Strain Fitness in Escherichia coli BW25113 around b0546

Experiment: Cisplatin 0.1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntybcL and ybcM are separated by 9 nucleotidesybcM and ylcH are separated by 116 nucleotidesylcH and ybcN overlap by 4 nucleotidesybcN and ninE overlap by 1 nucleotidesninE and ybcO overlap by 8 nucleotides b0545: ybcL - DLP12 prophage; predicted kinase inhibitor (NCBI), at 570,116 to 570,667 ybcL b0546: ybcM - DLP12 prophage; predicted DNA-binding transcriptional regulator (NCBI), at 570,677 to 571,474 ybcM b4588: ylcH - hypothetical protein, DLP12 prophage (RefSeq), at 571,591 to 571,692 ylcH b0547: ybcN - DLP12 prophage; predicted protein (NCBI), at 571,689 to 572,144 ybcN b0548: ninE - DLP12 prophage; conserved protein (NCBI), at 572,144 to 572,314 ninE b0549: ybcO - DLP12 prophage; predicted protein (NCBI), at 572,307 to 572,597 ybcO Position (kb) 570 571 572Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 569.954 kb on - strandat 569.991 kb on - strandat 569.991 kb on - strandat 570.177 kb on - strand, within ybcLat 570.182 kb on - strand, within ybcLat 570.217 kb on + strand, within ybcLat 570.217 kb on - strand, within ybcLat 570.225 kb on - strand, within ybcLat 570.235 kb on - strand, within ybcLat 570.236 kb on - strand, within ybcLat 570.259 kb on + strand, within ybcLat 570.265 kb on - strand, within ybcLat 570.267 kb on - strand, within ybcLat 570.273 kb on - strand, within ybcLat 570.276 kb on + strand, within ybcLat 570.283 kb on - strand, within ybcLat 570.289 kb on - strand, within ybcLat 570.289 kb on - strandat 570.293 kb on + strand, within ybcLat 570.295 kb on + strand, within ybcLat 570.296 kb on + strand, within ybcLat 570.302 kb on - strand, within ybcLat 570.302 kb on - strand, within ybcLat 570.305 kb on + strand, within ybcLat 570.305 kb on + strand, within ybcLat 570.312 kb on + strand, within ybcLat 570.323 kb on + strand, within ybcLat 570.323 kb on + strand, within ybcLat 570.332 kb on - strand, within ybcLat 570.342 kb on + strand, within ybcLat 570.343 kb on + strand, within ybcLat 570.343 kb on + strand, within ybcLat 570.344 kb on + strand, within ybcLat 570.350 kb on - strand, within ybcLat 570.351 kb on - strand, within ybcLat 570.354 kb on - strand, within ybcLat 570.356 kb on + strand, within ybcLat 570.364 kb on + strand, within ybcLat 570.364 kb on + strand, within ybcLat 570.372 kb on - strand, within ybcLat 570.374 kb on + strand, within ybcLat 570.374 kb on + strand, within ybcLat 570.382 kb on - strand, within ybcLat 570.401 kb on + strand, within ybcLat 570.401 kb on + strand, within ybcLat 570.409 kb on + strand, within ybcLat 570.418 kb on + strand, within ybcLat 570.440 kb on + strand, within ybcLat 570.445 kb on + strand, within ybcLat 570.450 kb on + strand, within ybcLat 570.455 kb on + strand, within ybcLat 570.455 kb on + strand, within ybcLat 570.455 kb on + strand, within ybcLat 570.458 kb on - strand, within ybcLat 570.480 kb on + strand, within ybcLat 570.482 kb on + strand, within ybcLat 570.488 kb on - strand, within ybcLat 570.496 kb on + strand, within ybcLat 570.496 kb on + strand, within ybcLat 570.501 kb on + strand, within ybcLat 570.501 kb on + strand, within ybcLat 570.505 kb on + strand, within ybcLat 570.514 kb on + strand, within ybcLat 570.535 kb on + strand, within ybcLat 570.535 kb on + strand, within ybcLat 570.535 kb on + strand, within ybcLat 570.535 kb on + strand, within ybcLat 570.535 kb on + strand, within ybcLat 570.535 kb on + strand, within ybcLat 570.566 kb on + strand, within ybcLat 570.576 kb on + strand, within ybcLat 570.581 kb on + strand, within ybcLat 570.625 kb on + strandat 570.644 kb on + strandat 570.650 kb on + strandat 570.650 kb on + strandat 570.661 kb on + strandat 570.665 kb on - strandat 570.668 kb on + strandat 570.668 kb on + strandat 570.672 kb on + strandat 570.675 kb on - strandat 570.675 kb on - strandat 570.675 kb on - strandat 570.682 kb on + strandat 570.682 kb on + strandat 570.688 kb on + strandat 570.688 kb on + strandat 570.697 kb on - strandat 570.772 kb on + strand, within ybcMat 570.772 kb on + strand, within ybcMat 570.804 kb on + strand, within ybcMat 570.804 kb on + strand, within ybcMat 570.804 kb on + strand, within ybcMat 570.804 kb on + strand, within ybcMat 570.804 kb on + strand, within ybcMat 570.817 kb on + strand, within ybcMat 570.992 kb on + strand, within ybcMat 570.992 kb on + strand, within ybcMat 571.055 kb on + strand, within ybcMat 571.084 kb on - strand, within ybcMat 571.094 kb on - strand, within ybcMat 571.105 kb on + strand, within ybcMat 571.115 kb on + strand, within ybcMat 571.124 kb on - strand, within ybcMat 571.130 kb on - strand, within ybcMat 571.170 kb on - strand, within ybcMat 571.186 kb on + strand, within ybcMat 571.207 kb on + strand, within ybcMat 571.207 kb on + strand, within ybcMat 571.215 kb on - strand, within ybcMat 571.226 kb on + strand, within ybcMat 571.283 kb on + strand, within ybcMat 571.301 kb on + strand, within ybcMat 571.301 kb on + strand, within ybcMat 571.306 kb on + strand, within ybcMat 571.340 kb on + strand, within ybcMat 571.340 kb on + strand, within ybcMat 571.340 kb on + strand, within ybcMat 571.341 kb on - strand, within ybcMat 571.345 kb on - strand, within ybcMat 571.418 kb on + strandat 571.427 kb on + strandat 571.477 kb on - strandat 571.486 kb on - strandat 571.546 kb on - strandat 571.560 kb on - strandat 571.575 kb on - strandat 571.575 kb on - strandat 571.585 kb on - strandat 571.596 kb on + strandat 571.596 kb on - strandat 571.596 kb on - strandat 571.602 kb on - strand, within ylcHat 571.618 kb on + strand, within ylcHat 571.623 kb on - strand, within ylcHat 571.629 kb on + strand, within ylcHat 571.638 kb on - strand, within ylcHat 571.671 kb on + strand, within ylcHat 571.681 kb on + strand, within ylcHat 571.681 kb on + strand, within ylcHat 571.682 kb on - strandat 571.682 kb on - strandat 571.682 kb on - strandat 571.682 kb on - strandat 571.682 kb on - strandat 571.682 kb on - strandat 571.682 kb on - strandat 571.687 kb on + strandat 571.694 kb on - strandat 571.695 kb on - strandat 571.752 kb on + strand, within ybcNat 571.752 kb on + strand, within ybcNat 571.909 kb on + strand, within ybcNat 571.931 kb on + strand, within ybcNat 571.937 kb on - strand, within ybcNat 571.939 kb on - strand, within ybcNat 571.939 kb on - strand, within ybcNat 572.055 kb on + strand, within ybcNat 572.068 kb on - strand, within ybcNat 572.179 kb on + strand, within ninEat 572.179 kb on + strand, within ninEat 572.179 kb on + strand, within ninEat 572.195 kb on - strand, within ninEat 572.214 kb on - strand, within ninEat 572.343 kb on - strand, within ybcO

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Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml
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569,954 - -2.4
569,991 - +2.2
569,991 - +2.2
570,177 - ybcL b0545 0.11 +1.0
570,182 - ybcL b0545 0.12 +0.7
570,217 + ybcL b0545 0.18 -2.1
570,217 - ybcL b0545 0.18 +1.0
570,225 - ybcL b0545 0.20 -0.7
570,235 - ybcL b0545 0.22 +1.2
570,236 - ybcL b0545 0.22 -1.6
570,259 + ybcL b0545 0.26 -0.9
570,265 - ybcL b0545 0.27 -0.8
570,267 - ybcL b0545 0.27 -2.2
570,273 - ybcL b0545 0.28 -1.3
570,276 + ybcL b0545 0.29 -3.2
570,283 - ybcL b0545 0.30 -1.1
570,289 - ybcL b0545 0.31 -1.6
570,289 - +1.2
570,293 + ybcL b0545 0.32 -1.1
570,295 + ybcL b0545 0.32 +0.2
570,296 + ybcL b0545 0.33 +1.7
570,302 - ybcL b0545 0.34 +2.2
570,302 - ybcL b0545 0.34 -1.6
570,305 + ybcL b0545 0.34 +1.2
570,305 + ybcL b0545 0.34 +1.5
570,312 + ybcL b0545 0.36 +0.8
570,323 + ybcL b0545 0.38 +0.2
570,323 + ybcL b0545 0.38 -0.4
570,332 - ybcL b0545 0.39 -0.4
570,342 + ybcL b0545 0.41 -0.8
570,343 + ybcL b0545 0.41 +0.8
570,343 + ybcL b0545 0.41 +1.1
570,344 + ybcL b0545 0.41 +1.8
570,350 - ybcL b0545 0.42 -0.8
570,351 - ybcL b0545 0.43 -0.4
570,354 - ybcL b0545 0.43 -0.4
570,356 + ybcL b0545 0.43 -0.4
570,364 + ybcL b0545 0.45 -0.8
570,364 + ybcL b0545 0.45 -0.8
570,372 - ybcL b0545 0.46 -0.9
570,374 + ybcL b0545 0.47 -1.9
570,374 + ybcL b0545 0.47 -1.9
570,382 - ybcL b0545 0.48 -1.1
570,401 + ybcL b0545 0.52 -0.4
570,401 + ybcL b0545 0.52 +0.2
570,409 + ybcL b0545 0.53 -0.4
570,418 + ybcL b0545 0.55 -0.1
570,440 + ybcL b0545 0.59 +2.5
570,445 + ybcL b0545 0.60 -0.4
570,450 + ybcL b0545 0.61 -0.5
570,455 + ybcL b0545 0.61 +3.7
570,455 + ybcL b0545 0.61 -1.6
570,455 + ybcL b0545 0.61 -1.1
570,458 - ybcL b0545 0.62 -1.3
570,480 + ybcL b0545 0.66 -0.7
570,482 + ybcL b0545 0.66 +0.2
570,488 - ybcL b0545 0.67 -2.5
570,496 + ybcL b0545 0.69 -2.7
570,496 + ybcL b0545 0.69 +1.0
570,501 + ybcL b0545 0.70 -0.1
570,501 + ybcL b0545 0.70 -1.1
570,505 + ybcL b0545 0.70 -0.6
570,514 + ybcL b0545 0.72 -0.5
570,535 + ybcL b0545 0.76 -1.9
570,535 + ybcL b0545 0.76 +0.9
570,535 + ybcL b0545 0.76 +0.2
570,535 + ybcL b0545 0.76 +1.4
570,535 + ybcL b0545 0.76 +0.2
570,535 + ybcL b0545 0.76 -1.1
570,566 + ybcL b0545 0.82 +0.2
570,576 + ybcL b0545 0.83 -1.6
570,581 + ybcL b0545 0.84 -0.7
570,625 + +1.3
570,644 + +0.6
570,650 + -0.8
570,650 + -0.3
570,661 + -0.4
570,665 - -2.5
570,668 + -2.1
570,668 + +0.1
570,672 + -2.2
570,675 - -1.6
570,675 - -2.6
570,675 - -0.8
570,682 + -0.8
570,682 + -2.1
570,688 + +0.4
570,688 + +2.5
570,697 - +0.3
570,772 + ybcM b0546 0.12 -1.4
570,772 + ybcM b0546 0.12 +0.5
570,804 + ybcM b0546 0.16 -0.8
570,804 + ybcM b0546 0.16 -1.4
570,804 + ybcM b0546 0.16 -1.4
570,804 + ybcM b0546 0.16 -0.8
570,804 + ybcM b0546 0.16 -0.4
570,817 + ybcM b0546 0.18 -0.8
570,992 + ybcM b0546 0.39 -0.6
570,992 + ybcM b0546 0.39 +3.6
571,055 + ybcM b0546 0.47 -0.7
571,084 - ybcM b0546 0.51 -2.4
571,094 - ybcM b0546 0.52 -2.5
571,105 + ybcM b0546 0.54 -0.6
571,115 + ybcM b0546 0.55 -1.8
571,124 - ybcM b0546 0.56 +2.0
571,130 - ybcM b0546 0.57 -0.9
571,170 - ybcM b0546 0.62 +0.8
571,186 + ybcM b0546 0.64 -0.2
571,207 + ybcM b0546 0.66 +0.2
571,207 + ybcM b0546 0.66 -1.1
571,215 - ybcM b0546 0.67 -1.5
571,226 + ybcM b0546 0.69 -0.2
571,283 + ybcM b0546 0.76 -1.4
571,301 + ybcM b0546 0.78 +0.5
571,301 + ybcM b0546 0.78 +1.8
571,306 + ybcM b0546 0.79 +1.0
571,340 + ybcM b0546 0.83 -1.9
571,340 + ybcM b0546 0.83 -1.8
571,340 + ybcM b0546 0.83 -0.0
571,341 - ybcM b0546 0.83 -0.4
571,345 - ybcM b0546 0.84 +0.3
571,418 + -0.9
571,427 + -1.8
571,477 - -0.8
571,486 - +0.2
571,546 - +0.1
571,560 - -1.7
571,575 - +0.2
571,575 - +2.2
571,585 - -1.1
571,596 + +0.1
571,596 - -0.6
571,596 - +1.6
571,602 - ylcH b4588 0.11 -1.4
571,618 + ylcH b4588 0.26 +0.6
571,623 - ylcH b4588 0.31 +0.7
571,629 + ylcH b4588 0.37 -0.5
571,638 - ylcH b4588 0.46 -2.1
571,671 + ylcH b4588 0.78 +0.7
571,681 + ylcH b4588 0.88 +0.5
571,681 + ylcH b4588 0.88 -1.1
571,682 - +0.0
571,682 - +0.0
571,682 - +1.2
571,682 - +1.2
571,682 - -1.9
571,682 - -0.3
571,682 - +0.2
571,687 + -0.2
571,694 - +0.2
571,695 - -1.1
571,752 + ybcN b0547 0.14 +2.4
571,752 + ybcN b0547 0.14 +0.2
571,909 + ybcN b0547 0.48 +0.9
571,931 + ybcN b0547 0.53 -1.8
571,937 - ybcN b0547 0.54 -0.9
571,939 - ybcN b0547 0.55 -1.4
571,939 - ybcN b0547 0.55 -0.4
572,055 + ybcN b0547 0.80 -1.2
572,068 - ybcN b0547 0.83 +2.4
572,179 + ninE b0548 0.20 -1.6
572,179 + ninE b0548 0.20 -1.4
572,179 + ninE b0548 0.20 -0.8
572,195 - ninE b0548 0.30 +1.4
572,214 - ninE b0548 0.41 +1.3
572,343 - ybcO b0549 0.12 +0.5

Or see this region's nucleotide sequence