Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ylbE and ylbF are separated by 10 nucleotides ylbF and ybcF overlap by 4 nucleotides
b4572: ylbE - no description, at 547,581 to 548,839
ylbE
b0520: ylbF - hypothetical protein (NCBI), at 548,850 to 549,665
ylbF
b0521: ybcF - predicted carbamate kinase (NCBI), at 549,662 to 550,555
ybcF
Position (kb) 548
549
550 Strain fitness (log2 ratio) -2
-1
0
1
2 at 547.895 kb on - strand, within ylbE at 548.009 kb on + strand, within ylbE at 548.151 kb on - strand, within ylbE at 548.165 kb on + strand, within ylbE at 548.165 kb on + strand, within ylbE at 548.208 kb on - strand, within ylbE at 548.208 kb on - strand, within ylbE at 548.506 kb on + strand, within ylbE at 548.533 kb on + strand, within ylbE at 548.533 kb on + strand, within ylbE at 548.700 kb on + strand, within ylbE at 548.708 kb on - strand, within ylbE at 548.713 kb on - strand, within ylbE at 548.718 kb on - strand at 548.755 kb on + strand at 548.833 kb on + strand at 549.010 kb on + strand, within ylbF at 549.010 kb on + strand, within ylbF at 549.046 kb on - strand, within ylbF at 549.046 kb on - strand, within ylbF at 549.219 kb on + strand, within ylbF at 549.354 kb on - strand, within ylbF at 549.354 kb on - strand, within ylbF at 549.392 kb on + strand, within ylbF at 549.450 kb on + strand, within ylbF at 549.450 kb on + strand, within ylbF at 549.458 kb on + strand, within ylbF at 549.488 kb on + strand, within ylbF at 549.491 kb on - strand, within ylbF at 549.534 kb on + strand, within ylbF at 549.625 kb on + strand at 549.695 kb on + strand at 549.755 kb on + strand, within ybcF at 549.755 kb on + strand, within ybcF at 549.764 kb on + strand, within ybcF at 549.811 kb on - strand, within ybcF at 549.933 kb on + strand, within ybcF at 549.933 kb on + strand, within ybcF at 549.954 kb on - strand, within ybcF at 550.141 kb on - strand, within ybcF at 550.363 kb on + strand, within ybcF at 550.471 kb on - strand at 550.539 kb on + strand at 550.550 kb on - strand at 550.563 kb on + strand at 550.563 kb on - strand at 550.607 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 547,895 - ylbE b4572 0.25 -1.6 548,009 + ylbE b4572 0.34 -0.8 548,151 - ylbE b4572 0.45 -1.3 548,165 + ylbE b4572 0.46 +1.0 548,165 + ylbE b4572 0.46 -1.3 548,208 - ylbE b4572 0.50 -1.9 548,208 - ylbE b4572 0.50 +0.1 548,506 + ylbE b4572 0.73 -1.6 548,533 + ylbE b4572 0.76 +0.5 548,533 + ylbE b4572 0.76 -1.1 548,700 + ylbE b4572 0.89 +2.7 548,708 - ylbE b4572 0.90 -1.3 548,713 - ylbE b4572 0.90 -0.6 548,718 - +0.3 548,755 + +0.5 548,833 + +0.1 549,010 + ylbF b0520 0.20 -1.1 549,010 + ylbF b0520 0.20 -0.4 549,046 - ylbF b0520 0.24 -2.1 549,046 - ylbF b0520 0.24 -0.8 549,219 + ylbF b0520 0.45 -1.8 549,354 - ylbF b0520 0.62 -1.1 549,354 - ylbF b0520 0.62 +0.9 549,392 + ylbF b0520 0.66 -0.4 549,450 + ylbF b0520 0.74 -1.1 549,450 + ylbF b0520 0.74 -0.8 549,458 + ylbF b0520 0.75 -0.4 549,488 + ylbF b0520 0.78 -0.3 549,491 - ylbF b0520 0.79 -0.7 549,534 + ylbF b0520 0.84 +1.2 549,625 + +1.2 549,695 + +1.3 549,755 + ybcF b0521 0.10 -1.2 549,755 + ybcF b0521 0.10 -0.7 549,764 + ybcF b0521 0.11 +2.7 549,811 - ybcF b0521 0.17 -1.1 549,933 + ybcF b0521 0.30 +2.0 549,933 + ybcF b0521 0.30 +1.2 549,954 - ybcF b0521 0.33 +1.2 550,141 - ybcF b0521 0.54 +0.3 550,363 + ybcF b0521 0.78 -0.8 550,471 - -2.4 550,539 + +1.3 550,550 - -1.4 550,563 + +1.1 550,563 - +2.6 550,607 + +0.9
Or see this region's nucleotide sequence