Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cstA and ybdD are separated by 182 nucleotides ybdD and ybdH are separated by 9 nucleotides ybdH and ybdL are separated by 108 nucleotides
b0598: cstA - carbon starvation protein (NCBI), at 629,117 to 631,222
cstA
b4512: ybdD - hypothetical protein (NCBI), at 631,405 to 631,602
ybdD
b0599: ybdH - predicted oxidoreductase (NCBI), at 631,612 to 632,700
ybdH
b0600: ybdL - putative aminotransferase (NCBI), at 632,809 to 633,969
ybdL
Position (kb) 631
632
633 Strain fitness (log2 ratio) -2
-1
0
1
2 at 630.640 kb on + strand, within cstA at 630.703 kb on + strand, within cstA at 630.766 kb on + strand, within cstA at 630.816 kb on + strand, within cstA at 630.834 kb on - strand, within cstA at 630.834 kb on - strand, within cstA at 630.853 kb on - strand, within cstA at 630.865 kb on + strand, within cstA at 630.865 kb on + strand, within cstA at 630.894 kb on - strand, within cstA at 630.927 kb on - strand, within cstA at 630.927 kb on - strand, within cstA at 631.000 kb on + strand, within cstA at 631.000 kb on + strand, within cstA at 631.003 kb on + strand, within cstA at 631.068 kb on + strand at 631.068 kb on + strand at 631.126 kb on + strand at 631.180 kb on - strand at 631.194 kb on + strand at 631.194 kb on + strand at 631.236 kb on - strand at 631.250 kb on + strand at 631.262 kb on + strand at 631.262 kb on + strand at 631.264 kb on + strand at 631.278 kb on - strand at 631.300 kb on - strand at 631.300 kb on - strand at 631.305 kb on + strand at 631.398 kb on + strand at 631.398 kb on + strand at 631.401 kb on - strand at 631.413 kb on - strand at 631.413 kb on - strand at 631.780 kb on - strand, within ybdH at 631.782 kb on + strand, within ybdH at 631.782 kb on + strand, within ybdH at 631.825 kb on + strand, within ybdH at 631.828 kb on - strand, within ybdH at 631.828 kb on - strand, within ybdH at 631.833 kb on - strand, within ybdH at 631.833 kb on - strand, within ybdH at 631.926 kb on + strand, within ybdH at 631.926 kb on - strand, within ybdH at 632.035 kb on + strand, within ybdH at 632.048 kb on + strand, within ybdH at 632.147 kb on + strand, within ybdH at 632.160 kb on + strand, within ybdH at 632.399 kb on + strand, within ybdH at 632.466 kb on - strand, within ybdH at 632.581 kb on + strand, within ybdH at 632.877 kb on - strand at 632.877 kb on - strand at 632.948 kb on + strand, within ybdL at 633.223 kb on + strand, within ybdL at 633.228 kb on + strand, within ybdL at 633.228 kb on + strand, within ybdL at 633.228 kb on - strand, within ybdL at 633.320 kb on - strand, within ybdL at 633.322 kb on - strand, within ybdL at 633.327 kb on - strand, within ybdL at 633.351 kb on - strand, within ybdL at 633.418 kb on + strand, within ybdL at 633.418 kb on - strand, within ybdL at 633.418 kb on - strand, within ybdL at 633.442 kb on - strand, within ybdL at 633.442 kb on - strand, within ybdL at 633.541 kb on - strand, within ybdL at 633.551 kb on - strand, within ybdL at 633.570 kb on - strand, within ybdL at 633.572 kb on + strand, within ybdL at 633.587 kb on + strand, within ybdL at 633.597 kb on - strand, within ybdL
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 630,640 + cstA b0598 0.72 +1.4 630,703 + cstA b0598 0.75 +0.7 630,766 + cstA b0598 0.78 +1.2 630,816 + cstA b0598 0.81 -0.4 630,834 - cstA b0598 0.82 +2.5 630,834 - cstA b0598 0.82 -0.9 630,853 - cstA b0598 0.82 -0.3 630,865 + cstA b0598 0.83 -1.6 630,865 + cstA b0598 0.83 -0.4 630,894 - cstA b0598 0.84 +0.5 630,927 - cstA b0598 0.86 -0.8 630,927 - cstA b0598 0.86 +0.8 631,000 + cstA b0598 0.89 -1.9 631,000 + cstA b0598 0.89 +0.9 631,003 + cstA b0598 0.90 -1.4 631,068 + -0.5 631,068 + -0.2 631,126 + -0.5 631,180 - -0.8 631,194 + -1.4 631,194 + +1.0 631,236 - -1.6 631,250 + -1.4 631,262 + +0.6 631,262 + -0.4 631,264 + +1.0 631,278 - +1.2 631,300 - +1.3 631,300 - -0.5 631,305 + -0.4 631,398 + +2.1 631,398 + +2.2 631,401 - +0.2 631,413 - +1.6 631,413 - +0.3 631,780 - ybdH b0599 0.15 -1.6 631,782 + ybdH b0599 0.16 -1.4 631,782 + ybdH b0599 0.16 +1.4 631,825 + ybdH b0599 0.20 -1.5 631,828 - ybdH b0599 0.20 +0.7 631,828 - ybdH b0599 0.20 +0.6 631,833 - ybdH b0599 0.20 -2.2 631,833 - ybdH b0599 0.20 -1.6 631,926 + ybdH b0599 0.29 +1.0 631,926 - ybdH b0599 0.29 -1.1 632,035 + ybdH b0599 0.39 -0.4 632,048 + ybdH b0599 0.40 +1.1 632,147 + ybdH b0599 0.49 -0.6 632,160 + ybdH b0599 0.50 +0.7 632,399 + ybdH b0599 0.72 +0.2 632,466 - ybdH b0599 0.78 -1.1 632,581 + ybdH b0599 0.89 +0.2 632,877 - -1.4 632,877 - -0.8 632,948 + ybdL b0600 0.12 +2.1 633,223 + ybdL b0600 0.36 -0.2 633,228 + ybdL b0600 0.36 -1.4 633,228 + ybdL b0600 0.36 +1.2 633,228 - ybdL b0600 0.36 -1.7 633,320 - ybdL b0600 0.44 -0.1 633,322 - ybdL b0600 0.44 +0.1 633,327 - ybdL b0600 0.45 +1.6 633,351 - ybdL b0600 0.47 +0.2 633,418 + ybdL b0600 0.52 -0.8 633,418 - ybdL b0600 0.52 -1.9 633,418 - ybdL b0600 0.52 -0.7 633,442 - ybdL b0600 0.55 +1.7 633,442 - ybdL b0600 0.55 -2.1 633,541 - ybdL b0600 0.63 -0.6 633,551 - ybdL b0600 0.64 +1.2 633,570 - ybdL b0600 0.66 -0.2 633,572 + ybdL b0600 0.66 -0.5 633,587 + ybdL b0600 0.67 +1.7 633,597 - ybdL b0600 0.68 +0.8
Or see this region's nucleotide sequence