Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt insL-2 and entD are separated by 281 nucleotides entD and fepA are separated by 174 nucleotides
b0582: insL-2 - IS186/IS421 transposase (NCBI), at 607,288 to 608,400
insL-2
b0583: entD - phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex (NCBI), at 608,682 to 609,302
entD
b0584: fepA - iron-enterobactin outer membrane transporter (NCBI), at 609,477 to 611,717
fepA
Position (kb) 608
609
610 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 608.621 kb on + strand at 608.621 kb on + strand at 608.621 kb on + strand at 608.629 kb on - strand at 608.669 kb on - strand at 608.750 kb on - strand, within entD at 608.761 kb on + strand, within entD at 608.766 kb on + strand, within entD at 608.834 kb on + strand, within entD at 609.183 kb on + strand, within entD at 609.246 kb on - strand at 609.246 kb on - strand at 609.253 kb on + strand at 609.253 kb on + strand at 609.266 kb on + strand at 609.266 kb on + strand at 609.277 kb on - strand at 609.277 kb on - strand at 609.284 kb on - strand at 609.303 kb on - strand at 609.304 kb on - strand at 609.338 kb on - strand at 609.338 kb on - strand at 609.498 kb on + strand at 609.536 kb on - strand at 609.549 kb on - strand at 609.580 kb on + strand at 609.597 kb on - strand at 609.597 kb on - strand at 609.724 kb on - strand, within fepA at 609.966 kb on - strand, within fepA at 610.025 kb on + strand, within fepA at 610.193 kb on - strand, within fepA at 610.193 kb on - strand, within fepA at 610.202 kb on - strand, within fepA
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 608,621 + +1.9 608,621 + -0.4 608,621 + +3.2 608,629 - -0.9 608,669 - +1.5 608,750 - entD b0583 0.11 +0.3 608,761 + entD b0583 0.13 +1.0 608,766 + entD b0583 0.14 -0.1 608,834 + entD b0583 0.24 -0.4 609,183 + entD b0583 0.81 -1.8 609,246 - -1.4 609,246 - -0.4 609,253 + +0.4 609,253 + +2.7 609,266 + +0.4 609,266 + +0.2 609,277 - +0.9 609,277 - -0.8 609,284 - +0.1 609,303 - -0.8 609,304 - +1.2 609,338 - -2.4 609,338 - -0.1 609,498 + +1.8 609,536 - -1.5 609,549 - -2.5 609,580 + -0.9 609,597 - -0.9 609,597 - +0.2 609,724 - fepA b0584 0.11 +1.0 609,966 - fepA b0584 0.22 +0.7 610,025 + fepA b0584 0.24 +0.9 610,193 - fepA b0584 0.32 -1.6 610,193 - fepA b0584 0.32 -0.4 610,202 - fepA b0584 0.32 -2.9
Or see this region's nucleotide sequence