Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fepD and ybdA are separated by 110 nucleotides ybdA and fepB are separated by 3 nucleotides
b0590: fepD - iron-enterobactin transporter subunit (NCBI), at 620,408 to 621,412
fepD
b0591: ybdA - predicted transporter (NCBI), at 621,523 to 622,773
ybdA
b0592: fepB - iron-enterobactin transporter subunit (NCBI), at 622,777 to 623,733
fepB
Position (kb)
621
622
623 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 620.634 kb on - strand, within fepD at 620.653 kb on - strand, within fepD at 620.653 kb on - strand, within fepD at 620.664 kb on - strand, within fepD at 620.672 kb on - strand, within fepD at 620.728 kb on - strand, within fepD at 620.749 kb on + strand, within fepD at 620.749 kb on + strand, within fepD at 620.749 kb on + strand, within fepD at 620.780 kb on - strand, within fepD at 620.808 kb on + strand, within fepD at 620.808 kb on + strand, within fepD at 620.892 kb on + strand, within fepD at 620.900 kb on - strand, within fepD at 620.964 kb on - strand, within fepD at 620.964 kb on - strand, within fepD at 620.970 kb on + strand, within fepD at 620.970 kb on + strand, within fepD at 621.018 kb on - strand, within fepD at 621.228 kb on - strand, within fepD at 621.295 kb on - strand, within fepD at 621.295 kb on - strand, within fepD at 621.331 kb on + strand at 621.474 kb on - strand at 621.474 kb on - strand at 621.487 kb on - strand at 621.608 kb on + strand at 621.654 kb on - strand, within ybdA at 621.727 kb on - strand, within ybdA at 621.819 kb on + strand, within ybdA at 621.846 kb on + strand, within ybdA at 621.846 kb on + strand, within ybdA at 621.966 kb on + strand, within ybdA at 622.236 kb on + strand, within ybdA at 622.246 kb on + strand, within ybdA at 622.255 kb on - strand, within ybdA at 622.255 kb on - strand, within ybdA at 622.365 kb on + strand, within ybdA at 622.365 kb on + strand, within ybdA at 622.409 kb on - strand, within ybdA at 622.434 kb on + strand, within ybdA at 622.441 kb on - strand, within ybdA at 622.441 kb on - strand, within ybdA at 622.472 kb on + strand, within ybdA at 622.472 kb on + strand, within ybdA at 622.491 kb on + strand, within ybdA at 622.596 kb on + strand, within ybdA at 622.596 kb on + strand, within ybdA at 622.611 kb on - strand, within ybdA at 622.611 kb on - strand, within ybdA at 622.615 kb on + strand, within ybdA at 622.615 kb on + strand, within ybdA at 622.801 kb on - strand at 622.819 kb on + strand at 622.819 kb on + strand at 622.847 kb on - strand at 622.875 kb on + strand, within fepB at 622.875 kb on + strand, within fepB at 622.899 kb on + strand, within fepB at 622.917 kb on + strand, within fepB at 622.923 kb on + strand, within fepB at 622.958 kb on + strand, within fepB at 623.019 kb on + strand, within fepB at 623.019 kb on + strand, within fepB at 623.028 kb on - strand, within fepB at 623.028 kb on - strand, within fepB at 623.032 kb on + strand, within fepB at 623.119 kb on + strand, within fepB at 623.140 kb on - strand, within fepB at 623.148 kb on - strand, within fepB at 623.148 kb on - strand, within fepB at 623.199 kb on - strand, within fepB at 623.253 kb on - strand, within fepB at 623.341 kb on - strand, within fepB at 623.405 kb on - strand, within fepB at 623.405 kb on - strand, within fepB at 623.426 kb on + strand, within fepB at 623.426 kb on + strand, within fepB at 623.458 kb on - strand, within fepB at 623.458 kb on - strand, within fepB at 623.467 kb on - strand, within fepB at 623.467 kb on - strand, within fepB at 623.470 kb on - strand, within fepB at 623.470 kb on - strand, within fepB at 623.563 kb on + strand, within fepB at 623.576 kb on - strand, within fepB at 623.576 kb on - strand, within fepB at 623.591 kb on - strand, within fepB at 623.591 kb on - strand, within fepB at 623.610 kb on + strand, within fepB at 623.672 kb on + strand at 623.708 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 620,634 - fepD b0590 0.22 +0.1 620,653 - fepD b0590 0.24 +0.6 620,653 - fepD b0590 0.24 +0.2 620,664 - fepD b0590 0.25 +0.6 620,672 - fepD b0590 0.26 -2.7 620,728 - fepD b0590 0.32 -1.1 620,749 + fepD b0590 0.34 -0.0 620,749 + fepD b0590 0.34 -0.5 620,749 + fepD b0590 0.34 -0.4 620,780 - fepD b0590 0.37 -0.4 620,808 + fepD b0590 0.40 +1.2 620,808 + fepD b0590 0.40 -0.2 620,892 + fepD b0590 0.48 +1.2 620,900 - fepD b0590 0.49 -1.4 620,964 - fepD b0590 0.55 -0.8 620,964 - fepD b0590 0.55 +4.7 620,970 + fepD b0590 0.56 -0.9 620,970 + fepD b0590 0.56 +1.3 621,018 - fepD b0590 0.61 +1.2 621,228 - fepD b0590 0.82 +0.6 621,295 - fepD b0590 0.88 +2.3 621,295 - fepD b0590 0.88 -2.1 621,331 + +1.2 621,474 - -1.2 621,474 - -0.9 621,487 - -0.4 621,608 + +0.3 621,654 - ybdA b0591 0.10 -0.1 621,727 - ybdA b0591 0.16 +1.2 621,819 + ybdA b0591 0.24 -0.1 621,846 + ybdA b0591 0.26 -0.4 621,846 + ybdA b0591 0.26 -1.4 621,966 + ybdA b0591 0.35 -0.4 622,236 + ybdA b0591 0.57 -0.8 622,246 + ybdA b0591 0.58 -0.8 622,255 - ybdA b0591 0.59 -0.9 622,255 - ybdA b0591 0.59 -0.0 622,365 + ybdA b0591 0.67 +0.5 622,365 + ybdA b0591 0.67 -0.4 622,409 - ybdA b0591 0.71 +0.8 622,434 + ybdA b0591 0.73 -1.8 622,441 - ybdA b0591 0.73 -1.8 622,441 - ybdA b0591 0.73 +1.3 622,472 + ybdA b0591 0.76 -2.2 622,472 + ybdA b0591 0.76 +0.3 622,491 + ybdA b0591 0.77 -0.4 622,596 + ybdA b0591 0.86 +0.2 622,596 + ybdA b0591 0.86 -0.3 622,611 - ybdA b0591 0.87 -1.1 622,611 - ybdA b0591 0.87 -0.8 622,615 + ybdA b0591 0.87 -1.1 622,615 + ybdA b0591 0.87 -1.9 622,801 - +3.0 622,819 + +1.9 622,819 + +1.5 622,847 - +1.6 622,875 + fepB b0592 0.10 +2.4 622,875 + fepB b0592 0.10 +1.2 622,899 + fepB b0592 0.13 +0.2 622,917 + fepB b0592 0.15 +0.9 622,923 + fepB b0592 0.15 +1.6 622,958 + fepB b0592 0.19 -0.2 623,019 + fepB b0592 0.25 -1.1 623,019 + fepB b0592 0.25 -0.4 623,028 - fepB b0592 0.26 +2.0 623,028 - fepB b0592 0.26 +0.0 623,032 + fepB b0592 0.27 +2.3 623,119 + fepB b0592 0.36 -0.4 623,140 - fepB b0592 0.38 -1.5 623,148 - fepB b0592 0.39 +0.6 623,148 - fepB b0592 0.39 +1.2 623,199 - fepB b0592 0.44 -1.0 623,253 - fepB b0592 0.50 +1.4 623,341 - fepB b0592 0.59 -0.4 623,405 - fepB b0592 0.66 -0.1 623,405 - fepB b0592 0.66 +0.2 623,426 + fepB b0592 0.68 -0.8 623,426 + fepB b0592 0.68 -0.0 623,458 - fepB b0592 0.71 -1.8 623,458 - fepB b0592 0.71 +0.1 623,467 - fepB b0592 0.72 +0.4 623,467 - fepB b0592 0.72 +0.2 623,470 - fepB b0592 0.72 -2.6 623,470 - fepB b0592 0.72 -0.7 623,563 + fepB b0592 0.82 +0.7 623,576 - fepB b0592 0.83 +1.8 623,576 - fepB b0592 0.83 +0.2 623,591 - fepB b0592 0.85 -0.9 623,591 - fepB b0592 0.85 -1.0 623,610 + fepB b0592 0.87 -0.9 623,672 + -0.2 623,708 + -1.6
Or see this region's nucleotide sequence