 - citT b0612 0.39 +2.1 644,919 - citT b0612 0.40 +3.4 645,001 + citT b0612 0.45 +1.6 645,095 - citT b0612 0.52 -1.8 645,155 - citT b0612 0.56 -0.8 645,155 - citT b0612 0.56 +0.2 645,163 - citT b0612 0.56 +0.2 645,318 - citT b0612 0.67 +1.6 645,325 + citT b0612 0.67 +1.6 645,376 - citT b0612 0.71 -1.8 645,376 - citT b0612 0.71 -0.8 645,482 + citT b0612 0.78 +0.8 645,482 + citT b0612 0.78 +3.2 645,691 + -1.6 645,691 + -1.6 645,720 + +0.5 645,800 + +1.2 645,889 + -0.2 645,968 + citG b0613 0.13 +0.2 646,007 + citG b0613 0.17 +1.7 646,007 + citG b0613 0.17 -0.4 646,019 + citG b0613 0.19 -1.7 646,132 + citG b0613 0.32 -0.8 646,308 - citG b0613 0.52 +2.6 646,308 - citG b0613 0.52 -1.6 646,530 - citG b0613 0.77 -0.3 646,595 + citG b0613 0.84 -0.4 646,595 + citG b0613 0.84 +0.0 646,656 + +3.2 646,666 + +0.2 646,666 + -0.4 646,675 - +1.8 646,675 - -2.4
Or see this region's nucleotide sequence